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Detection method of water body genetic toxicity of urban drinking water

A genotoxicity and detection method technology, applied in the field of detection and analysis, can solve the problems of high long-term maintenance cost of experimental animals, low sensitivity or positive accuracy ratio, time-consuming long-term test methods, etc. time consuming effect

Inactive Publication Date: 2017-12-15
SHANDONG WATER & WASTEWATER MONITORING CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] There are many kinds of organic chemical substances in drinking water and their concentrations are low, which requires high sensitivity and precision of the analysis method, and the toxicological effects of organic harmful substances are chronic, and the toxicity to the human body is mostly complex; genotoxicity analysis is due to The shortcomings of long-term testing methods such as time-consuming and expensive long-term maintenance of experimental animals have been replaced by fast and cheap short-term screening tools. The sensitivity of the method is 100%. Therefore, it is recommended to combine several short-term tests at home and abroad, that is, battery test, and take into account different cell types (prokaryotic cells and eukaryotic cells) and different observation endpoints (gene mutation and chromosome test). Distortion) (Bajpayee et al., 2005) for comprehensive biological toxicity testing for effective drinking water safety assessment

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  • Detection method of water body genetic toxicity of urban drinking water
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Examples

Experimental program
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Effect test

Embodiment 1

[0024] (1) adopt detection step of the present invention:

[0025] (1) First wash the 4021 reverse osmosis membrane with pure water at a washing speed of 8L / min; take 100L of a surface source water, and during reverse osmosis treatment, the water sample is processed at a speed of 80-100mL / min for reverse osmosis treatment. The pressure is 0.1MPa, and 3.8L of reverse osmosis concentrated water is obtained;

[0026] (2) Collect 3.8 L of concentrated water obtained by reverse osmosis, filter it through a 0.45 μm glass cellulose membrane, and then enrich it with an HLB column. Use 5mL of n-hexane / dichloromethane (1:1, volume ratio) and 10mL methanol / dichloromethane (1:9, volume ratio) as eluents for elution. Dry the eluent and replace the solvent with DMSO, set the volume to 5mL to obtain the sample to be tested, and store it at -20°C;

[0027] (3) The samples to be tested are subjected to genotoxicity experiments

[0028] ①Ames experiment: The plate incorporation method was us...

Embodiment 2

[0043] This specific embodiment adopts the following steps:

[0044] (1) First wash the 4021 reverse osmosis membrane with pure water at a washing speed of 8L / min; take 100L of factory water from a certain water plant, and during reverse osmosis treatment, the water sample is processed at a rate of 80-100mL / min for reverse osmosis treatment. The pressure of the membrane is 0.1MPa, and 3.8L of concentrated reverse osmosis water is obtained;

[0045] (2) Collect 3.8 L of concentrated water obtained by reverse osmosis, filter it through a 0.45 μm glass cellulose membrane, and then enrich it with an HLB column. Use 5mL of n-hexane / dichloromethane (1:1, volume ratio) and 10mL methanol / dichloromethane (1:9, volume ratio) as eluents for elution. Dry the eluent and replace the solvent with DMSO, set the volume to 5mL to obtain the sample to be tested, and store it at -20°C;

[0046] (3) The samples to be tested are subjected to genotoxicity experiments

[0047] ①Ames experiment: Th...

Embodiment 3

[0052] This specific embodiment adopts the following steps:

[0053] (1) First wash the 4021 reverse osmosis membrane with pure water at a rate of 8L / min; take 100L of water from a certain pipe network, and during reverse osmosis treatment, feed water samples at a rate of 80-100mL / min for reverse osmosis treatment. The pressure is 0.1MPa, and 3.8L of reverse osmosis concentrated water is obtained;

[0054] (2) Collect 3.8 L of concentrated water obtained by reverse osmosis, filter it through a 0.45 μm glass cellulose membrane, and then enrich it with an HLB column. Use 5mL of n-hexane / dichloromethane (1:1, volume ratio) and 10mL methanol / dichloromethane (1:9, volume ratio) as eluents for elution. Dry the eluent and replace the solvent with DMSO, set the volume to 5mL to obtain the sample to be tested, and store it at -20°C;

[0055] (3) Recombinant Escherichia coli-SOS effect experiment was carried out on the sample to be tested: After the preserved recombinant Escherichia c...

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Abstract

The invention discloses a detection method of water body genetic toxicity of urban drinking water. The detection method comprises the steps of treating a water sample with a reverse osmosis technology; performing solid phase extraction on a condensed water sample subjected to reverse osmosis treatment; and performing Ames experiment and recombinant E. coli-SOS effect and comet experiments, so as to obtain equivalent concentration of the genetic toxicity effect. According to the method of measuring water body genetic toxicity through reverse osmosis-solid phase extraction-grouped genetic toxicity experiments, the water body genetic toxicity can be evaluated comprehensively, and the problems of complex pre-treatment, large use amount of an organic reagent in the water body genetic toxicity measurement and the like are solved. The method is convenient to operate, consumes less time, and has high detection sensitivity and low operation risk.

Description

technical field [0001] The invention relates to a method for measuring genotoxicity in water bodies, in particular to a method for detecting genotoxicity in urban drinking water, and belongs to the field of detection and analysis. Background technique [0002] Most trace organic substances in water have the properties of long-term persistence, bioaccumulation, semi-volatility and high toxicity. These organic substances enter the environment in large quantities with human activities, and concentrate and amplify along the food chain, causing harm to human health. Although the concentration of most organic substances in water is very low, most organic substances are difficult to degrade and bioaccumulate. Through their migration, transformation and enrichment in the environment, the concentration level can be increased several times or even hundreds of times, which is very important for long-term drinking. cause great potential harm. Organic pollutants are often present in lo...

Claims

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Application Information

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IPC IPC(8): G01N33/18G01N1/40
CPCG01N33/1866G01N1/40
Inventor 贾瑞宝孙韶华逯南南许燕孙莉
Owner SHANDONG WATER & WASTEWATER MONITORING CENT
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