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Compound with autoantibody or similar anti-autoantibody antibody and preparation method of compound
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A technology of autoantibodies and analogs, applied in the field of biomedicine, can solve the problems of short circulation half-life, lack of Fc segment, strong penetrability, etc., and achieve strong specific effects
Pending Publication Date: 2017-10-24
FUDAN UNIV
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Moreover, most of the antibody fragments prepared by this technology lack the Fc segment, have strong penetrability, short half-life in vivo circulation and low immunogenicity, and are easy to carry radionuclides or cytotoxic drugs for guided diagnosis and treatment
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Embodiment 1
[0091] Example 1 Using phage library to screen insulin antibodies
[0092] Coat with insulinantigen (10ug) dissolved in 100μL PBS, overnight at 4°C, wash with PBS and block non-specific binding sites with 3% MPBS, wash at 37°C for 1h, add 100μL of PBS to wash with a titer of 10 12 Phage antibody, incubated at 37°C, washed with PBST and added to logarithmic growth phase TG1, incubated at 37°C, added 800 μL triethylamine, transferred the eluate to a new test tube, and immediately Tris-Hcl (PH7.4) Neutralize and mix well, combine TG1, eluate and TG1 into one tube, incubate at 37°C, add glucose at a final concentration of 2% and ampicillin at 100ug / μL, shake at 37°C for 1 hour, add helper phage, and set at 37°C Incubate for 30 min, add resistance, amplify overnight on a shaker at 30°C, recover the above bacteria for the second round of screening, repeat 4 rounds (experimental steps are as follows: figure 1 shown).
Embodiment 2
[0093] Example 2 Obtain spleenRNA by immunizing ICR mice with IgG from NOD mice
[0094] (1) Immunization of mice: Dissolve the purified NOD serum IgG in PBS solution (2mg / mL), accompanied by an equal volume of adjuvant, and randomly select a number of 6-week-old female ICR mice, a total of three groups, the first, The second group was inoculated with NOD and ICR 200ug / rat respectively, and the third group (negative control) was injected with PBS solution 0.2mL / rat, blood was collected every week to prepare serum samples and stored frozen, and booster immunization was performed every 14 days;
[0095] (2) Determination of immune titer: Coat antigen (50ug / well) on a 96-well plate, overnight at 4°C, block with PBST+1%BSA solution at 37°C for 1 hour, wash with PBST solution for 3 times, and add the above three groups Serum samples (diluted with a dilution ratio of 10, n=3), incubated at 37°C for 1 hour, washed 3 times with PBST solution, added goat anti-mouse HRP-labeled monoclo...
Embodiment 3
[0097] Example 3 Identification of vector pComb3 by single enzymedigestion
[0098] Digest the pComb3 vector with Sac I, Xba I, Xho I, Spe I, Nhe I, and Not I respectively to confirm the correctness of the vector sequence (such as Figure 8 shown).
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Abstract
The invention belongs to the technical field of biomedicines, and relates to a novel method for preparing an autoantigen and autoantibody specific combination, in particular to preparation methods of an autoantibody and an analog thereof, methods for preparing anti-autoantibodyantibody by taking the autoantibody or the analog thereof as an antigen and an analog of the anti-autoantibody antibody, and preparation methods of an anti-human insulin autoantibody and an anti-insulin autoantibody antibody and analogs thereof. The invention provides a preparation method of a peptides compound for preparing a clonal autoantibody or an anti-autoantibody antibody and evaluation of an effect of the binding capacity between the prepared autoantibody specific combination and the autoantibody. An immune test shows that the autoantibody or anti-autoantibody antibody specific combination prepared through the preparation method disclosed by the invention has a physicochemical characteristic of a monoclonal antibody, and the anti-autoantibody antibody specific combination can be effectively combined with the autoantibody.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a specific conjugate of an autoantigen and an autoantibody and a preparation method thereof. It specifically relates to a method for preparing an autoantigen-specific binding substance and using the prepared specific binding substance as an antigen to screen to obtain a compound or antibody analog with anti-autoantibody antibody characteristics and a method thereof. Background technique [0002] The existing technology discloses the status quo of autoimmune diseases. In 1904, Donatht and Landsteiner first detected antibodies in the patient's urine that caused the destruction of red blood cells in a cold environment, and proposed the concept of "autoantibodies". The prologue of AID research. Autoimmune diseases are caused by the breakdown of the body's immune tolerance to self-antigens, resulting in the activation of self-reactive immune cells and the production of autoantibodi...
Claims
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