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Preparation method of black tea fungus pellicle

A technology of kombucha fungus and biofilm, applied in the direction of microorganism-based methods, biochemical equipment and methods, tea extraction, etc., can solve the problems of poor taste, inefficient production, low yield, etc., to promote the formation of biofilm, weaken Inhibitory effect, effect of increasing growth

Inactive Publication Date: 2017-09-15
INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a method for preparing kombucha fungus film aiming at the problems of low output, poor taste and inefficient production of the existing kombucha fungus film

Method used

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  • Preparation method of black tea fungus pellicle
  • Preparation method of black tea fungus pellicle
  • Preparation method of black tea fungus pellicle

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preparation example Construction

[0013] A preparation method of kombucha bacterial film, comprising the following steps:

[0014] Step 1. Take black tea and extract it with water at 60-90°C twice for 10-30 minutes. The mass ratio of tea leaves to water is 1:(100-200). After primary filtration, mix the two extracts. Using 60-90°C water for two consecutive extractions for 10-30 minutes and then mixing the two extracts can not only obtain black tea extracts with a higher extraction rate, but also effectively reduce the heat-sensitive components in black tea from being destroyed at high temperatures, ensuring Its original flavor.

[0015] Step 2: Add black tea tannase with a mass ratio of 0.05-0.1% to the extract obtained in step 1, then enzymolyze it at 35-45°C for 60-90min, then heat at 90-100°C for 5- 10min to inactivate the enzyme, then cool to room temperature. The polyphenolic compounds in tea not only have strong antioxidant activity, but also have good antibacterial activity, especially ester-type catec...

Embodiment 1

[0025] Take black tea leaves and crush them, extract with pure water at 90°C for 25 minutes at a tea-to-water ratio of 1:150; after coarse filtration, extract the tea dregs at the same tea-to-water ratio for 15 minutes, then filter, and mix the two extracts. After the tea extract is cooled to 45°C, add 0.08% tea leaf weight tannase to the extract, enzymatically hydrolyze for 90 minutes, heat to inactivate the enzyme, and cool to room temperature. Then add 10% sucrose, boil for 10min, and cool to room temperature. Adjust the concentration of the extract to 0.9%, and adjust the pH value to 4.5 with acetic acid. 5% edible ethanol is added in a mass ratio, and bacteria are removed by suction filtration with a 0.45 μm microporous membrane to obtain sterile tea soup. Pack the aseptic tea soup into 1L glass bottles, inoculate the mother liquid of 5% kombucha fungus by volume and 3% kombucha fungus film by mass, then cover the glass bottle with a breathable sealing film and put it in...

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Abstract

The invention discloses a preparation method of black tea fungus pellicle. The preparation method comprises the following steps of 1, performing extraction on black tea by using water for two times, primarily filtering and then mixing extract liquid acquired by the two times of extraction; 2, adding tannase into the extract liquid, then performing enzymolysis, heating to inactivate enzyme, and cooling to room temperature; 3, adding cane sugar into the extract liquid, then boiling and further cooling to the room temperature; 4, regulating the concentration of the extract liquid to be between 0.5-0.8%, and regulating the pH value of the same to be between 4-5; 5, adding edible ethyl alcohol into the extract liquid, and then filtering to acquire aseptic tea soup; 6, subpackaging the aseptic tea soup into a wide-mouth glass bottle, then inoculating with black tea fungus mother liquid and black tea fungus pellicle, covering with an air permeable sealing film, and then putting the bottle into an incubator for culturing; and 7, when the pellicle in the incubator is mature, cleaning fungus solution with clear water, then sterilizing, and cooling to acquire the finished black tea fungus pellicle. The growth amount of the black tea fungus pellicle is improved, and the black tea fungus solution is clear and good in taste.

Description

technical field [0001] The invention relates to a method for preparing a fungus film, in particular to a method for preparing a fungus fungus film of kombucha. Background technique [0002] Kombucha, also known as Haibao, contains a part of tea extract, live microorganisms and their metabolites. Kombucha inhibits the growth of harmful bacteria, it can clean the stomach, prevent and treat constipation and hemorrhoids, help digestion, prevent and treat cardiovascular diseases such as hypertension, hyperlipidemia, arteriosclerosis and diabetes; and it has the effect of strengthening the body Immunity and other functions. The main microorganisms that play a leading role in the fermentation process of kombucha are acetic acid bacteria, yeast and lactic acid bacteria, forming a symbiotic system of three types of microorganisms. During kombucha fermentation, yeast can quickly convert sugar into ethanol, thereby stimulating the growth of acetic acid bacteria; and the main metaboli...

Claims

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Application Information

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IPC IPC(8): A23F3/16C12P19/04C12R1/02
Inventor 刘盼盼龚自明高士伟郑鹏程滕靖王雪萍王胜鹏叶飞郑琳
Owner INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
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