A kind of tomato immature embryo culture method and culture medium thereof

Abstract

The invention belongs to the technical field of immature embryo culture, and discloses a tomato immature embryo culture method and a medium thereof. The method comprises the following steps: disinfection operation: tomato young fruits are picked, tap water is firstly used for fully flushing, surface disinfection is carried out with 10% sodium hypochlorite for 15 minutes, flushing with sterile water is carried out for 3-5 times, and disinfection operation is completed; aseptic inoculation: immature embryos are taken out and aseptic inoculation is carried out under an aseptic condition, 10 immature embryos are inoculated in each bottle, rooting and germination cases are momentarily observed; statistics and comparison of results; culture of immature embryos: after inoculation, culture temperature is 25+ / -1 DEG C, illumination is carried out for 12 hours every day, and artificial culture is carried out; spinning of bottles for seedlings: tomato seedlings grow, bottles are span, and seedling number in each bottle is five; hardening of seedlings: bottlenecks are opened before transplanting, hardening of seedlings is carried out in shade for 1-3 days; transplanting of seedlings: when the height of the seedlings is 5-7cm, the seedlings are taken out and transplanted to nutrition bowls with nutrient soil, enough watering is carried out, and high humidity is kept; after 5-7 days, plants are regenerated, and normal growth and development can be carried out.

Description

technical field [0001] The invention belongs to the technical field of immature embryo culture, and in particular relates to a method for cultivating tomato immature embryos and a culture medium thereof. Background technique [0002] At present, tomato is rich in nutrition and has a large cultivation area. It occupies a very important position in my country's agricultural production and crop breeding. However, the conventional seed collection operation of tomato is relatively complicated, and it is necessary to take out the seeds after the tomato fruit is fully ripe, and then sow them again after sufficient post-ripening procedures. As a result, the traditional breeding technology has limitations such as long breeding cycle and limited yield increase potential. Therefore, it is very necessary and urgent to explore the technical means of improving breeding efficiency with short breeding cycle for the genetics of important crops such as tomato. [0003] To sum up, the problem...

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Problems solved by technology

[0003] In summary, the existing problems in the prior art are: the routine tomato seed collection operation is relatively complicated and takes a long time, and the tomato growth cycle reaches about 120 days, resulting in extremely low breeding efficiency

At present, southern propagation or greenhouse facilities are generally used to improve breeding efficiency, but the characteristics of tomato seeds themselves determine that they must be matured after picking to germinate normally. Therefore, existing technologies and methods cannot fundamentally shorten the tomato growth cycle and improve breeding efficiency.

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