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Method for accelerating induction of germination of epipremnum aureum

A green radish and budding technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of susceptible pathogens, large-scale stem rot, economic loss of green radish, shorten the growth cycle, accelerate the induction Effect

Inactive Publication Date: 2017-10-03
东方上彩现代农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The propagation of pothos is currently carried out by cuttings. Due to the high temperature and high humidity in the growing environment of pothos, the offspring of cuttings are very susceptible to pathogens. Large-scale stem rot and root rot often occur in production, causing certain economic losses to the cultivation of pothos

Method used

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  • Method for accelerating induction of germination of epipremnum aureum
  • Method for accelerating induction of germination of epipremnum aureum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0133] The explants of the pothos were cultivated according to the following method and culture solution:

[0134] Step 1, select the mother plant and use 80% carbendazim 1000 times solution to irrigate the roots for 30 days, once every 10 days;

[0135] Step 2, taking the stem segment at the growth point of the mother plant;

[0136] Step 3, the stem section is sterilized to obtain the explant of the stem section of Potula radish;

[0137] Step 4, after pruning the explant of the stem section of the pothos, to obtain a section sample of the stem section;

[0138] Step 5, soaking the stem segment sample with 2g / L polyvinylpyrrolidone for 10-15 minutes;

[0139] Step 6, inoculate the stem segment sample soaked in the drug into callus induction medium No. 1, and carry out dark culture for 10 days at a temperature of 25°C-28°C and a humidity of 50%-65%, to obtain the first callus;

[0140] Step 7, transferring the first callus into induction medium No. 2, and culturing at a t...

Embodiment 2

[0170] The explants of the pothos were cultivated according to the following method and culture solution:

[0171] Step 1, select the mother plant and use 80% carbendazim 1000 times solution to irrigate the roots for 30 days, once every 10 days;

[0172] Step 2, taking the stem segment at the growth point of the mother plant;

[0173] Step 3, the stem section is sterilized to obtain the explant of the stem section of Potula radish;

[0174] Step 4, after pruning the explant of the stem section of the pothos, to obtain a section sample of the stem section;

[0175] Step 5, soaking the stem segment sample with 2g / L polyvinylpyrrolidone for 10-15 minutes;

[0176] Step 6, inoculate the stem segment sample soaked in the drug into callus induction medium No. 1, and carry out dark culture for 10 days at a temperature of 25°C-28°C and a humidity of 50%-65%, to obtain the first callus;

[0177] Step 7, transferring the first callus into induction medium No. 2, and culturing at a t...

Embodiment 3

[0207] The explants of the pothos were cultivated according to the following method and culture solution:

[0208] Step 1, select the mother plant and use 80% carbendazim 1000 times solution to irrigate the roots for 30 days, once every 10 days;

[0209] Step 2, taking the stem segment at the growth point of the mother plant;

[0210] Step 3, the stem section is sterilized to obtain the explant of the stem section of Potula radish;

[0211] Step 4, after pruning the explant of the stem section of the pothos, to obtain a section sample of the stem section;

[0212] Step 5, soaking the stem segment sample with 2g / L polyvinylpyrrolidone for 10-15 minutes;

[0213] Step 6, inoculate the stem segment sample soaked in the drug into callus induction medium No. 1, and carry out dark culture for 10 days at a temperature of 25°C-28°C and a humidity of 50%-65%, to obtain the first callus;

[0214] Step 7, transferring the first callus into induction medium No. 2, and culturing at a t...

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Abstract

The invention provides a method for accelerating induction of germination of epipremnum aureum. The method comprises the following steps: selecting a stock plant, pretreating the stock plant, and then taking an epipremnum aureum stem segment as an explant; soaking the epipremnum aureum stem segment explant in a drug, and inoculating the explant to a callus inducing medium to obtain calluses; transferring the calluses to a subculture medium for culture to obtain second generation calluses; inoculating the second generation calluses to a bud induction medium for culture to obtain epipremnum aureum multiple shoots. According to the method provided by the invention, aseptic seedlings of the epipremnum aureum stem segment is cultured by an epipremnum aureum tissue culture technology, the induction of the germination of the epipremnum aureum can be accelerated, the growth cycle can be shortened, the losses caused by large-scale stem rot and root rot caused by adverse external factors can be avoided or reduced, and the problems that at present, by virtue of cutting propagation of the epipremnum aureum, subsequent generations of cutting propagation are extremely susceptible to pathogens, large-scale stem rot and root rot phenomena occur frequently in production, and certain economic losses are caused to the culture of the epipremnum aureum are avoided.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a method for accelerating the induction of sprouting of pothos. Background technique [0002] Green dill (scientific name: Epipremnumaureum), belonging to the genus Eucheuma, is a large evergreen vine that grows in tropical regions. It often climbs and grows on rocks and tree trunks in the rainforest. The petioles on mature branches are thick, 30-40 cm long, the base is slightly enlarged, the upper joints are 2.5-3 cm long, slightly hypertrophic, the ventral surface is wide grooved, the leaf sheath is long, the leaves are thin leathery, emerald green, usually (especially the leaves) There are many irregular pure yellow plaques, entire margins, ovate or ovate-oblong on unequal sides, short and acuminate at the apex, deeply cordate at the base, slightly thicker, and slightly raised on both sides. [0003] The origin of pothos is in the tropical rainforest of the Solomon ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王健刘坤申亮卢迪浩郑德助施金玉包宇飞
Owner 东方上彩现代农业有限公司
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