A graphene photoelectric dual detection biosensor, system and method
A biosensor and dual detection technology, applied in the field of analytical chemistry, can solve the problems of single detection, unfavorable DNA hybridization kinetics mechanism, affecting the reliability of results, etc., and achieve the effect of reliable experimental results.
Active Publication Date: 2020-11-20
SHANDONG NORMAL UNIV
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[0004] However, in the past, optical or electrical single detection technology was used to detect DNA in previous biosensors, which not only affects the reliability of the results, but also hinders the in-depth understanding of the kinetic mechanism of DNA hybridization.
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[0033] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
[0034] It should be pointed out that the following detailed description is exemplary and intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
[0035] It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinatio...
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The present invention discloses a graphene photoelectric double detection biosensor, a graphene photoelectric double detection system and a graphene photoelectric double detection method. According to the present invention, a graphene film growing on a copper foil surface by using a chemical vapor deposition method is transferred to a glass substrate with two electrodes through a wet transfer method so as to be used as a channel between the two electrodes, the two electrodes are respectively used as a source electrode and a drain electrode, a reaction chamber prepared from a polydimethylsiloxane material is adhered on the graphene surface through an ultraviolet curing adhesive so as to be used as a container for holding an electrolytic solution and a grid electrode, and a probe is fixed on the graphene by using 1-succinimidyl pyrenebutanoate as a linker; and the FRET biosensor and the field-effect transistor biosensor are combined into the one detection system so as to achieve the photoelectric double real-time detection of DNA hybridization.
Description
technical field [0001] The invention relates to a graphene photoelectric dual detection biosensor, system and method, which realizes real-time dynamic detection of DNA hybridization and belongs to the field of analytical chemistry. Background technique [0002] Graphene is a two-dimensional nanomaterial with a single-layer sheet structure composed of carbon atoms. Due to its unique physical and chemical properties such as large body-to-surface ratio, high electron mobility, excellent thermoelectric conductivity, and high mechanical strength, graphene has gained more and more attention in the development and application of various biosensors. s concern. Biomolecules adsorbed on the surface of graphene can adjust the channel doping of its charge or form a double conductive layer with graphene film, causing the change of graphene conductivity, which makes graphene an ideal conductive channel in field effect tube biosensors The material is widely used in the detection of pH va...
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IPC IPC(8): C12M1/34G01N21/64G01N27/04
CPCC12Q1/6825G01N21/6428G01N27/041G01N2021/6432C12Q2565/607C12Q2563/107
Inventor 岳伟伟汤彩艳谢晓慧华宏令张震李佳宁刘淑一张哲豪
Owner SHANDONG NORMAL UNIV