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Kit for detecting clinical depressive emotion and depression and application of kit

A kit and depression technology, applied in the biological field, can solve the problems of depression and depression-specific examination items, misdiagnosis, missed diagnosis, etc., achieve good clinical depression and depression detection ability, and the detection application method is simple and simplified effect of the program

Inactive Publication Date: 2017-11-24
THE AFFILIATED HOSPITAL OF SOUTHWEST MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the pathogenesis of depression is still unclear, and its diagnostic methods do not have specific examination items for depression and depressed mood. The diagnosis and curative effect are mainly based on the patient's symptom description, mental state examination and clinical behavioral observation. , lack of reliable biological indicators with clinical diagnostic significance, resulting in high missed diagnosis and misdiagnosis
Since the persistent psychological disorder appears for at least two weeks, and the severe state can only be confirmed for at least two years, the current diagnosis lacks rapid and sensitive indicators, and it is difficult to confirm whether the patient has the disease or has developed it in a short period of time, let alone measures. Early prevention to avoid tragedy

Method used

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  • Kit for detecting clinical depressive emotion and depression and application of kit
  • Kit for detecting clinical depressive emotion and depression and application of kit
  • Kit for detecting clinical depressive emotion and depression and application of kit

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Experimental program
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Effect test

Embodiment 1

[0069] According to the PHQ score, 72 patients with no depression, mild depression, moderate and above depression, and depression were screened. After the blood donors agreed, venous blood was drawn from four groups of blood donors: 22 venous blood of the non-depression group , 20 parts of venous blood of mild depression group, 22 parts of moderate and above group venous blood, 8 parts of venous blood of depression group, room temperature coagulation, centrifugation gets serum, adopts the kit provided by the present invention to detect, and its detection result is as shown in the table 3 shows:

[0070]

[0071] table 3

[0072] According to the aforementioned standard curve equation, the Ct value of each sample is known to be the absolute content (copies) of serum miRNA-221-3p. As shown in Table 2: we analyzed the average concentration and range of serum miRNA-221-3p of patients with different PHQ scores and found that: mild (PHQ average score 6.03) and moderate and above...

Embodiment 2

[0074] According to the PHQ score, screen 60 patients with mild depression, moderate and above depression in the perioperative period, and after the consent of the blood donor, take the patient's venous blood, coagulate at room temperature, and centrifuge to get serum, using the kit provided by the invention Detect, its detection result is abscissa with serum miRNA-221-3p concentration, PHQ-9 score is ordinate, draws the result as follows Figure 5 As shown, the linear regression equation is: Y=2E-07X+5.529, R 2 =0.2636, correlation coefficient R=0.513. According to the data distribution, two straight lines are added for further analysis, and the serum miR-221-3p concentration at the intersection of the straight lines is 1.7*10 7 copies / μg RNA, corresponding to a PHQ-9 score of 6 points; patients located on the left side of the intersection point, that is, the serum miR-221-3p concentration is less than 1.7*10 7 Copies / μg RNA, although the PHQ-9 depression score is high, the...

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Abstract

The invention provides a serum microRNA, miR-221-3p applicable to a detection reagent or a detection kit for detecting depressive emotion or depression, a standard product of miR-221-3p obtained by virtue of in-vitro transcription of genetic engineering and a standard curve corresponding to a Ct value for determining an absolute content of miR-221-3p in the serum and miRNA-221-3p fluorescent quantitative PCR in the serum of a depressive emotion or depression patient. The procedures for detecting the depression are simplified. The invention provides a detection kit for clinical depressive emotion and depression based on serum microRNA; and by adding the miR-221-3p standard product, the detection is simple, the sensitivity is high, the specificity is high, the result is accurate, and the capacity for detecting the clinical depressive emotion and the depression is good.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for detecting clinical depression and depression and its application. Background technique [0002] Major depression is an affective disorder characterized by low mood, anhedonia and loss of interest and other mental and psychological syndromes, accompanied by other psychological and physical symptoms. The clinical manifestations of depression vary greatly, and its main clinical manifestations can be divided into two categories: core symptoms and accompanying symptoms (psychological symptoms and somatic symptoms). Low mood or mood, loss of interest, and loss of enjoyment are key symptoms of depression. Among them, depression refers to the patient's experience of low mood and sadness; anhedonia refers to the patient's lack of enjoyment in various previously favorite activities; loss of fun refers to the patient's inability to experience pleasure from life, or Anhedonia. The ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6851C12Q1/6883C12Q2600/158C12Q2600/178C12Q2531/113C12Q2563/107C12Q2545/114
Inventor 王晓斌冯建国李茂刘力
Owner THE AFFILIATED HOSPITAL OF SOUTHWEST MEDICAL UNIV