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Microfluidic chip-based single-cell sampling and in situ detection mass spectrometer interface device

A microfluidic chip and detection device technology, applied in the field of cell research, can solve analysis and other problems, achieve the effects of improved sensitivity, fast extraction speed, and improved ionization efficiency

Active Publication Date: 2021-01-05
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it cannot analyze cell membrane, cell surface protein, nucleus, etc.

Method used

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  • Microfluidic chip-based single-cell sampling and in situ detection mass spectrometer interface device
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  • Microfluidic chip-based single-cell sampling and in situ detection mass spectrometer interface device

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Embodiment Construction

[0019]In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "vertical", "horizontal", "top", "bottom", "inner", " The orientation or positional relationship indicated by “outside” is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation to The specific azimuth structure and operation cannot be understood as a limitation of the present invention. In addition, the terms "first", "second", etc. are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance. The present invention will be described in detail below with reference to the drawings and embodiments.

[0020]Such asfigure 1 As shown, the present invention provides a microfluidic chip-based single c...

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Abstract

The invention relates to a single cell sampling and in-situ detection mass spectrometry interface device based on a microfluidic chip. The device is characterized by comprising the microfluidic chip, a single cell liquid ink-jet printing device and a detection device, wherein cell samples are cultured in a petri dish, and the microfluidic chip arranged on a fixing rack is located above the petri dish; the cell samples enter the single cell liquid ink-jet printing device through the microfluidic chip, single cell liquid droplets are formed by the single cell liquid ink-jet printing device and fall into the detection device, and all-component information of each single cell is obtained. The device integrates the microfluidic chip, formation of the single cell liquid droplets, single cell liquid ionization and detection and can perform acquisition, solvent replacement, liquid droplet formation and online mass spectrometric detection on any single cell.

Description

Technical field[0001]The invention relates to the technical field of cell research, in particular to an interface device for single cell extraction and in-situ mass spectrometry detection based on a microfluidic chip.Background technique[0002]As the structural and functional units that make up most organisms, cell research has always attracted much attention. These studies are of great significance to human drug metabolism, biological behavior, and signal transmission. Traditional cell research methods are based on a large number of cells. Whether it is cell morphology or metabolomics research, it is the average level of a large number of cells. These research methods cannot be used to study cell differences. In recent years, scientists have paid more and more attention to single-cell research, and the role of single-cell research has become more and more important. It not only involves drug metabolism and new drug development, but also explains cell molecular mechanisms and cell pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/34
CPCC12M23/16C12M33/00C12M41/36C12M41/48
Inventor 林金明毛思锋
Owner TSINGHUA UNIV