A two-phase method for rapid screening of denitrifying phosphorus-accumulating bacteria

A denitrifying phosphorus-accumulating bacteria and fast technology, applied in the field of environmental microorganisms, can solve the problems of heavy workload and long time consumption, and achieve the effects of reducing workload, improving selectivity, and shortening the screening cycle

Inactive Publication Date: 2020-06-19
WUHAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the existing denitrifying phosphorus accumulating bacteria enrichment and screening methods, such as large workload and long time consumption, and provide a two-phase method for quickly screening denitrifying phosphorus accumulating bacteria

Method used

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  • A two-phase method for rapid screening of denitrifying phosphorus-accumulating bacteria
  • A two-phase method for rapid screening of denitrifying phosphorus-accumulating bacteria
  • A two-phase method for rapid screening of denitrifying phosphorus-accumulating bacteria

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Embodiment 1

[0021] The first step: pretreatment of activated sludge

[0022] Take 20ml of activated sludge into a 250ml Erlenmeyer flask, add 150ml of distilled water, shake at 110rpm at 30°C for 24 hours, then centrifuge at 4000rpm, discard the supernatant by decantation to obtain a mixture.

[0023] The second step: anaerobic phase constant temperature culture

[0024] Put the mixture in a 250ml Erlenmeyer flask, add 150ml of anaerobic phase containing additional carbon source phosphorus-free medium, pass through nitrogen for 10min, quickly seal the bottle mouth with a parafilm, place at 110rpm, 30°C and shake for 24h (DO<0.1mg / L). After the anaerobic culture was completed, water samples were taken to detect the total phosphorus content.

[0025] Wherein, the anaerobic phase contains the additional carbon source phosphorus-free medium including the following components:

[0026] 2g / L CH 3 COONa; 0.15g / L NH 4 Cl;

[0027] 0.2g / L MgSO 4 ;0.015g / L CaCl 2 ;

[0028] 0.1g / L NaCl; 1m...

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Abstract

The invention discloses a method for rapid screening of denitrifying phosphorus accumulating bacteria by a two-phase process. According to the method, by simulation of a living environment of the denitrifying phosphorus accumulating bacteria, anaerobic-anoxic two-phase alternate conditions are designed, a phosphorus-free culture medium containing an external carbon source at an anaerobic phase and a phosphorus-free culture medium free of external carbon sources at an anoxic phase are adopted respectively for screening, a domestication culture medium is diluted and then coats screened single strains, the screened single strains are respectively dibbled in a high-phosphorous and low-phosphorous solid medium containing 5-bromo-4-chloro-3-indolyl-phosphate ester, stains simultaneously having blue stains are selected in the two culture mediums, the strains are subjected to streak cultivation in a BTB denitrification culture medium, and straining causing the culture medium to become blue are selected. The method has the advantages of being high in selectivity, small in workload and capable of greatly shortening screening cycle and the like.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to an efficient and rapid two-phase method for screening denitrifying phosphorus-accumulating bacteria. Background technique [0002] In my country's water pollution problems, water eutrophication is particularly serious, and nitrogen and phosphorus pollutants are the main factors leading to the eutrophication of many water bodies. Nearly 60 billion tons of wastewater carrying a large amount of nitrogen and phosphorus enter the water body every year, causing eutrophication of the water body, which in turn causes a large number of algal blooms in the water environment, consumes dissolved oxygen in the water, and affects the growth of aquatic organisms. Therefore, it is of great significance to develop efficient and economical technologies for nitrogen and phosphorus removal from polluted water. [0003] The bacterial ages of nitrifying bacteria and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/02C12N1/20
CPCC12N1/02C12N1/20
Inventor 王继勇江英杰陈加立肖挺
Owner WUHAN UNIV OF TECH
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