Preparation and application of label-free odd-even discriminator based on silver nanoclusters and graphene oxide

A silver nano-cluster, odd-even technology, applied in special data processing applications, instruments, logic circuits with logic functions, etc., can solve the problems of high cost, labor-intensive, poor light resistance of dyes, etc.

Inactive Publication Date: 2017-09-19
TAIZHOU UNIV
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, molecular logic devices based on DNA self-assembly have made some progress, but it is still a great challenge to obtain low-cost and high-efficiency DNA molecular logic gates.
Most of the existing DNA fluorescent logic gates need to be labeled with fluorescent dyes. This method has great defects, such as high cost, labor-intensive, and poor light resistance of dyes. Therefore, a label-free molecule based on silver nanoclusters was developed. Logic devices are of great significance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application of label-free odd-even discriminator based on silver nanoclusters and graphene oxide
  • Preparation and application of label-free odd-even discriminator based on silver nanoclusters and graphene oxide
  • Preparation and application of label-free odd-even discriminator based on silver nanoclusters and graphene oxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation of DNA-protected silver nanoclusters: First, the template DNA (Ag-DNA) was dissolved in a sodium phosphate buffer solution (10mM Na 2 HPO 4 / NaH 2 PO 4 ,100mM CH 3 COONa, 5mM Mg(CH 3 COO) 2 , pH 7.5), then heat the buffer solution to 90°C for 10 minutes, and then slowly cool to room temperature. Add 1 mM silver nitrate solution to the buffer system and continue shaking for 30 seconds. After incubating for 30 minutes, add 1 mM NaBH again 4 The solution was shaken vigorously for 1 min. In the final solution, the ratio of template DNA, silver nitrate and sodium borohydride is 1:6:6.

Embodiment 2

[0019] Based on the construction of graphene oxide and silver nanocluster sensing platform: The molecular logic platform is composed of silver nanoclusters protected by 100nM DNA and 15μg / mL graphene oxide mixed for 15min and then assembled. When we convert a decimal number into a binary number commonly used in computer languages, we use 1 and 0 to represent the input and non-input of the sequence. figure 2 , As shown in 4, 6, adding different combinations of input DNA (N 0 -N 4 ), the parity of the decimal numbers from 0 to 31 can be judged.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention belongs to the analytical chemistry optic logic gate technology field, and relates to a label-free, universal and fast-response fluorescent logic device synthetic method and the application thereof in digit odd-even discrimination. The method comprises: employing graphene oxide and silver nanoclusters protected by single stranded DNA as a detection platform, and adding DNAs with different sequences as signal input so as to obtain output of different fluorescence signals. The method combines nanometer materials to complete odd-even discrimination of decimal digits from 0 to 31 on the molecular level. Compared to the prior art, the serial logic device employs the high fluorescence quantum efficiency feature and the label-free label of noble-metal nanoclusters to combine with novel nanometer materials such as the graphene oxide and the like so as to rapidly realize the operation function of the odd-even discriminator and have latent application values at the aspects of disease diagnosis, molecular biology detection and cell imaging.

Description

Technical field [0001] The invention relates to a method for synthesizing a label-free, universal and fast-response parity discriminator and its application in the field of molecular logic. Background technique [0002] Throughout the development history of human society, the invention of the electronic computer has only appeared for 70 years, but it has achieved unparalleled achievements and has led to scientific and technological revolutions again and again. At present, the development of electronic computers is in the microelectronics period characterized by very large scale integrated circuits. Integrated circuits are based on semiconductor silicon and consist of logic circuits. These logic circuits convert all kinds of information into electrical signals for information transmission, processing and storage. All information is a series of binary codes based on "1" and "0", which represent high level and low level respectively. level. The development of this logic circuit c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): H03K19/20G06F19/20C12N15/11
CPCC12N15/11G16B25/00H03K19/20
Inventor 张思奇韩得满
Owner TAIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap