A kind of preparation method of core-shell structure

Abstract

The invention relates to a preparation method of a core-shell structure, namely, a biological brick for biological printing (such as 3D biological printing) and tissue engineering. The method comprises the steps as follows: a) providing a hydrophobic surface, preferably, a super-hydrophobic surface; b) dropping a core material solution to the hydrophobic surface in step a) to form a droplet so as to obtain a core-shell structure on the innermost side; c) optionally, dropping the core material solution to the formed droplet surface to obtain another core-shell structure; d) optionally, dropping a wall material solution to the formed droplet surface to obtain a shell structure; e) optionally, repeating step c) and / or step d) one or more times or alternately performing step c) and step d) one or more times; f) dropping the wall material solution to the formed droplet surface to obtain a shell structure on the outermost side, wherein the core material or wall material contained in each core or shell is selected independently.

Description

technical field [0001] The invention relates to technical fields such as biomedicine, material science, bioprinting (such as 3D bioprinting), tissue engineering and the like. In particular, the present invention relates to a preparation method of a core-shell structure, which can be used for delivering active substances, or for bioprinting (such as 3D bioprinting) and tissue engineering. Background technique [0002] The core-shell structure refers to the use of film-forming materials to encapsulate solids, liquids or gases in them to form tiny containers with a diameter of tens of microns to thousands of microns. The material used to form the core layer is called the core material (or capsule) Heart material), the material used to form the shell (membrane) of the outer capsule is called wall material (or called encapsulation material). [0003] At present, the main preparation methods of core-shell structure can be divided into chemical method, physical method and physicoc...

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Problems solved by technology

In order to make the droplet diameter smaller, it is usually necessary to apply a higher vibration frequency and amplitude, which will also greatly affect the cell activity, making it difficult to take into account the cell droplet size and cell activity

[0009] (3) The size control of core and shell is not accurate

The existing preparation methods and devices can only rely on the spontaneous chemical reaction of the core and the shell to form the shape, and cannot precisely control the final core-shell structure size, and cannot control the respective thicknesses of the core and shell. The repeatability is poor, that is, it is difficult to realize the core, shell On-demand individual control and adjustment of shell dimensions

[0010] (4) In the process of using temperature-sensitive materials to prepare the core-shell structure, it is difficult to ensure the accuracy of the multiple and repeated titration process due to the usual manual dripping method. Low, large dimensional error, and difficult to fabricate multilayer shells, especially core-shell structures with multiple shells of different thicknesses

[0011] (6) When the core-shell structure is prepared by the sharp hole method, for some temperature-sensitive materials mainly containing polar molecules, the formation process of the droplet and the wrapping efficiency of the fractional titration cannot be guaranteed during the preparation, which limits the high efficiency of automation preparation of

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