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Placenta extract and its preparation method and application

A technology of extract and placenta, which is applied in the field of placenta extract and its preparation, can solve problems such as difficulty in maintaining the three-dimensional structure and activity of polypeptides, changes in the structure of polypeptide components, and loss of physiological activity, so as to achieve no preparation cycle and promote ovarian function Reconstruction, good effect of premature ovarian failure

Active Publication Date: 2020-08-18
GUANGZHOU ZHUNYOU BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction efficiency of the solvent method is low, and unnecessary substances are mixed in the dissolved components; the hydrochloric acid hydrolysis method will cut off the amino acid chain arbitrarily, and it is difficult to maintain the three-dimensional structure and activity of the polypeptide in the placenta extract; Enzymes can only catalyze one chemical reaction each, with strong specificity, and because enzymes can mechanically and irreversibly cut off specific amino acid combinations, the structure of polypeptide components in the placenta extract will be changed and the physiological activity will be lost; therefore, it is necessary to treat the placenta The extraction method was improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 A kind of preparation method of placenta extract

[0032] The preparation method of the placenta extract of the present embodiment comprises the following steps:

[0033] (1), select 100 mL of fresh healthy placenta tissue after selection;

[0034] (2) Fully wash the placental tissue and cut it into small pieces, remove the blood vessels and mucosal tissue, put it in 100mL50mmoL / L acetic acid-sodium acetate buffer solution (pH4.0) for homogenization; place the homogenate at 4°C Incubate in a water bath for 2.5 hours, stirring occasionally during the period; after returning to room temperature, refrigerate and centrifuge (4°C, 6600r / min, 30min) to obtain a precipitate and about 150mL of supernatant;

[0035] (3), at 4°C, slowly add ethanol (-20°C pre-cooling) 6 times the volume of the supernatant to the supernatant in step (2), stir continuously during the dropping process, refrigerate and centrifuge (4°C, 6600r / min, 30min) after taking the supernatant;

...

Embodiment 2

[0041] Embodiment 2 A kind of preparation method of placenta extract

[0042] The preparation method of the placenta extract of the present embodiment comprises the following steps:

[0043] (1), select 500mL of fresh healthy placenta tissue after selection;

[0044] (2) Fully wash the placental tissue and cut it into small pieces, remove the blood vessels and mucosal tissue, put it in 500mL30mmoL / L acetic acid-sodium acetate buffer solution (pH5.0) for homogenization; place the homogenate at 4°C Incubate in a water bath for 3 hours, stirring from time to time; after returning to room temperature, refrigerate and centrifuge (4°C, 6600r / min, 30min) to obtain a precipitate and about 750mL supernatant;

[0045] (3), at 4°C, slowly add ethanol (pre-cooled at -20°C) 8 times the volume of the supernatant to the supernatant in step (2), stir continuously during the dropping process, refrigerate and centrifuge (4°C, 6600r / min, 30min) after taking the supernatant;

[0046] (4) Evapo...

Embodiment 3

[0051] Embodiment 3 A kind of preparation method of placenta extract

[0052] The preparation method of the placenta extract of the present embodiment comprises the following steps:

[0053] (1), select 1000mL of fresh healthy placenta tissue after selection;

[0054] (2) Fully wash the placental tissue and cut it into small pieces, remove the blood vessels and mucosal tissue, put it in 1000mL70mmoL / L acetic acid-sodium acetate buffer (pH6.0) for homogenization after chopping; place the homogenate at 4°C Incubate in a water bath for 2 hours, stirring from time to time; after returning to room temperature, refrigerate and centrifuge (4°C, 6600r / min, 30min) to obtain a precipitate and about 1500mL supernatant;

[0055] (3), at 4°C, slowly add ethanol 10 times the volume of the supernatant (pre-cooled at -20°C) to the supernatant in step (2), stir continuously during the dropping process, refrigerate and centrifuge (4°C, 6600r / min, 30min) after taking the supernatant;

[0056]...

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Abstract

A placental extract and a preparation method therefor. The preparation method comprises: freezing placental tissue, extracting effective ingredients, and purifying said ingredients to obtain the final product. The placental extract may be used to treat premature ovarian failure.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to a placenta extract and its preparation method and application. Background technique [0002] Premature ovarian failure (POF) refers to a disease caused by various etiologies in which women under the age of 40 experience amenorrhea, decreased estrogen secretion, and increased gonadotropin levels due to ovarian failure. [0003] The incidence of POF is increasing year by year, and the proportion of POF in female infertility patients is also increasing year by year. The two major threats of POF to women's reproductive health are the decrease of estrogen level and the loss of fertility. The decrease of estrogen level increases the risk of osteoporosis and coronary heart disease in women. Women who are in the childbearing period have early amenorrhea and loss of fertility, which will lead to a series of psychological and social problems, such as prone to de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/50A61P15/08
CPCA61K35/50
Inventor 吴道贫李文禄赖小华陶然
Owner GUANGZHOU ZHUNYOU BIOLOGICAL TECH CO LTD
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