Tissue culture method for fast propagation of new Lilium oriental hybrid variety
A new variety, lily technology, applied in the field of rapid breeding control of new oriental lily hybrid varieties, can solve the problems of bulb degradation, high cost of bulbs, complicated procedures for importing bulbs, etc.
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[0022] The technical scheme of the present embodiment comprises the steps:
[0023] 1) Sterilization and cultivation of exogenous bodies: For the self-cultivated new oriental lily hybrid varieties Zhuzhouhong, Huaxianzi, Pleasant Goat, and Lorna as materials, select 5-10 strains for each variety for virus detection, and identify virus-free plants, Take its bulbs as explants. Sterilization of explants: peel off scales, rinse with tap water, dry, then soak with 75% alcohol for 30 seconds, then disinfect with 0.1% mercury liter for 15-20 minutes, wash with sterile water 3-4 times. The formula of the primary medium is: MS+0.3~0.5mg. / L 6-BA, 3% sucrose;
[0024] 2) Subculture: peel off the scales at the base of the test-tube plantlets and inoculate them on the subculture medium. The formula for subculture for 2-5 generations is: MS+0.2-0.3 mg. / L 6-BA, 3% sucrose; Properly adjust the formula according to the germination situation, use 0.2mg. / L6-BA for more germination, and 0.3mg. / ...
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