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A method for enzyme-catalyzed organic chemical reactions in redox stimulus-responsive pickering emulsions

A stimuli-responsive, emulsion technology, applied in the field of physical chemistry, can solve the problems of low recycling rate, difficult enzyme recovery, low reaction efficiency, etc., and achieve the effect of enhancing contact opportunities

Active Publication Date: 2020-11-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to use a kind of Pickering emulsion with redox response performance as the reaction medium of lipase to catalyze the organic / water two-phase reaction in order to solve the problems of low efficiency of conventional enzyme catalyzed reaction, difficult enzyme recovery and low recycling rate.

Method used

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  • A method for enzyme-catalyzed organic chemical reactions in redox stimulus-responsive pickering emulsions
  • A method for enzyme-catalyzed organic chemical reactions in redox stimulus-responsive pickering emulsions
  • A method for enzyme-catalyzed organic chemical reactions in redox stimulus-responsive pickering emulsions

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Example 1: Nano-SiO 2 Pickering emulsion prepared jointly by granules, FTMA and lipase. In a series of cylindrical flasks with a volume of 25mL, accurately weigh nano-SiO with a mass fraction of 0.5% (relative to the aqueous phase) 2 , add 7 mL of FTMA solution of different concentrations. Use an ultrasonic disperser to disperse the particles (90W, 15s). After the nanoparticles are uniformly dispersed, add a certain amount of lipase solution (the final concentration of lipase in the water phase is 1mg·mL -1 ) and 7mL of isooctane, emulsified with a homogenizer (IKA ULTRA-TURRAX T18basic) for 2min at a homogenization rate of 11000rpm to obtain an O / W Pickering emulsion, and the appearance of the emulsion was taken after standing, as shown in figure 2 and image 3 shown.

Embodiment 2

[0023] Example 2: Pickering emulsion prepared jointly by nano calcium carbonate particles, FTMA and reductase. Take 7mL of a series of surfactant aqueous solutions and put them into 25mL columnar vials, add 0.03g of commercial nano-calcium carbonate particles (primary particle diameter is about 10nm, BET specific surface area is 250m 2 / g), use an ultrasonic disperser to disperse the particles (50W, 1min), add 7mL of n-heptane and a certain amount of reductase solution (the final concentration of reductase in the aqueous phase is 1mg·mL -1 ), and emulsified for 2 minutes with a high-shear emulsifier to obtain a stable O / W Pickering emulsion.

Embodiment 3

[0024] Example 3: Pickering emulsion jointly prepared by nano-titanium dioxide particles, FTMA and transferase. Take 7mL of surfactant aqueous solutions with a series of concentrations and put them into 25mL columnar vials, add 0.03g of commercial nano-titanium dioxide particles (primary particle diameter is about 20nm, BET specific surface area is 300m 2 / g), use an ultrasonic disperser to disperse the particles (50W, 1min), add 7mL of isooctane and a certain amount of transferase solution (the final concentration of reductase in the aqueous phase is 1.5mg·mL -1 ), and emulsified for 2 minutes with a high-shear emulsifier to obtain a stable O / W Pickering emulsion.

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Abstract

The invention discloses an enzyme catalyzed organic chemical reaction in a redox stimulus responsive Pickering emulsion. The method is characterized in that the emulsion system, enzyme is loaded to an emulsion interface, thus greatly enhancing the contact opportunity of a reaction substrate and lipase, and promoting efficient reaction; and stirring is unnecessary during reaction, standing reaction is carried out, at the end of reaction, the emulsion is energized for oxidation or trace oxidizing agent is added into the emulsion, the emulsion can achieve controllable separation, and also can realize recycling of the surfactant, nanoparticles and enzyme.

Description

technical field [0001] The invention relates to an enzyme-catalyzed organic reaction in a redox stimulus-responsive Pickering emulsion, belonging to the field of physical chemistry. Background technique [0002] Enzyme is a kind of important catalyst with biological activity. At present, the research on enzyme-catalyzed organic synthesis reaction is relatively common. However, conventional enzyme-catalyzed reactions are carried out in pure organic solvents or organic / water two-phase systems. Organic solvents are directly in contact with enzymes, resulting in decreased enzyme activity, difficult enzyme recovery, and low recycling rates. At the same time, the interaction between enzymes and reaction substrates The limited contact leads to low conversion rate of the reaction, which greatly limits the industrial application of enzymes in catalyzing organic synthesis reactions. [0003] Pickering emulsion is a dispersion system formed by two immiscible liquids (such as oil and w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12P7/40
CPCC12P7/40C12P7/6418
Inventor 蒋建中张杜炎李钰
Owner JIANGNAN UNIV
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