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Gelatin-polylactic acid grafted amphipathic copolymer nano-micelle material and preparation method thereof

A technology of amphiphilic copolymer and graft copolymer, which is applied in the field of gelatin-polylactic acid grafted amphiphilic copolymer nanomicelle material and its preparation, can solve problems such as damage to biological cells, and achieves reduction of dosage, reduction of damage, The effect of reducing the positive charge

Inactive Publication Date: 2017-10-20
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, usually medical coagulation needs to use a large amount of gelatin, which contains more positively charged groups, such as amino (-NH 2 ), while positive charges have certain damage to biological cells

Method used

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  • Gelatin-polylactic acid grafted amphipathic copolymer nano-micelle material and preparation method thereof
  • Gelatin-polylactic acid grafted amphipathic copolymer nano-micelle material and preparation method thereof
  • Gelatin-polylactic acid grafted amphipathic copolymer nano-micelle material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take gelatin (the number average molecular weight is 1.2×10 4 ) 2g, lactide 8g (feeding ratio is 1:4) in a three-necked flask, vacuum for one hour, add 40mL dimethyl sulfoxide to dissolve, and ensure the nitrogen environment, heat the oil bath to 85 ° C, wait for the raw materials to dissolve After completion, add 40 μL of stannous octoate, and stir and react at 85°C for 24 hours under nitrogen protection to obtain a gelatin-polylactic acid graft copolymer reaction solution; the reaction solution is precipitated with distilled water to remove reactants that do not participate in the reaction, polylactic acid homogeneous polymer, the above solution was transferred to a dialysis bag for dialysis for 3 days, and after the dialysis was completed, it was freeze-dried to obtain a solid product.

[0036] TEM image of Gel-g-PLLA nanomicelle ( figure 1 shown), showing that the nanomicelles are uniformly spherical.

[0037] 1 HNMR spectrum ( figure 2 Shown) is a liquid nucle...

Embodiment 2

[0043] Take gelatin (the number average molecular weight is 1.2×10 4 ) 2g, lactide 16g (feeding ratio: 1:8) in a three-necked flask, vacuumize for one hour, add 40mL dimethyl sulfoxide to dissolve, and ensure the nitrogen environment, heat the oil bath to 85°C, and wait for the raw materials to dissolve After completion, add 72 μL of stannous octoate, and stir and react at 85°C for 24 hours under nitrogen protection to obtain a gelatin-polylactic acid graft copolymer reaction solution; the reaction solution is precipitated with distilled water to remove reactants that do not participate in the reaction, polylactic acid homogeneous polymer, the above solution was transferred to a dialysis bag for dialysis for 3 days, and after the dialysis was completed, it was freeze-dried to obtain a solid product.

Embodiment 3

[0045] Take gelatin (the number average molecular weight is 1.2×10 4 ) 2g, lactide 20g (feeding ratio is 1:10) in a three-necked flask, vacuumize for one hour, add 40mL dimethyl sulfoxide to dissolve, and ensure the nitrogen environment, heat the oil bath to 85°C, and wait for the raw materials to dissolve After completion, add 88 μL of stannous octoate, and stir and react at 85°C for 24 hours under nitrogen protection to obtain a gelatin-polylactic acid graft copolymer reaction solution; the reaction solution is precipitated with distilled water to remove unreacted reactants, polylactic acid, and polymer, the above solution was transferred to a dialysis bag for dialysis for 3 days, and after the dialysis was completed, it was freeze-dried to obtain a solid product.

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Abstract

The invention belongs to the technical field of biomedical materials and particularly relates to a gelatin-polylactic acid grafted amphipathic copolymer nano-micelle material and a preparation method of the material. A copolymer nano-micelle is formed by self-assembly of hydrophilic gelatin and hydrophobic polylactic acid grafted materials. The preparation method comprises the steps of dissolving purified gelatin in anhydrous dimethyl sulfoxide, adding monomers such as lactide and a stannous octoate catalyst for heating reaction under the shield of nitrogen to form a gelatin grafted polylactic acid copolymer, and dissolving the polymer in water to form the nano-micelle. The prepared material can form the uniform and stable micelle in the water; the micelle is 100-500nm in particle size; the surface of the micelle is positively charged; when a concentration of the micelle in blood is 0.1-5mg.mL<-1>, blood coagulation can be accomplished within 2-5min; and the material can serve as a blood coagulation material with excellent performance and also has broad application prospects in antibacterial spray and entrapment drugs.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a gelatin-polylactic acid grafted amphiphilic copolymer nano-micelle material and a preparation method thereof. Background technique [0002] Amphiphilic polymers refer to polymers in which both hydrophilic and hydrophobic segments exist in the polymer structure. This property of having two different hydrophilic segments at the same time enables amphiphilic polymers to self-assemble into a variety of amphiphilic polymers with different shapes through a thermodynamic stabilization process in a specific solvent (water or organic phase solvent). nanomicelle. Due to its unique and stable structure under the action of a thermodynamic system, amphiphilic polymer nanomicelles also show many unique characteristics in use and function, and have broad application prospects in the field of biomedical materials. [0003] Polylactic acid is a polymer material synthe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G63/08C08H1/00A61L24/10A61L24/00
CPCA61L24/001A61L24/0015A61L24/0026A61L24/104A61L2300/404C08G63/08C08H1/00C08L89/00
Inventor 范仲勇杜焙焙胡艳飞马瑞嘉王杰林
Owner FUDAN UNIV
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