Method for extracting protein and dietary fibers from tea leaves

A dietary fiber and extraction method technology, which is applied in the field of extraction of protein and dietary fiber in tea, can solve the problems of unutilized tea resources, low price, and high labor cost for picking, so as to improve the purity of protein and improve the extraction Efficiency, efficiency and simplicity

Active Publication Date: 2017-12-15
湖南省玉友林农业开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the low-grade tea summer and autumn teas are left unused due to low prices and high picking labor costs.

Method used

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  • Method for extracting protein and dietary fibers from tea leaves
  • Method for extracting protein and dietary fibers from tea leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Take the tea leaves, wash them, drain them, crush them, mix them evenly with 0.05 mol / L Tris-hydrochloric acid buffer solution at a mass ratio of 1:3, place them at 3°C ​​for stirring and extraction for 12 hours, and filter to obtain the filter residue and Extraction solution;

[0017] (2) Add 0.3% NaCl solution to the filter residue, the ratio of solid to liquid is 1:12, ultrasonic extraction at 80W, 50KHZ for 1-2h, the temperature is 15°C, after extraction, let it stand for 30min, then add alkaline protease , the enzymolysis pH is 8.0, the temperature is 49°C, the enzymolysis time is 5 hours, and the enzyme-substrate weight ratio is 1:25; after filtration, the filter residue and filtrate are obtained, after the filtrate is centrifuged, the supernatant is taken, and the supernatant is Place in 4°C, slowly pre-cooled absolute ethanol while stirring, so that the final concentration of ethanol reaches 20%, let it stand for 4 hours, and centrifuge at 10,000 g for 15 mi...

Embodiment 2

[0023] (1) Take the tea leaves, wash them, drain them, crush them, mix them evenly with a buffer containing 0.05 mol / L Tris-hydrochloric acid at a mass ratio of 1:2, place them at 1°C for stirring and extraction for 12 hours, and filter to obtain the filter residue and Extraction solution;

[0024] (2) Add 0.3% NaCl solution to the filter residue, the ratio of solid to liquid is 1:10, ultrasonic extraction at 50W, 40KHZKHZ for 1h, the temperature is 10°C, after extraction, stand for 30min, then add alkaline protease, enzyme The hydrolysis pH is 8.0, the temperature is 49°C, the enzymatic hydrolysis time is 5 hours, and the enzyme-substrate weight ratio is 1:25; after filtration, the filter residue and filtrate are obtained. After the filtrate is centrifuged, the supernatant is taken, and the supernatant is placed in At 4°C, slowly pre-cool absolute ethanol while stirring, so that the final concentration of ethanol reaches 20%, let it stand for 4 hours, and centrifuge at 10,000...

Embodiment 3

[0030] (1) Take the tea leaves, wash them, drain them, crush them, mix them evenly with a buffer solution containing 0.05 mol / L Tris-hydrochloric acid at a mass ratio of 1:4, place them at 4°C for stirring and leaching for 12 hours, and filter to obtain the filter residue and Extraction solution;

[0031] (2) Add 0.3% NaCl solution to the filter residue, the ratio of solid to liquid is 1: 15, ultrasonically extract for 1 hour at 100W, 460KHZ, the temperature is 20°C, let stand for 30 minutes after extraction, then add alkaline protease, enzyme The hydrolysis pH is 8.0, the temperature is 49°C, the enzymatic hydrolysis time is 5 hours, and the enzyme-substrate weight ratio is 1:25; after filtration, the filter residue and filtrate are obtained. After the filtrate is centrifuged, the supernatant is taken, and the supernatant is placed in At 4°C, slowly pre-cool absolute ethanol while stirring, so that the final concentration of ethanol reaches 20%, let it stand for 4 hours, and ...

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Abstract

The invention provides a method for extracting protein and dietary fibers from tea leaves. The protein and the dietary fibers are prepared in one step through subtractive extraction, enzymatic extraction and other methods. The method provided by the invention uses tea leaves as raw materials, extracts the tea protein and the dietary fibers under the assistance of the ultrasonic technique, and has advantages of high extraction rate of protein and fibers, high purity, low cost, simple and easy implementation, and easy popularization; the quality of products meets food hygiene standards; the invention provides a new way for value-added transformation and reuse of the tea leaves, develops a novel plant-derived protein and dietary fiber product, and realizes comprehensive utilization and intensive processing of tea leaf resources.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting protein and dietary fiber in tea. Background technique [0002] my country is a big country of tea production, the tea industry is developing rapidly, the area and output of tea gardens are constantly increasing, and there is a trend of oversupply of tea resources. According to FAO-IGG statistics, the global tea production in 2009 reached 3.9284 million tons. In the past 10 years, China's tea output has continued to grow. From 2000 to 2007, the tea output increased from 719,300 tons to 1.14 million tons, with an average annual growth rate of nearly 11%. In 2009, it reached 1.31 million tons, making it the largest tea producer in the world. country. The tea consumption increased from 456,000 tons in 2000 to 660,000 tons in 2006, with an average annual growth rate of less than 6%. Most of the low-grade tea summer and autumn teas are kept unused du...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C12P19/04A21D13/064A21D2/36A21D2/26
CPCA21D2/264A21D2/36A21D2/364A21D13/064C07K1/145C12P19/04C12P21/06
Inventor 张可池
Owner 湖南省玉友林农业开发有限公司
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