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Fermented milk and preparation method thereof

A technology for fermented milk and skim milk, which is applied in the field of fermented milk, can solve problems such as inapplicability to the treatment of anterior teeth, and achieves the effects of significant inhibitory activity and good stability

Pending Publication Date: 2017-12-01
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few drugs under control at present, and silver ammonium nitrate is one of the few. Silver ammonium nitrate is a kind of antiseptic and bactericidal drug, which has antiseptic, astringent, bactericidal and corrosive effects, but the drug can stain teeth, so it is not recommended Suitable for anterior teeth treatment

Method used

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  • Fermented milk and preparation method thereof
  • Fermented milk and preparation method thereof
  • Fermented milk and preparation method thereof

Examples

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preparation example Construction

[0019] In a specific embodiment, a method for preparing fermented milk is provided, the method comprising the following steps: inoculating Streptococcus salivarius CGMCC NO.13308 in sucrose-containing skim milk for fermentation to obtain fermented milk.

[0020] In the preparation method of the above technical solution, for the first time, a strain of Streptococcus salivarius CGMCC NO.13308, which has the effect of inhibiting the growth of Streptococcus mutans and its biofilm formation, is used for fermentation with sucrose-containing skimmed milk as a medium to prepare an anti-caries Fermented milk discloses that Streptococcus salivarius CGMCC No. 13308 has a new application of making fermented skim milk generate anti-caries substances. Compared with other conventionally fermented skim milk strains, the fermented milk prepared by Streptococcus salivarius CGMCC NO.13308 has more significant inhibitory activity on the biogenesis of Streptococcus mutans, and has better stability....

Embodiment 1

[0032] 1. Materials and methods

[0033] (1) Preparation of seeds (fermentation strains):

[0034]Streptococcus salivarius CGMCC No.13308: Dissolve the freeze-dried powder of Streptococcus salivarius CGMCC No.13308 with a small amount of sterile distilled water, and get a ring with an inoculation loop and streak it on M17 solid medium (purchased from OXOID Co., UK), After anaerobic culture at 37°C for 24 hours, single colonies were picked with an inoculation loop and put into 10 mL of M17 liquid medium (purchased from OXOID Co., UK). Take out after 24 hours of oxygen culture, inoculate in M17 liquid medium (purchased from OXOID Co., UK) with 2% (v / v) inoculum, after 24 hours of anaerobic culture at 37 ° C, the culture is centrifuged at 15000 rpm for 10 minutes, and the supernatant is discarded. After washing the bacterium twice with sterile distilled water, suspend it with the original culture volume of sterile distilled water to obtain seeds for fermentation. The bacterial c...

Embodiment 2

[0044] 1. Materials and methods

[0045] (1) Preparation of seeds (fermentation strain): same as Example 1.

[0046] (2) Preparation of skim milk: mix 12% skim milk powder with distilled water, fully dissolve, then add 5% (w / v) sucrose to fully dissolve, sterilize at 95°C for 20min, and cool to At room temperature, skim milk is obtained.

[0047] (3) The detection method of the activity of Streptococcus salivarius inhibiting Streptococcus mutans and the detection method of the inhibition rate of fermented milk to the biofilm formation of Streptococcus mutans: the same as in Example 1.

[0048] 2. Preparation of fermented milk

[0049] Streptococcus salivarius CGMCC NO.13308 seeds were aseptically inoculated with 4% (v / v) inoculum in 12% (w / w) skim milk containing 5% (w / v) sucrose, and cultured anaerobically at 42°C for 3 hours. fermented milk B.

[0050] 3. Determination of inhibitory activity

[0051] The fermented milk B was respectively tested for its inhibitory activi...

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Abstract

The invention provides fermented milk and a preparation method thereof. The preparation method comprises the step of inoculating streptococcus salivarius CGMCC NO.13308 into cane sugar-containing skimmed milk for fermenting to obtain the fermented milk. According to the method, streptococcus salivarius CGMCC NO.13308 is adopted to ferment skimmed milk for the first time, and has an inhibiting activity for streptococcus mutans growth and biological membrane formation thereof, which discloses that the streptococcus salivarius CGMCC NO.13308 has novel application of fermented skimmed milk for generating decayed tooth resistant substances. Compared with other conventional fermented skimmed milk, the fermented milk prepared from the streptococcus salivarius CGMCC NO.13308 has remarkable inhibiting activity for streptococcus mutans microbe formation, and has good stability.

Description

technical field [0001] The invention belongs to the technical field of fermented milk, and in particular relates to fermented milk and a preparation method thereof. Background technique [0002] Dental caries, commonly known as caries, tooth decay, is a bacterial disease, can be secondary to pulpitis and periodontitis, and even cause alveolar bone and jaw inflammation. If it is not treated in time, the lesion will continue to develop, forming cavities, and eventually the crown will be completely destroyed and disappeared. The final result of its development is tooth loss. Dental caries is characterized by high incidence and wide distribution. The disease is the main common disease of the oral cavity and one of the most common human diseases. The World Health Organization has listed it as one of the three major diseases of human beings, along with tumors and cardiovascular diseases. [0003] There are many factors that cause dental caries, such as bacteria, oral environment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/123
CPCA23C9/1236A23V2400/245
Inventor 韩瑨刘振民于亚男冯华峰
Owner BRIGHT DAIRY & FOOD CO LTD
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