Preparation method of microbial environmental friendly sewage treatment agent
An environmentally friendly sewage and treatment agent technology, which is applied in biological water/sewage treatment, microorganism-based methods, biochemical equipment and methods, etc.
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Embodiment 1
[0017] A microbial environment-friendly sewage treatment agent, comprising the following components in parts by weight: 78 parts of D61 cation exchange resin, 85 parts of Pichia CGMCC 2.1801, 8 parts of Bacillus megaterium CGMCC 1.223, 10 parts of magnesium sulfate, 7 parts of activated carbon, plant ash 65 servings.
[0018] The preparation method of microbial environment-friendly sewage treatment agent comprises the following steps:
[0019] 1) Activation of D61 cation exchange resin: soak in 35°C deionized water for 10 hours, soak in 3% potassium hydroxide for 3 hours, then wash with water until neutral, then soak in hydrochloric acid for 5-7 hours, and wash with water until pH6.6, ready for use;
[0020] 2) Activation of Pichia pastoris CGMCC 2.1801: Take out Pichia pastoris CGMCC2.1801 from the glycerol cryovial, draw 1ml into 100ml YPD liquid medium on a sterilized ultra-clean workbench for overnight culture, and then the obtained culture medium Inoculate 1% into BMGY ...
Embodiment 2
[0025] A microbial environment-friendly sewage treatment agent, comprising the following components in parts by weight: 80 parts of D61 cation exchange resin, 88 parts of Pichia CGMCC 2.1801, 9 parts of Bacillus megaterium CGMCC 1.223, 9 parts of magnesium sulfate, 8 parts of activated carbon, plant ash 63 copies.
[0026] The preparation method of microbial environment-friendly sewage treatment agent comprises the following steps:
[0027] 1) Activation of D61 cation exchange resin: soak in 45°C deionized water for 5 hours, soak in 5% potassium hydroxide for 4 hours, then wash with water until neutral, then soak in hydrochloric acid for 5-7 hours, wash with water until pH6.6, ready for use;
[0028] 2) Activation of Pichia pastoris CGMCC 2.1801: Take out Pichia pastoris CGMCC2.1801 from the glycerol cryovial, draw 1ml into 100ml YPD liquid medium on a sterilized ultra-clean workbench for overnight culture, and then the obtained culture medium Inoculate 1% into BMGY medium a...
Embodiment 3
[0033] A microbial environment-friendly sewage treatment agent, comprising the following components in parts by weight: 50 parts of D61 cation exchange resin, 50 parts of Pichia pastoris CGMCC 2.1801, 5 parts of Bacillus megaterium CGMCC 1.223, 5 parts of magnesium sulfate, 5 parts of activated carbon, plant ash 50 servings.
[0034] A preparation method of microbial environment-friendly sewage treatment agent, comprising the following steps:
[0035] 1) Activation of D61 cation exchange resin: soak in 40°C deionized water for 7 hours, soak in 4% potassium hydroxide for 4 hours, then wash with water until neutral, then soak in hydrochloric acid for 6 hours, and wash with water until pH6. 6. Standby;
[0036] 2) Activation of Pichia pastoris CGMCC 2.1801: Take out Pichia pastoris CGMCC2.1801 from the glycerin cryovial, draw 1ml into 100ml YPD liquid medium on a sterilized ultra-clean workbench for overnight culture, and then the obtained culture medium Inoculate 1% into BMGY ...
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