A method for extracting prunus padus L. anthocyanin from prunus padus L. pericarp
A technique for thick plum anthocyanins and thick plums is applied in the field of preparing thick plum anthocyanins, and can solve the problems of time-consuming, insignificant improvement in product purity, and inability to industrialize production.
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Embodiment 1
[0018] 1000kg of thick plums and 5000kg of extraction solvent alkaline water (pH=12) are dropped into the extraction tank, and 5kg of vitamin C with a purity of 95% is dropped into the extraction tank. The extraction temperature is 70°C, and the extraction time is 90min. Collect the extract; adjust the collected extract with an acidic aqueous solution (pH=1) to make the pH 6.4, then add ZTC1+1 type flocculant therein, with an addition of 2%, at a temperature of 40°C Stir for 5min under the condition of the centrifuge, then let it stand for 120min, centrifuge to collect the centrifugate; purify the centrifugate with 001 type cation exchange resin, the column flow rate of the centrifugate is 1BV / h, and then rinse with deionized water, the flow rate is 1.5BV / h, the consumption of deionized water is 2BV, and then eluted with 4.5% hydrochloric acid aqueous solution, the flow rate of eluent is 1.5BV / h, and the consumption of eluent is 5BV, obtains the wash containing anthocyanin deh...
Embodiment 2
[0020] 1000kg of thick plums and 5000kg of extraction solvent alkaline water (pH=11) are dropped into the extraction tank, and 10 kg of vitamin E with a purity of 50% is dropped into the extraction tank. The extraction temperature is 75° C., and the extraction time is 120 min. Collect the extract; adjust the collected extract with an acidic aqueous solution (pH=1) to make the pH 6.7, then add ZTC1+1 type flocculant therein, with an addition of 2%, at a temperature of 40°C Stir for 5min under certain conditions, then let stand for 120min, centrifuge to collect the centrifugate; purify the centrifugate with D-85 macroporous propionate series weakly acidic cation exchange resin, and the column flow rate of the centrifugate is 1BV / h , then rinse with deionized water, the flow rate is 1.5BV / h, the amount of deionized water is 2BV, and then eluted with 3.0% aqueous hydrochloric acid solution, the flow rate of the eluent is 1.5BV / h, the amount of eluent For 4.5BV, an eluate containin...
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