Nithiazine degrading bacterium and application thereof
A technology for thiamethoxam and degrading bacteria, which is applied in the fields of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of long residual time and complex metabolic pathways, and achieves good removal effect and good degradation. Effect, low cost of production and use
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Embodiment 1
[0020] Example 1 Isolation and Identification of Bacterial Strains
[0021] Clothianidal-contaminated soil samples were made into a suspension, 10 mL was added to 100 mL of basal salt medium with a final concentration of clothianidin 300 mg / L, and enrichment culture was carried out at 30 °C and 160 r / min. The 10% inoculum was transferred to a new clothianidin inorganic salt medium, and after 4 consecutive transfers, the enrichment solution was obtained. Take 1.0 mL of the enrichment solution to make 10 -1 enriched solution, and then draw 1.0 ml prepared 10 -1 The enrichment solution was added to 9.0 ml sterile water, mixed thoroughly to make 10 -2 The enrichment solution, and so on, carry out gradient dilution on the enrichment solution. Draw 0.1 ml of the diluted solution of each gradient and spread it on the solid medium containing 300 mg / L of clothianidin inorganic salt (the formula is: 1.50 g of K per liter) 2 HPO 4 , 0.50 g KH 2 PO 4 , 0.20 g MgSO 4 ×7H 2 O, 1.00...
Embodiment 2
[0022] Shake flask degradation experiment of embodiment 2 Bacillus subtilis NZJ-37
[0023] In the medium containing 300 mg / L thianidoxal inorganic salts, the formula is: 1.50 g K2HPO4, 0.50 gKH2PO4, 0.20 g MgSO4×7H2O, 1.00 g NaCl, 1.00 g (NH4)2SO4 per liter, pH 7.0, NZJ-37 was inoculated at 1% inoculum amount, cultured with shaking at 30°C, and samples were taken every 6 hours for determination. Depend on figure 2 It can be seen that within 48 h the degradation of thianidoxal was more than 90%.
Embodiment 3
[0024] Example 3 Preparation of bacterium agent containing Bacillus subtilis NZJ-37
[0025] The original species of Bacillus subtilis NZJ-37 screened and isolated in Example 1 was activated on a petri dish, and its degradation performance was measured, and it was inoculated on the inclined surface of a test tube for later use. The test tube was inoculated in a 1000ml shake flask containing 200ml LB medium. The formula of LB medium was: yeast extract 5.00g / L, peptone 10.00g / L, NaCl 10.00g / L, pH 7.0, constant temperature shaking culture to logarithmic phase , ready to inoculate the seed pot. The seed tank is 500 liters, the feeding volume is 400 liters, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask strains into a 500-li...
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