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Peanut Glutamyl t-RNA Reductase and Its Application

A technology of glutamyl and reductase, applied in the field of genetic engineering

Inactive Publication Date: 2020-06-09
BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the peanut in the above prior art HEMA The problem of blank research on the regulation of genes in the physiological process of plant stress and aging, the application discloses a peanut glutamyl t-RNA reductase

Method used

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  • Peanut Glutamyl t-RNA Reductase and Its Application
  • Peanut Glutamyl t-RNA Reductase and Its Application
  • Peanut Glutamyl t-RNA Reductase and Its Application

Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1. AhhemA gene segregation

[0026] RNA was extracted from peanut leaves using an RNA kit (purchased from Tiangen Biochemical Technology Co., Ltd.) and reverse transcribed. According to the gene sequence, design primers, the primer sequence is: 5'-ATGGCTGTTTCGACGAGC-3' and 5'-TTAGCTGTCACTATGGTT-3' for polymerase chain reaction (PCR), the PCR product is detected by agarose gel electrophoresis and estimated The fragment size conforms to (1626 bp) ( figure 1 ).

[0027] The obtained PCR product was connected with pMD18-T cloning vector, and transformed into Escherichia coli. The positive clones were quickly screened by colony PCR, the plasmids of the positive clones were extracted, and the plasmids were cut using the enzyme cutting sites inside the vector to obtain bands with the same size as the PCR products. Send the identified bacterial solution to Shanghai Bioengineering Company for sequencing, see sequence 3 in the sequence list, use Blast, DNAman or DNAclub sof...

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Abstract

The invention relates to the field of gene engineering technology, in particular to a peanut glutamoyl t--RNA reductase, and further to an application of the peanut glutamoyl t--RNA reductase in regulating the expressions of chlorophyll and 5 calmodulin. The invention has the following beneficial effects: the invention has identified functions of the peanut glutamoyl t--RNA reductase, namely, regulating the expressions of chlorophyll and calmodulin, and clarified the interaction and relationship between the reductase and calcium signals, thereby laying the theoretical foundation for later researches.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a peanut glutamyl t-RNA reductase, and also relates to the application of the peanut glutamyl t-RNA reductase. Background technique [0002] Chlorophyll (Chlorophyll, Chl) is a fat-soluble pigment that exists in chloroplasts in higher plants. When plants perform photosynthesis, chlorophyll plays an extremely important role. It can absorb and convert solar energy, and in this process, decompose water molecules to produce oxygen molecules and reduced oxygen, which plays a very important role in the cell electron transport chain. Therefore, chlorophyll plays a very important role in the growth and development of plants and the formation of crop quality and yield. The starting material for chlorophyll biosynthesis is L-glutamyl-tRNA. After the catalysis of GluTR, L-glutamyl-t RNA is reduced to L-glutamate-1-semialdehyde; then glutamic acid Ester-1-semialdehyde transamin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H5/00C12N9/02C12N15/53A01H6/54
CPCC12N9/0008C12N15/8269C12N15/8271C12Y102/0107
Inventor 杨莎赵璐颖李新国万书波张佳蕾郭峰耿耘孟静静
Owner BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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