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Modified growth differentiation factor and preparation method and application thereof

A technology of growth and differentiation factors and differentiation factors, which is applied in the field of protein modification, can solve the problems of limited improvement of cognitive level, achieve the effects of reducing the content and activity of related enzymes, enhancing memory, and improving and treating Alzheimer's disease

Active Publication Date: 2017-11-07
江苏豪思生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although GDF11 is related to the cognitive level of the brain, its improvement on cognitive level is very limited, so it is necessary to find a new drug that can treat or effectively alleviate Alzheimer's disease with better effect

Method used

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  • Modified growth differentiation factor and preparation method and application thereof
  • Modified growth differentiation factor and preparation method and application thereof
  • Modified growth differentiation factor and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Example 1: The liquid containing His-tag-GDF11 (compound Ⅰ) prepared by coupling His-tag to the N-terminus of the active fragment of human growth differentiation factor 11

[0099] For the amino acid sequence of the His-tag-GDF11 prepared in this example, refer to the sequence table SEQ ID NO.8, wherein the His-tag has the amino acid sequence shown in SEQ ID NO.2, and the active fragment of human growth differentiation factor 11 has the SEQ ID NO . The amino acid sequence shown in 6, the C-terminal in the His-tag sequence, that is, the free carboxyl group on histidine, is coupled to the alpha-amino group at the N-terminal of the human growth differentiation factor to form a peptide bond.

[0100] The specific preparation method of compound I of this embodiment is as follows:

[0101] (1) Synthesize the gene encoding compound I by the method of whole gene synthesis: the gene has the base sequence shown in SEQ ID NO. figure 1 and SEQ ID NO.10): Nco I restriction site (ba...

Embodiment 2

[0106] Example 2: His-tag modification of the N-terminus of the active fragment of human growth differentiation factor 11 and purification with a nickel column

[0107] The His-tag in this example has the amino acid sequence shown in SEQ ID NO.2, the human growth and differentiation factor has the amino acid sequence shown in SEQ ID NO.6, and the His-tag is coupled to the N of the growth and differentiation factor 11 active fragment. end. Specifically, the His-tag modified growth and differentiation factor in this example is the His-tag-GDF11 obtained in Example 1.

[0108] The specific implementation methods of this embodiment are as follows:

[0109] (1) Equilibrate the nickel column: 2 milliliters of dextran beads are contained in the nickel column, and the column is equilibrated with 20 milliliters of 5mM Tris hydrochloric acid (pH8.0);

[0110] (2) Pass the supernatant containing the coupling product obtained in Example 1 through a nickel column at a flow rate of 2ml / mi...

Embodiment 3

[0113] Example 3: GST-tag is coupled with the active fragment of human growth differentiation factor 11 at the N-terminus to prepare a liquid containing GST-tag-GDF11 (compound Ⅱ)

[0114] The GST-tag in this example has the amino acid sequence shown in SEQ ID NO.3, the active fragment of human growth and differentiation factor 11 has the amino acid sequence shown in SEQ ID NO.6, and the carboxyl group at the C-terminal of the GST-tag is formed by a covalent bond. The peptide bond is coupled to the N-terminus of the active fragment of human growth differentiation factor 11, and the coupling product is GST-tag-GDF11. The amino acid sequence of the modified protein is shown in SEQ ID NO.9.

[0115] The specific implementation method of this embodiment is as follows:

[0116] (1) Synthesize the gene encoding GST-tag-GDF11 by the method of whole gene synthesis: the gene has the base sequence shown in SEQ ID NO.11 in the sequence listing, and the gene contains ( See details figu...

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Abstract

The invention discloses a modified growth differentiation factor, which is formed by coupling a modified article and a growth differentiation factor, wherein the growth differentiation factor is (a) a natural growth differentiation factor 11 from a mammal; (b) protein or polypeptide which is obtained through performing replacement and / or deletion and / or addition on the amino acid sequence of the natural growth differentiation factor 11 by one or a plurality of amino acid residues, has nerve cell regeneration promotion capability and is derived from the natural growth differentiation factor 11; or (c) protein or polypeptide which has at least 50 percent of homology with the amino acid sequence shown by (a) or (b), has the nerve cell regeneration promotion capability and is derived from the amino acid sequence shown by (a) or (b). The modified growth differentiation factor provided by the invention can be better used for preventing, relieving and treating Alzheimer's diseases, and also achieves the effect of enhancing the memory. In addition, a metabolite regulated and controlled by the growth differentiation factor has the application of diagnosing and screening the Alzheimer's diseases.

Description

technical field [0001] The invention relates to a modified protein, in particular to a modified growth differentiation factor and its preparation method and application. Background technique [0002] Alzheimer's syndrome, also known as senile dementia, is characterized by the gradual loss of memory and cognitive abilities of patients. Patients die within 3 to 9 years of diagnosis, accounting for 50% to 56% of clinical deaths. For the cause of the disease, the current understanding is that abnormally folded beta-amyloid and Tau proteins accumulate in the brain, which leads to the occurrence of Alzheimer's disease (Querfurth H.W.Alzheimer's Disease.N Engl J Med 2010; 362,329-344). [0003] The medicines currently on the market for the treatment of senile dementia are mainly western medicines, which are characterized in that they must be taken consistently for a long time, but the curative effect is small, and they can only alleviate some symptoms and cannot control the develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/51C07K19/00C12N15/12C12N15/62C12N15/70A61K38/18A61K47/60A61P25/28G01N33/68
CPCA61K47/60A61P25/28G01N33/6896A61K9/0002C12N15/62C12N15/70C07K14/51A61K38/00C07K2319/21C07K2319/23G01N2333/51G01N2800/50G01N2800/2821
Inventor 崔文宏
Owner 江苏豪思生物科技有限公司
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