Tissue culture method for fast propagation of new Lilium oriental hybrid variety
A new variety, lily technology, applied in the field of rapid breeding control of new oriental lily hybrid varieties, can solve the problems of bulb degradation, high cost of bulbs, complicated procedures for importing bulbs, etc.
Inactive Publication Date: 2017-10-20
ZHUZHOU INST OF AGRI SCI +1
6 Cites 1 Cited by
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Abstract
The invention discloses a tissue culture method for fast propagation of a new Lilium oriental hybrid variety, and in the method, a low concentration hormone formula test-tube plantlet breeding method is used for fast propagation, and river sand or silt are used as a matrix for hardening seedling culture. The method particularly includes the following steps: selecting plants of the new Lilium oriental hybrid variety for virus detection, and taking a bulb of an identified virus-free plant as an explant for explant sterilization; respectively performing primary culture, subculture and rooting culture on the sterilized explants; performing low temperature treatment before hardening seedling; selecting the river sand or the silt as the matrix for hardening seedling; and after completion of the hardening seedling, transplanting the bulb to a high altitude field for bulb seeding. According to the tissue culture method, a large number of high-quality and stable Lilium oriental seedlings can be obtained, and the shortcomings in the prior art can be overcome.
Application Domain
Plant tissue cultureHorticulture methods
Technology Topic
River sandVirus detection +10
Examples
- Experimental program(1)
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