A heat-resistant freeze-drying protective agent of swine fever live vaccine

A swine fever live vaccine and heat-resistant freeze-drying technology are applied in the field of heat-resistant freeze-drying protective agents, which can solve problems such as energy waste and cost increase, and achieve the effects of reducing side reactions, preventing denaturation and protecting activity

Active Publication Date: 2019-10-29
商丘美兰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Stricter storage conditions not only lead to increased costs and waste of energy, but in actual production applications, there are also examples of swine fever caused by improper storage and transportation of vaccines from time to time, resulting in the occurrence of swine fever

Method used

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  • A heat-resistant freeze-drying protective agent of swine fever live vaccine
  • A heat-resistant freeze-drying protective agent of swine fever live vaccine
  • A heat-resistant freeze-drying protective agent of swine fever live vaccine

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Embodiment 1, a kind of heat-resistant lyoprotectant of swine fever live vaccine is characterized in that: it is formulated by the raw material of following parts by weight: 2% inositol, 1% pendant calendula alcohol, 10 % sucrose, 1% gum arabic, 0.15% sarcosine, 0.05% vitamin C, and the balance is water for injection.

[0014] The preparation method of heat-resistant lyoprotectant of the present invention is as follows:

[0015] Step 1) Dissolve inositol, calendula alcohol, sucrose, and gum arabic in 800ml of water for injection in proportion, and sterilize at 100°C for 30 minutes to obtain solution 1, which is set aside;

[0016] Step 2) Dissolve sarcosine and vitamin C in 100ml of water for injection, filter through a 0.22um filter membrane to obtain solution 2, and set aside;

[0017] Step 3) Combine solution 1 and solution 2, and add water for injection to 1000ml, which is the heat-resistant lyoprotectant of the present invention.

Embodiment 2

[0018] Embodiment 2, the application of heat-resistant lyoprotectant

[0019] (1) Mix the above-mentioned heat-resistant lyoprotectant with the suspension of classical swine fever virus at a ratio of 1:1, dispense quantitatively, and freeze-dry to make a live vaccine for swine fever.

[0020] (2) At the same time, a commercially available swine fever live vaccine (rabbit source) of a certain brand was selected as a control vaccine.

[0021] (3) Vaccine inspection

[0022] The above-mentioned commercially available swine fever live vaccine of a certain brand and the protective agent vaccine of the present invention were stored in a cold storage at 2-8°C, respectively, in 0 months, January, March, June, December, and 18 months, according to the 2015 version of veterinary medicine Code-related items are inspected, and the inspection results are inspected. In order to be closer to actual production, the two vaccines were placed in a 22°C incubator for 7 days to carry out relevan...

Embodiment 3

[0030] Embodiment 3, a kind of heat-resistant lyoprotectant of swine fever live vaccine is characterized in that: it is formulated by the raw material of following parts by weight: 1% inositol, 0.5% pendant calendula alcohol, 5% % sucrose, 0.5% gum arabic, 0.05% sarcosine, 0.01% vitamin C, and the balance is water for injection.

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Abstract

The invention belongs to the technical field of vaccines and particularly relates to a heat-proof freeze-drying protective agent for a live vaccine for hog cholera. The heat-proof freeze-drying protective agent is prepared from raw materials in percentage by weight as follows: 1%-3% of inositol, 0.5%-2% of adonitol, 5%-15% of sucrose, 0.5%-2% of arabic gum, 0.05%-0.2% of sarcosine, 0.01%-0.1% of vitamin C and the balance of injection water. The activity of hog cholera antigen can be effectively protected, the freeze-drying protective agent has a heat-proof protection function, can be used for cold storage and transportation, has good reconstitution properties and does not contain allergic ingredients such as protein, gelatin and the like, side reactions of the vaccine are effectively reduced, resources of raw materials are wide, the preparation method is simple, and the freeze-drying protective agent is low in comprehensive cost and easy to popularize.

Description

technical field [0001] The invention belongs to the technical field of vaccines, in particular to a heat-resistant freeze-drying protective agent of live swine fever vaccine. Background technique [0002] Swine fever is the most important disease that endangers the pig industry, and the use of live swine fever vaccines can effectively prevent the occurrence of the disease. The attenuated swine fever vaccine C strain developed in my country is the most ideal swine fever vaccine recognized in the world. Most of the vaccines are freeze-dried products, which require strict storage and transportation conditions. Generally, they need to be stored at -15°C, and can only be stored for 6 months at 2-8°C. Stricter storage conditions not only lead to cost increase and energy waste, but also in actual production and application, there are also examples of swine fever caused by improper storage and transportation of vaccines from time to time, resulting in the occurrence of swine fever....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/187A61K47/10A61K47/26A61K47/36A61K47/18A61K47/22A61P31/14
CPCA61K39/12A61K47/10A61K47/183A61K47/22A61K47/26A61K47/36A61K2039/5254A61K2039/552C12N2770/24334
Inventor 李自波乔云龙王彦辉姬星宇焦金英姚奋卯胡玉胜郭平霞李国辉
Owner 商丘美兰生物工程有限公司
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