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Method for extracting small molecule peptide from cubilose

A technology of small molecular peptides and bird's nests, applied in the field of protein peptides, can solve the problems of waste, low peptide absorption efficiency, affecting the edible effect, etc., and achieve the effect of small molecular weight

Inactive Publication Date: 2017-12-19
广西佛斯肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, when the bird's nest is eaten by ordinary eating methods, it has not been treated by breaking the wall, and the absorption efficiency of the internal polypeptide is not high, which affects the eating effect and causes waste
[0007] In-depth development of bird's nest, targeted fine separation and extraction of high-quality small molecular peptides and other beneficial ingredients from bird's nest, by breaking the wall of bird's nest molecules, the protein in bird's nest is released, and then a soluble protein is formed through a specific enzymatic method There is no relevant report on the technology of bird's nest small molecule peptide in Yushui

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for extracting small molecular peptides from bird's nest, comprising the following steps:

[0024] (1) The bird’s nest is dried in the sun, crushed into primary powder, and the primary powder is moved to a drying oven for 6 hours at low temperature at a drying temperature of 58°C;

[0025] (2) The primary powder is broken through a wall breaking machine, the breaking condition is 10000r / min, and the broken wall forms a powder until it can pass through a 100-mesh sieve to obtain a fine powder;

[0026] (3) The fine powder: ion-free water mass ratio is 1:18 to form a mixed solution, put it into a heating tank, heat the mixed solution to 100°C for sterilization for 8 minutes and let it cool down to below 50°C;

[0027] (4) Put the mixture obtained in the above steps into an ultrasonic water bath for oscillation, the temperature range is 40°C, the oscillation time is 12 hours, and NaHCO is added to the mixture 3 Solution adjustment pH adjustment = 10.2;

[0028] ...

Embodiment 2

[0035] A method for extracting small molecule peptides from bird's nest, comprising the following steps:

[0036] (1) The bird’s nest is dried in the sun, crushed into primary powder, and the primary powder is moved to a drying oven for low-temperature drying for 8 hours at a drying temperature of 55°C;

[0037] (2) Break the primary powder through a wall breaking machine, the breaking condition is 10000r / min, and the broken wall forms a powder until it can pass through a 100-mesh sieve to obtain a fine powder;

[0038] (3) The fine powder: ion-free water mass ratio is 1:18 to form a mixed solution, put it into a heating tank, heat the mixed solution to 100°C for sterilization for 8 minutes and let it cool down to below 50°C;

[0039] (4) Put the mixture obtained in the above steps into an ultrasonic water bath for oscillation, the temperature range is 44°C, the oscillation time is 9 hours, and NaHCO is added to the mixture 3 Solution adjustment pH adjustment = 10.2;

[0040...

Embodiment 3

[0047] A method for extracting small molecular peptides from bird's nest, comprising the following steps:

[0048] (1) The bird’s nest is dried in the sun, crushed into primary powder, and the primary powder is moved to a drying oven for 12 hours at a low temperature, and the drying temperature is 50°C;

[0049] (2) The primary powder is broken through a wall breaking machine, the breaking condition is 10000r / min, and the broken wall forms a powder until it can pass through a 100-mesh sieve to obtain a fine powder;

[0050] (3) The fine powder: ion-free water mass ratio is 1:18 to form a mixed solution, put it into a heating tank, heat the mixed solution to 100°C for sterilization for 8 minutes and let it cool down to below 50°C;

[0051] (4) Put the mixture obtained in the above steps into an ultrasonic water bath for oscillation, the temperature range is 50°C, the oscillation time is 4 hours, and NaHCO is added to the mixture 3 Solution adjustment pH adjustment = 10.2;

[...

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PUM

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Abstract

The invention discloses a method for extracting a small molecule peptide from cubilose. The method disclosed by the invention comprises the following steps: pulverizing sun-dried cubilose into powder, adding deionized water to the powder to prepare a mixture by blending, putting the mixture into an ultrasonic water bath kettle, carrying out electromagnetic heating for enzymolysis in an alkaline environment after oscillation, separating the mixture by using a centrifugal machine provided with a centrifugal roller with three layers of filtering membranes to obtain a fine filtrate, placing the fine filtrate in a freezer for cryogenic stabilization, and moving the fine filtrate into a vacuum drier for drying to obtain the small molecule peptide extracted from the cubilose. Through the steps, a freely soluble cubilose small molecule peptide can be separated from the cubilose, so as to improve the absorption capacity of a human body for the cubilose small molecule peptide.

Description

technical field [0001] The present invention relates to the field of protein polypeptides, and in particular to a method for extracting small molecule peptides from bird's nest. Background technique [0002] Bird's nest, also known as swallow dish, swallow root and swallow vegetable, refers to the nest made of the saliva secreted by some swifts of the Swift family Swift family and several swiftlets of the genus Swiftlet, mixed with other substances. [0003] Bird's nest is one of the traditional and precious foods that have been eaten in China since the Ming Dynasty. Modern medical research has found that the main components of bird's nest are: water-soluble protein, carbohydrates; Amino acids that play important roles (lysine, cystine and arginine), of which there is one essential amino acid (lysine) and three conditionally essential amino acids. [0004] Bird’s nest’s unique protein content contains biologically active molecules that help the growth, development and recov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/34C07K1/14
CPCC07K14/78C12P21/06Y02P20/133Y02P20/59
Inventor 不公告发明人
Owner 广西佛斯肽生物科技有限公司