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Method for screening arbuscular mycorrhizal fungi spores from soil

A technology of arbuscular mycorrhizal fungi and soil, applied in the field of microorganisms, can solve the problems of large human error, high spore loss rate, and low screening efficiency, and achieve the effect of simple operation, high loss rate, and little influence of human factors

Inactive Publication Date: 2017-12-08
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the wet sieving and dumping method is usually used to screen the arbuscular fungal spores from the soil, but this method has the disadvantages of large human error, low screening efficiency, the individual density of the screened arbuscular fungal spores is generally too large or too small, and the spores are lost. The disadvantage of high rate is not conducive to extensive and in-depth research on arbuscular mycorrhizal fungi

Method used

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  • Method for screening arbuscular mycorrhizal fungi spores from soil
  • Method for screening arbuscular mycorrhizal fungi spores from soil
  • Method for screening arbuscular mycorrhizal fungi spores from soil

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Embodiment 1

[0029] A method for screening arbuscular mycorrhizal fungal spores from soil, comprising the following steps:

[0030] (1) Source soil collection: collect the whole plant of Artemisia annua and its root soil from a certain place in Chongzuo, and store it at 25°C for future use;

[0031] (2) Propagation and cultivation: the above-mentioned collected root soil and sterilized sand are mixed evenly in a volume ratio of 1:1 to obtain a mixed soil, and the mixed soil is put into a flower pot in an amount of 3Kg / pot; Plant germinated corn seeds with consistent growth in the above flower pots, and 4 grains / pot; after the germinated seeds of corn emerge, keep 2 corn seedlings with consistent growth in each flower pot, and cultivate them in the greenhouse for 95 days;

[0032] Wherein, the sterilized sand is river sand after being sterilized at 120°C for 4 hours;

[0033] The germinated corn seeds are cultivated by the following method: soak the selected corn seeds in hydrogen peroxide...

Embodiment 2

[0043] A method for screening arbuscular mycorrhizal fungal spores from soil, comprising the following steps:

[0044] (1) Source soil collection: collect the whole plant of Artemisia annua and its root soil from a certain place in Chongzuo, and store it at 25°C for future use;

[0045] (2) Propagation and cultivation: the above-mentioned collected root soil and sterilized sand are mixed evenly in a volume ratio of 1:1 to obtain a mixed soil, and the mixed soil is put into a flower pot in an amount of 3Kg / pot; Plant germinated corn seeds with consistent growth in the above flower pots, and 4 grains / pot; after the germinated seeds of corn emerge, keep 2 corn seedlings with consistent growth in each flower pot, and cultivate them in the greenhouse for 95-100 days;

[0046] Wherein, the sterilized sand is river sand after being sterilized at 122°C for 4 hours;

[0047] The germinated corn seeds are cultivated by the following method: soak the selected corn seeds in hydrogen pero...

Embodiment 3

[0057] A method for screening arbuscular mycorrhizal fungal spores from soil, comprising the following steps:

[0058] (1) Source soil collection: collect the whole plant of Artemisia annua and its root soil from a certain place in Chongzuo, and store it at 25°C for future use;

[0059] (2) Propagation and cultivation: the above-mentioned collected root soil and sterilized sand are mixed evenly in a volume ratio of 1:1 to obtain a mixed soil, and the mixed soil is put into a flower pot in an amount of 3Kg / pot; Plant germinated corn seeds with consistent growth in the above flower pots, and 4 grains / pot; after the germinated seeds of corn emerge, keep 2 corn seedlings with consistent growth in each flower pot, and cultivate them in the greenhouse for 100 days;

[0060] Wherein, the sterilized sand is river sand after being sterilized at 125°C for 4 hours;

[0061] The germinated corn seeds are cultivated by the following method: soak the selected corn seeds in hydrogen peroxid...

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Abstract

The invention belongs to the field of microbial technology and specifically discloses a method for screening arbuscular mycorrhizal fungi spores from soil. The screening method disclosed by the invention comprises the following steps: (1) source soil collection: collecting artemisia annua root soil; (2) propagation culture: mixing the root soil in (1) with sterilized sand, and cultivating germinated corn seeds; (3) collection of a soil sample: collecting the soil around the root system of a corn seedling; (4) collection of screened sample: dissolving the soil sample and screening with a three-layer filter screen; (5) centrifugation: performing twice centrifugation; (6) collection and counting of spores; (7) flaking. The screening method disclosed by the invention has the characteristics of low personal error, convenience and easiness, high screening efficiency and low loss rate of spores; moreover, the individual density of the screened arbuscular mycorrhizal fungi spores is uniform.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms, in particular to a method for screening arbuscular mycorrhizal fungus spores from soil. 【Background technique】 [0002] Mycorrhiza is a mutualistic symbiosis established by fungi and plant roots, among which Arbuscular Mycorrhizal Fungi (AMF) is the most widely distributed in nature. Whether monocot or dicot, most of them can form AMF symbiosis. Arbuscular mycorrhizal fungi can not only promote the absorption and utilization of mineral nutrients and water by crops, especially the absorption of phosphorus, thereby improving the yield and quality of plants, but also improve soil structure as a multi-functional high-efficiency bio-fertilizer, reducing the use of chemical fertilizers and pesticides. It is of far-reaching significance for the protection of the environment, landscaping and sustainable development of agriculture. The spores of arbuscular mycorrhizal fungi are important reprod...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/2813G01N1/30
Inventor 黄京华韦竹立马俊卿
Owner GUANGXI UNIV
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