Method for biomimetic synthesis of hydroxyapatite by imidazole-4,5-dicarboxylic acid-modified chitosan

A technology for synthesizing hydroxyapatite and dicarboxylic acid, applied in chemical instruments and methods, phosphorus compounds, tissue regeneration, etc., can solve the problems of HAP not having biological activity, pH responsiveness, application limitations, etc., and achieve good pH response Sexuality, good biological activity, and mild reaction conditions

Inactive Publication Date: 2017-11-24
PUTIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of good biological activity and pH responsiveness of HAP, its application is limited.

Method used

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  • Method for biomimetic synthesis of hydroxyapatite by imidazole-4,5-dicarboxylic acid-modified chitosan
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  • Method for biomimetic synthesis of hydroxyapatite by imidazole-4,5-dicarboxylic acid-modified chitosan

Examples

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Embodiment 1

[0032] Synthesis of imidazole-4,5-dicarboxylic acid modified chitosan (HIDCCS)

[0033] Weigh 1.2000g of chitosan and dissolve in 100mL (100mL0.10mol L -1 )2-( N -morpholine) ethanesulfonic acid buffer solution (pH=5.5), using a three-necked flask as a reaction vessel, add 1.9978g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC HCl) as condensation agent, 0.8567g N -Hydroxysuccinimide was used as a coupling agent, and after electric stirring, 3.4860 g of imidazole-4,5-dicarboxylic acid was added. The mixture was placed at room temperature with electric magnetic stirring for 24 h to make it fully stirred evenly. Put the stirred mixture mucus into the treated dialysis bag and tie it to prevent leakage. Dialyze in distilled water for a week (change distilled water about 4-6 times a day). The dialysate was transferred to a Petri dish, wrapped with plastic wrap and placed in a -18°C freezer for 24 hours. Then take it out, poke several small holes on the plas...

Embodiment 2

[0035] The method for biomimetic synthesis of spherical hydroxyapatite by using imidazole-4,5-dicarboxylic acid modified chitosan comprises the following steps:

[0036] Take an appropriate amount of calcium chloride and disodium hydrogen phosphate and dissolve it in the simulated body fluid. The system in the experiment is fed with a Ca / P substance ratio of 1.67. Use a beaker to take 25 mL of sodium dihydrogen phosphate solution (0.12mol L -1 ), using a collector magnetic stirrer to control the temperature 37 oc. Then add imidazole-4,5-dicarboxylic acid modified chitosan 0.006g, then take 25mL calcium chloride (0.2mol L -1 ), added dropwise at a rate of 1 drop per second, during the process of adding calcium chloride, use ammonia water to control the pH of the solution between 10.00 and 10.50. After the dropwise addition, stir for 30min, age for 48h, carry out suction filtration, wash repeatedly with absolute ethanol and distilled water, and control the temperature with an ...

Embodiment 3

[0038] Biomimetic synthesis of rod-shaped hydroxyapatite using imidazole-4,5-dicarboxylic acid modified chitosan material at different temperatures (32, 37, 42, 47 degrees)

[0039] Take an appropriate amount of calcium chloride and disodium hydrogen phosphate and dissolve it in the simulated body fluid. The system in the experiment is fed with a Ca / P substance ratio of 1.67. Use a beaker to take 25 mL of sodium dihydrogen phosphate solution (0.12mol L -1 ), using a collector magnetic stirrer to control the temperature 32 o c. Then add imidazole-4,5-dicarboxylic acid modified chitosan 0.006g, then take 25mL calcium chloride (0.2mol L -1 ), added dropwise at a rate of 1 drop per second, during the process of adding calcium chloride, use ammonia water to control the pH of the solution between 10.00 and 10.50. After the dropwise addition, stir for 30min, age for 24h, carry out suction filtration, wash repeatedly with absolute ethanol and distilled water, and control the tempera...

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Abstract

The invention belongs to the biological bone tissue engineering, and concretely relates to a method for biomimetic synthesis of hydroxyapatite by imidazole-4,5-dicarboxylic acid-modified chitosan. Through biomimetic synthesis, a biomineralization mechanism is introduced into a HAP synthesis process, the imidazole-4,5-dicarboxylic acid-modified chitosan having pH responsiveness is taken as an organic matrix, so that the hydroxyapatite having a specific structure and modified surface is obtained. The hydroxyapatite through biomimetic synthesis is hopeful for increasing the biological activity and mechanical property, and provides a reference for biomimetic synthesis of other biominerals.

Description

technical field [0001] The invention belongs to the field of biological bone tissue engineering, and in particular relates to a method for bionically synthesizing hydroxyapatite by imidazole-4,5-dicarboxylic acid modified chitosan. Background technique [0002] In the mid-1970s, Roy first synthesized hydroxyapatite (Hydroxyapatite, HAP), creating a new era of artificial bone materials. Hydroxyapatite is a kind of calcium phosphate. Its chemical composition, crystal structure, physical and chemical properties are similar to the inorganic components of normal human bone. The results of animal experiments show that hydroxyapatite can replace Ca and P, and the hydroxyl group is bonded to human tissue through hydrogen bonds to achieve perfect affinity, excellent biocompatibility, no immune rejection, and has a certain bone conductivity, providing excellent physiological conditions for the deposition and regeneration of new bone scaffold, and can form a direct osseointegration wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/12A61L27/50C01B25/32
CPCA61L27/12A61L27/20A61L27/50A61L2430/02C01B25/325C01P2002/72C01P2002/82C01P2004/03C08L5/08
Inventor 陈彰旭叶晨光林永龙吴宝蓉谢慧敏林素津
Owner PUTIAN UNIV
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