A kind of compound extract of kelp and sea cucumber and its preparation method and application
A compound extract and technology of sea cucumber, which is applied in the field of food processing, can solve the problems of many active ingredients and general weight loss effect
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Embodiment 1
[0047] 1. Raw material processing of sea cucumber and kelp
[0048] Wash fresh sea cucumbers with clean water, freeze-dry them in a freeze dryer, crush the freeze-dried sea cucumbers, and store them sealed at 4°C; wash the kelp with clean water, do not use force when cleaning Do not soak in water for too long. After washing, place the kelp in a freeze dryer to freeze-dry, crush the freeze-dried kelp and store it in a sealed container at 4°C.
[0049] 2. Sea cucumber protein separation
[0050] Add ice-distilled water to sea cucumber dry powder according to the ratio of material to water 1:8 (w / v), mix well, adjust the pH to 8, stir several times, rotate at 10000r / min, and centrifuge for 15min to obtain supernatant A and precipitate A.
[0051] Take the supernatant A, adjust the pH to 4, stir at a low speed for 15 minutes at a speed of 10000 r / min, and centrifuge for 15 minutes to obtain the supernatant B and precipitate B. Precipitation B is sea cucumber protein.
[0052] 3...
Embodiment 2
[0059] 1. Raw material processing of sea cucumber and kelp
[0060] Wash fresh sea cucumbers with clean water, freeze-dry them in a freeze dryer, crush the freeze-dried sea cucumbers, and store them sealed at 4°C; wash the kelp with clean water, do not use force when cleaning Do not soak in water for too long. After washing, place the kelp in a freeze dryer to freeze-dry, crush the freeze-dried kelp and store it in a sealed container at 4°C.
[0061] 2. Sea cucumber protein separation
[0062] Add ice-distilled water to sea cucumber dry powder according to the material-to-water ratio of 1:7 (w / v), mix well, adjust the pH to 11, stir several times at 8000r / min, and centrifuge for 20min to obtain supernatant A and precipitate A.
[0063] Take the supernatant A, adjust the pH to 6.5, stir at a low speed for 15 minutes at a speed of 12000 r / min, and centrifuge for 10 minutes to obtain the supernatant B and precipitate B. Precipitation B is sea cucumber protein.
[0064] 3. Sea ...
Embodiment 3
[0071] 1. Raw material processing of sea cucumber and kelp
[0072] Wash fresh sea cucumbers with clean water, freeze-dry them in a freeze dryer, crush the freeze-dried sea cucumbers, and store them sealed at 4°C; wash the kelp with clean water, do not use force when cleaning Do not soak in water for too long. After washing, place the kelp in a freeze dryer to freeze-dry, crush the freeze-dried kelp and store it in a sealed container at 4°C.
[0073] 2. Sea cucumber protein separation
[0074] Add ice-distilled water to sea cucumber dry powder according to the ratio of material to water 1:9 (w / v), mix well, adjust the pH to 10, stir several times at a speed of 10000r / min, and centrifuge for 15min to obtain supernatant A and precipitate A.
[0075] Take the supernatant A, adjust the pH to 5, stir at a low speed for 15 minutes at a speed of 10,000 r / min, and centrifuge for 15 minutes to obtain the supernatant B and precipitate B. Precipitation B is sea cucumber protein.
[00...
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