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A rapid method for detecting cold resistance of potato

A potato and ability technology, which is applied in the field of rapid detection of potato cold resistance, can solve the problems of high experimental cost, high scientific research level requirements, complicated experimental process, etc., and achieves good effect.

Active Publication Date: 2020-01-17
INST OF GARDENING ANHUI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The former method can only be tested during the potato planting season and cannot be carried out on an annual basis, and the premise of obtaining results is that the low temperature frost has already adversely affected potato production
The latter method requires a high level of scientific research in the laboratory, the experimental process is complicated, the experimental period is long, and the experimental cost is high
And, at present, the determination method of soluble sugar content in potato leaves mainly adopts the traditional analysis method for measuring materials such as fruits and sugar beets with high sugar content, because the soluble sugar content in potato leaves is relatively low, and some pigments in potato leaves Many, the method used in the analysis of soluble sugar content in potato leaves often causes large errors, and is not suitable for the analysis and determination of soluble sugar content in potato leaves

Method used

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  • A rapid method for detecting cold resistance of potato
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Experimental program
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Effect test

Embodiment 1

[0043] The virus-free test-tube seedlings of the potato variety 'Feiwu Ruita' that had been cultivated for 20 days were transplanted into the substrate (the cultivation substrate was 1:1 nutrient soil and peat soil) and cultivated for 30 days; the potato seedlings cultivated for 30 days were placed in 25 Homogenized treatment in the artificial climate incubator at ℃ for 7 days; the potato seedlings after homogenization treatment were placed in the artificial climate incubator at 0 °C for low temperature treatment, and 3 leaves were collected from the potato seedlings at 0h, 4h, and 12h respectively; each treatment time Take 3 parts of 0.1g leaves to be tested, freeze at -20°C for 1-2 hours, take out the leaves and homogenize them with a high-speed homogenizer at a speed of 18000r / min for 2 minutes to obtain a homogenate of potato leaf tissue; Add distilled water to the homogenate to a total volume of 30ml, put it in a boiling water bath and extract for 30 minutes, take out the ...

Embodiment 2

[0044] The virus-free test-tube seedlings of the potato variety 'Zhongshu 20' cultivated for 20 days were transplanted into the substrate (the cultivation substrate is 1:1 nutrient soil and peat soil) and cultivated for 30 days; the potato seedlings cultivated for 30 days were placed in 25 ° C The homogenized treatment was carried out in the artificial climate incubator for 7 days; after the homogenized treatment, the potato seedlings were placed in the artificial climate incubator at 0°C for low temperature treatment, and 3 leaves of the potato seedlings were collected at 0h, 4h, and 12h respectively; Three parts of 0.1g leaves to be tested were frozen at -20°C for 1-2 hours, and the leaves were taken out and homogenized with a high-speed homogenizer at a speed of 18000r / min for 2 minutes to obtain a homogenate of potato leaf tissue; Add distilled water to the slurry to a total volume of 30ml, put it in a boiling water bath for extraction for 30 minutes, take out the extract and...

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Abstract

The invention discloses a method for quickly detecting the cold resistance ability of potatoes. The steps include freezing the detected potato leaves, then homogenizing and pulverizing, stirring and extracting in a boiling water bath, centrifuging the supernatant, decolorizing with activated carbon, and filtering to obtain the extraction. Then add concentrated sulfuric acid directly to the extract for acidification treatment, then add ethyl anthrone acetate, shake and mix repeatedly, put it in a boiling water bath to keep warm for reaction, then take it out and quickly put it in an ice bath to terminate the reaction, and finally use UV spectrophotometer measures the absorbance at 639nm, and converts the soluble sugar content in potato leaves. By comparing the soluble sugar content in potato leaves of different varieties and different treatment times, the cold resistance ability of different varieties of potatoes can be obtained. The invention provides an analysis method with correct analysis result, fast analysis speed, simple operation, high sensitivity and good reproducibility for measuring the cold resistance ability of potatoes.

Description

technical field [0001] The invention relates to a method for rapidly detecting the cold resistance ability of potatoes. Background technique [0002] Anhui Province and other potato central plains secondary cropping areas use paddy winter slack fields to grow potatoes. Potatoes grown in this area often encounter low temperature and frost climates such as late spring and cold, and low temperature is extremely harmful to potatoes. The cold resistance of different varieties of potato is not the same, and its cold resistance is positively correlated with the change of soluble sugar in potato leaves in response to low temperature. Therefore, there is a need for an experimental method that can be carried out quickly, easily and accurately to detect the corresponding low temperature-induced changes in soluble sugars in potato leaves. [0003] At present, the detection of potato cold resistance is mainly based on two methods: observing the frostbite of potato leaves cultivated in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33G01N1/28
CPCG01N1/28G01N21/33
Inventor 闫冲冲廖华俊江芹宁志怨杨月英王前前
Owner INST OF GARDENING ANHUI ACADEMY OF AGRI SCI