Application of quaternized natural polymer material as virus vector delivery enhancing material
A technology of natural polymers and viral vectors, which is applied in the application field of quaternized natural polymer materials as viral vector delivery enhancement materials, and can solve the problem of difficult balance between high nucleic acid delivery efficiency and high biocompatibility of viral vectors. Normalize the problems of delivery efficiency and poor biocompatibility, and achieve the effect of improving nucleic acid delivery efficiency and biocompatibility, improving nucleic acid delivery efficiency, and improving delivery efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0019] Example 1
[0020] 1) Add 2.0 g of epichlorohydrin dropwise to 3.0 g of N,N-dimethylethylamine, stir at 50°C for 8 hours, after the end, the ether and n-hexane are precipitated and washed twice, and dried under reduced pressure to obtain the ring Oxygen quaternary ammonium salt A, the molecular formula is
[0021] 2) Add 2.0 g of chitosan citrate (degree of deacetylation: 95%, average molecular weight: 6000) into 200 mL of deionized water, and heat to dissolve at 50°C. Then 2.5 g epoxy quaternary ammonium salt A was dissolved in 20 mL deionized water and added to the chitosan citrate solution, and reacted at 50° C. for 48 hours.
[0022] 3) After the reaction, the quaternized natural polymer delivery material 1 can be obtained by washing three times with ethanol precipitation and drying under reduced pressure.
Example Embodiment
[0023] Example 2
[0024] 1) Add 10g of epichlorohydrin dropwise to 30g of N,N-dimethylpropylamine, stir for 24 hours at 35°C, after the end, petroleum ether and n-hexane are precipitated and washed 3 times, dried under reduced pressure to obtain epoxy quaternary ammonium salt B, the molecular formula is
[0025] 2) Add 3.0 g of chitosan hydrochloride (degree of deacetylation: 90%, average molecular weight: 20000) into 200 mL of deionized water, and heat to dissolve at 45°C. Then 1.0 g epoxy quaternary ammonium salt B was dissolved in 10 mL deionized water and added to the chitosan hydrochloride solution, and reacted at 40°C for 24 hours.
[0026] 3) After the reaction, the quaternized natural polymer delivery material 2 can be obtained by washing twice with ethanol precipitation, dialysis, and freeze drying.
Example Embodiment
[0027] Example 3
[0028] 1) 5.0g of hydroxypropyl cellulose (average molecular weight: 18000) was dissolved in 40mL of N,N-dimethylformamide, 1.5g of phenyl p-nitrochloroformate and 10mL of pyridine were added and stirred at 0°C for 4 hours, Then precipitate 3 times with anhydrous ether.
[0029] 2) Add the purified product from step 1) to 30 mL of N,N-dimethylformamide, add 4.5 g of ethylenediamine, and stir for 24 hours under a nitrogen atmosphere at 25°C.
[0030] 3) Precipitating in ether for 3 times and drying in vacuum to obtain hydroxypropyl cellulose with primary amino groups.
[0031] 4) Dissolve 2.0g aminohydroxypropyl cellulose in 200mL deionized water; 0.4g 3-chloro-2-hydroxypropyltrimethylammonium chloride and 0.4g sodium hydroxide dissolved in 20mL deionized water before adding, React at 30°C for 24 hours.
[0032] 5) After the reaction, the quaternized natural polymer delivery material 3 can be obtained by washing three times with ethanol precipitation and drying under...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap