Active peptide embedded nanoparticle and preparation method thereof

A nanoparticle and active peptide technology, which is applied in food forming, animal protein processing, protein food ingredients, etc., can solve the problems that cannot be absorbed in a complete form, and egg white source active peptides are easy to be degraded, so as to promote comprehensive development and utilization , short production cycle, good absorption effect

Inactive Publication Date: 2017-09-22
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to solve the current problem that egg white-derived active peptides are easily degraded in the digestive system of the gastrointestinal tract and cannot be absorbed in a complete form, and provide a nanoparticle for embedding active peptides and its preparation method

Method used

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  • Active peptide embedded nanoparticle and preparation method thereof
  • Active peptide embedded nanoparticle and preparation method thereof
  • Active peptide embedded nanoparticle and preparation method thereof

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Embodiment 1

[0035] The preparation method of the nanoparticle of embedding active peptide provided by the present invention, comprises the following steps:

[0036] Step 1. Preparation of egg white-derived active peptides: Take 200g of egg white protein powder, prepare egg white protein powder raw material liquid according to the mass ratio of material to liquid at 1:10, heat to 85°C for 10 minutes, denature the protein, and then cool to 50°C, adjust the pH value to 10 with 1M sodium hydroxide solution, add 8g of alkaline protease, continue hydrolysis for 100min, inactivate the enzyme in a boiling water bath for 10min, and finally centrifuge, take the supernatant, and pass through successively the molecular weight of 10K, 3K and 1K After filtering with the ultrafiltration membrane, the active peptides with molecular weights <10KDa, 10KDa-3KDa, 3KDa-1KDa and <1KDa four egg white-derived active peptides were obtained, which were freeze-dried respectively to obtain egg white-derived active pe...

Embodiment 2

[0040] The preparation method of the nanoparticle of embedding active peptide provided by the present invention, comprises the following steps:

[0041]Step 1. Preparation of egg white-derived active peptides: Take 400g of egg white protein powder, prepare egg white protein powder raw material liquid according to the mass ratio of material to liquid at 1:50, heat to 90°C for 15 minutes, denature the protein, and then cool to 50°C, adjust the pH value to 9 with 1M sodium hydroxide solution, add 16g of alkaline protease, continue hydrolysis for 200min, inactivate the enzyme in a boiling water bath for 10min, and finally centrifuge, take the supernatant, and pass through successively the molecular weight of 10K, 3K and 1K After filtering with the ultrafiltration membrane, the active peptides with molecular weights <10KDa, 10KDa-3KDa, 3KDa-1KDa and <1KDa four egg white-derived active peptides were obtained, which were freeze-dried respectively to obtain egg white-derived active pep...

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Abstract

The invention provides an active peptide embedded nanoparticle and a preparation method thereof and aims to solve the problem that albumen derived active peptides which are easily degradable in a gastrointestinal digestive system cannot be completely absorbed currently. By adoption of ionic gel, chitosan and sodium tripolyphosphate serve as nano coating materials to realize embedding of albumen derived active peptides, with four different molecular weights, obtained by enzymolysis, and consequently the albumen peptide-chitosan nanoparticle great in embedding performance is obtained. A gap in protection and promotion of biological absorption and utilization of the albumen derived active peptides is filled, a new idea is provided for deep processing in the industry of egg products, and resource integration and maximal reasonable application of the egg products are realized.

Description

technical field [0001] The invention relates to a nano particle and a preparation method thereof, in particular to a nano particle embedding an active peptide and a preparation method thereof. Background technique [0002] In recent years, food-derived active peptides have attracted more and more attention because of their high safety and the ability to provide amino acids for the human body and regulate certain physiological functions. [0003] Eggs have always been favored by people because of their very high nutritional value and protein bioavailability. my country is a big country in egg production and consumption. The production and consumption of eggs has been the world's number one for many years. However, the deep processing of eggs is still dominated by eggshells, and the degree of deep processing of eggs is still relatively low, especially in the development of functional egg products. Can not meet the growing market demand. [0004] Egg white contains more protei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J3/34A23J3/04A23P10/30C12P21/06
CPCA23J3/341A23J3/04A23P10/30C12P21/06
Inventor 刘静波杜志阳丁龙赵颂宁王艺臻谭大伟
Owner JILIN UNIV
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