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Preparation method of microbial environmental friendly sewage treatment agent

An environmentally friendly sewage and treatment agent technology, which is applied in biological water/sewage treatment, microorganism-based methods, biochemical equipment and methods, etc.

Inactive Publication Date: 2017-09-29
南京爱斯利尔环保设备工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the development of industry, the situation of water pollution continues to deteriorate, and the shortage of water resources has seriously threatened people's lives. Microbiological treatment of sewage is now widely used in industrial sewage, domestic sewage and other fields as an effective method, and only microbial treatment of sewage is used. The method is very limited, the treatment capacity is small, and the effect is slow. There is an urgent need for a bacterial agent that can handle a large amount of sewage and quickly treat sewage, and comprehensively improve the sewage treatment capacity, so that the sewage can meet the basic discharge standards.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A microbial environment-friendly sewage treatment agent, comprising the following components in parts by weight: 78 parts of D61 cation exchange resin, 85 parts of Pichia CGMCC 2.1801, 8 parts of Bacillus megaterium CGMCC 1.223, 10 parts of magnesium sulfate, 7 parts of activated carbon, plant ash 65 servings.

[0018] The preparation method of microbial environment-friendly sewage treatment agent comprises the following steps:

[0019] 1) Activation of D61 cation exchange resin: soak in 35°C deionized water for 10 hours, soak in 3% potassium hydroxide for 3 hours, then wash with water until neutral, then soak in hydrochloric acid for 5-7 hours, and wash with water until pH6.6, ready for use;

[0020] 2) Activation of Pichia pastoris CGMCC 2.1801: Take out Pichia pastoris CGMCC2.1801 from the glycerol cryovial, draw 1ml into 100ml YPD liquid medium on a sterilized ultra-clean workbench for overnight culture, and then the obtained culture medium Inoculate 1% into BMGY ...

Embodiment 2

[0025] A microbial environment-friendly sewage treatment agent, comprising the following components in parts by weight: 80 parts of D61 cation exchange resin, 88 parts of Pichia CGMCC 2.1801, 9 parts of Bacillus megaterium CGMCC 1.223, 9 parts of magnesium sulfate, 8 parts of activated carbon, plant ash 63 copies.

[0026] The preparation method of microbial environment-friendly sewage treatment agent comprises the following steps:

[0027] 1) Activation of D61 cation exchange resin: soak in 45°C deionized water for 5 hours, soak in 5% potassium hydroxide for 4 hours, then wash with water until neutral, then soak in hydrochloric acid for 5-7 hours, wash with water until pH6.6, ready for use;

[0028] 2) Activation of Pichia pastoris CGMCC 2.1801: Take out Pichia pastoris CGMCC2.1801 from the glycerol cryovial, draw 1ml into 100ml YPD liquid medium on a sterilized ultra-clean workbench for overnight culture, and then the obtained culture medium Inoculate 1% into BMGY medium a...

Embodiment 3

[0033] A microbial environment-friendly sewage treatment agent, comprising the following components in parts by weight: 50 parts of D61 cation exchange resin, 50 parts of Pichia pastoris CGMCC 2.1801, 5 parts of Bacillus megaterium CGMCC 1.223, 5 parts of magnesium sulfate, 5 parts of activated carbon, plant ash 50 servings.

[0034] A preparation method of microbial environment-friendly sewage treatment agent, comprising the following steps:

[0035] 1) Activation of D61 cation exchange resin: soak in 40°C deionized water for 7 hours, soak in 4% potassium hydroxide for 4 hours, then wash with water until neutral, then soak in hydrochloric acid for 6 hours, and wash with water until pH6. 6. Standby;

[0036] 2) Activation of Pichia pastoris CGMCC 2.1801: Take out Pichia pastoris CGMCC2.1801 from the glycerin cryovial, draw 1ml into 100ml YPD liquid medium on a sterilized ultra-clean workbench for overnight culture, and then the obtained culture medium Inoculate 1% into BMGY ...

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PUM

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Abstract

The invention discloses a preparation method of microbial environment-friendly sewage treatment agent, comprising the following steps: activation of D61 cation exchange resin; activation of Pichia pastoris CGMCC 2.1801; activation of Bacillus megaterium CGMCC 1.223; activation of Pichia pastoris CGMCC 2.1801 and activated Bacillus megaterium CGMCC 1.223 were inoculated into the mixed strain medium for mixed culture to obtain a mixed bacterial solution; the mixed bacterial solution was mixed with activated D61 cation exchange resin, magnesium sulfate, activated carbon and plant ash to obtain a microbial environmental sewage treatment agent. Through repeated research and practice by the applicant, the present invention finds that the microbial environment-friendly sewage treatment agent of the present application can be directly used for sewage treatment, especially for the removal of nitrogen, phosphorus and potassium in industrial sewage, wherein the removal rate of nitrogen can reach about 96%, and the removal rate of phosphorus can reach about 96%. The removal rate can reach about 85%, and the removal rate of potassium can reach about 88%.

Description

technical field [0001] The invention belongs to the field of sewage treatment, in particular to a preparation method of a microbial environment-friendly sewage treatment agent. Background technique [0002] With the development of industry, the situation of water pollution continues to deteriorate, and the shortage of water resources has seriously threatened people's lives. Microbiological treatment of sewage is now widely used in industrial sewage, domestic sewage and other fields as an effective method, and only microbial treatment of sewage is used. The methods are very limited, the treatment capacity is small, and the effect is slow. There is an urgent need for a bacterial agent that can treat a large amount of sewage and can quickly treat sewage, and comprehensively improve the sewage treatment capacity, so that the sewage can meet the basic discharge standards. Contents of the invention [0003] Purpose of the invention: the purpose of the invention is to provide a p...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C12N1/16C12R1/11C12R1/84C02F101/10C02F101/16C02F101/20
CPCC02F3/347C02F3/34C02F2101/105C02F2101/16C02F2101/20C12N1/16C12N1/20
Inventor 周金超
Owner 南京爱斯利尔环保设备工程有限公司
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