Method for establishing acute hyperuricemia rat model
A technology of hyperuricemia and rat model, which is applied in the direction of pharmaceutical formulations, organic active ingredients, preparations for in vivo tests, etc., can solve the problems of low molding rate and increased mortality, and achieve good stability and reproduction Powerful and simple construction method
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Embodiment 1
[0044] Example 1: Effectiveness and dose-effect relationship experiment of inosine-induced acute hyperuricemia in rats
[0045] Select 56 Wistar rats, half male and half female, body weight 240-260g, randomly divided into 7 groups, 8 rats in each group, inject inosine according to the following doses, the doses are: 50mg / kg, 100mg / kg, 150mg / kg, 200mg / kg, 300mg / kg, 400mg / kg, the control group was equal volume of normal saline. After 0h, 0.5h, 1h, 2h, 4h, 8h, and 24h of the drug, 0.5ml of blood was collected from the tail vein, and the serum uric acid value was determined by the phosphotungstic acid method.
[0046] The results showed that compared with the control group, the uric acid value of the 50mg / kg, 100mg / kg, and 200mg / kg dose groups of rats increased, but the increase failed to reach the crystal concentration of uric acid in the blood of 416.5μmol / L. The serum uric acid values of the 300mg / kg dosage group and the 400mg / kg dosage group increased at 0.5h and 1h respect...
Embodiment 2
[0051] Example 2: Application of Acute Hyperuricemia Rat Model in the Screening of Drugs for Lowering Uric Acid——Experiment on the Effect of Allopurinol on Blood Uric Acid in Rats with Hyperuricemia
[0052] Allopurinol is an inhibitor of xanthine oxidase and is currently a commonly used urate-lowering drug. Current data show that allopurinol can reduce the uric acid level of rats with hyperuricemia induced by potassium oxonate, and the effective dose is 22mg / kg. In this experiment, 32 normal Wistar rats, half male and half female, weighing 240-260g, were randomly divided into 4 groups with 8 rats in each group. The first group was injected with 300mg / kg of inosine intraperitoneally; Inosine, followed by allopurinol at a dose of 22 mg / kg; group 3 was injected with inosine at a dose of 300 mg / kg, followed by allopurinol at a dose of 30 mg / kg; the control group was injected with an equal volume of physiological brine. After 0h, 0.5h, 1h, 2h, 4h, and 8h of the drug, 0.5ml of bl...
Embodiment 3
[0057] Example 3: Preliminary Toxicity and Safety Study of Inosine on Rats
[0058] In this embodiment, rats were intraperitoneally injected with inosine at different doses of 50mg / kg, 100mg / kg, 150mg / kg, 200mg / kg, 300mg / kg, 400mg / kg, 800mg / kg, 1000mg / kg, and 1200 mg / kg respectively. 72 rats, half male and half female, weighing 240-260 g, were set up as a control group, with 6 Wistar rats in each group, were observed continuously for 15 days, and the animal poisoning and death conditions were recorded. Two animals in the 1000mg / kg dose group died on the spot after taking the medicine respectively. Animals in the other groups did not die, did not have any discomfort, moved freely, took water and food, defecated normally, and were generally in good condition. All animals were sacrificed 15 days after the intraperitoneal injection, and grossly dissected, and the liver, kidney and related organs were observed with naked eyes, and no abnormalities were found in the results.
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