A method for recovering cellulase in solution
A technology for cellulase and solution recovery, applied in biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of high cost and low recovery efficiency, and achieve the effects of short time consumption, simple operation, and comprehensive utilization
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Embodiment 1
[0031] Add 5 parts by mass of TCSL-N25 to a solution of 5000 parts by mass of cellulase protein concentration of 200 mg / L (pure water configuration, pH=7.0), adjust the pH of the solution to 3.0 with hydrochloric acid to make TCSL-N25 and fiber The cellulase was precipitated out, and the cellulase was recovered by solid-liquid separation. Add the recovered cellulase to 5000 parts by mass of 50mM acetic acid-sodium acetate buffer, add 100 parts by mass of microcrystalline cellulose, enzymolyze at 50°C for 24 hours, and measure the concentration of the enzymolyzed solution by a biosensing analyzer. Glucose content, statistical results are shown in Table 1.
Embodiment 2
[0033] Add 25 parts by mass of ASL to a solution of 5000 parts by mass of cellulase protein concentration of 400 mg / L (pure water configuration, pH=7.0), adjust the pH of the solution to 3.2 with hydrochloric acid to precipitate ASL and cellulase , the cellulase was recovered by centrifugation. Add the recovered cellulase to 5000 parts by mass of 50mM acetic acid-sodium acetate buffer, add 100 parts by mass of microcrystalline cellulose, enzymolyze at 50°C for 24 hours, and measure the concentration of the enzymolyzed solution by a biosensing analyzer. Glucose content, statistical results are shown in Table 1.
Embodiment 3
[0035] Add 5 parts by mass of TCSL-N25 to a solution of 5000 parts by mass of cellulase protein concentration of 1000 mg / L (configured in acetic acid-sodium acetate buffer solution, pH=4.8, 25mM), adjust the pH of the solution to 2.8 with hydrochloric acid TCSL-N25 and cellulase were precipitated, and the cellulase was recovered by centrifugation. Add the recovered cellulase to 5000 parts by mass of 50mM acetic acid-sodium acetate buffer, add 100 parts by mass of microcrystalline cellulose, enzymolyze at 50°C for 24 hours, and measure the concentration of the enzymolyzed solution by a biosensing analyzer. Glucose content, statistical results are shown in Table 1.
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