Tissue culture method for fast propagation of new Lilium oriental hybrid variety

Abstract

The invention discloses a tissue culture method for fast propagation of a new Lilium oriental hybrid variety, and in the method, a low concentration hormone formula test-tube plantlet breeding method is used for fast propagation, and river sand or silt are used as a matrix for hardening seedling culture. The method particularly includes the following steps: selecting plants of the new Lilium oriental hybrid variety for virus detection, and taking a bulb of an identified virus-free plant as an explant for explant sterilization; respectively performing primary culture, subculture and rooting culture on the sterilized explants; performing low temperature treatment before hardening seedling; selecting the river sand or the silt as the matrix for hardening seedling; and after completion of the hardening seedling, transplanting the bulb to a high altitude field for bulb seeding. According to the tissue culture method, a large number of high-quality and stable Lilium oriental seedlings can be obtained, and the shortcomings in the prior art can be overcome.

Description

technical field [0001] The invention relates to the technical field of rapid breeding of new hybrid varieties of oriental lily, in particular to a tissue culture method for rapid breeding of new hybrid varieties of oriental lily. Background technique [0002] Lily (Lilium) is the general term for all species of the genus Lilium. Its flowers are large, colorful, and unique in shape. It is one of the world's famous flowers and a high-end precious flower that is well-known in the global market. It is widely used in gardens, potted flowers, especially fresh cut flowers, and is deeply loved by people. Westerners have always regarded it as a symbol of holiness. China has had the custom of using lilies to represent purity and joy since ancient times. In China, lilies are high-end cut flowers, and their sales prices are significantly higher than other cut flower varieties, especially Oriental lily (O) and OT type lily, which are more popular with consumers because of their strong f...

AI Technical Summary

Problems solved by technology

Due to the long-term and large amount of foreign imports costing hundreds of millions of dollars in foreign exchange every year, not only the cost of bulbs is high, but some varieties are also of poor quality due to the difficulty in introducing seed sources, and the species are degraded, which cannot meet the requirements for export to participate in market competition.

In addition, the procedures for importing bulbs are complicated, and it is necessary to purchase seeds and make an appointment one year in advance, resulting in blind purchase of seeds, planting for multiple generations without changing species, severe degradation of bulbs, resulting in disordered varieties, reduced quality, and serious pests and diseases, which seriously restricts my country's lily industry. normal development of