Micro nanometer biological particle sorting equipment based on micro-fluidic chip

A technology of microfluidic chips and biological particles, applied in biochemical equipment and methods, enzymology/microbiology devices, methods of supporting/immobilizing microorganisms, etc., can solve the problem that the biological activity of exosomes is easily affected by pH and salt concentration , unfavorable downstream experiments and other issues

Active Publication Date: 2017-12-29
北京旌准医疗科技有限公司
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  • Abstract
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Problems solved by technology

However, limited by specific markers, the biological activity of exosomes is easily affected by pH and salt concentration, which is not conducive to downstream experiments

Method used

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  • Micro nanometer biological particle sorting equipment based on micro-fluidic chip
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  • Micro nanometer biological particle sorting equipment based on micro-fluidic chip

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Embodiment Construction

[0032] The following is attached Figure 1-2 The present invention is described in further detail.

[0033] Such as Figure 1-2 As shown, the micro-nano biological particle sorting device based on the microfluidic chip of the present invention includes a PMMA cover plate 1 , a PDMS chip 4 , a micro-nano filter membrane 5 and a PMMA base 7 . Among them, the PDMS chip 4 is formed by bonding the upper cover sheet and the lower layer substrate. The surface of the PDMS chip 4 is provided with a serpentine microfluidic channel 6. The height of the microfluidic channel 6 is 0.3 mm, the length is 220 mm, and the width is 1.5 mm. mm, and the interval between each channel in the microfluidic channel 6 is 1mm. The processing of the PDMS chip 4 is formed by pouring the prepared PDMS into a silicon wafer mold (photolithography-etching process) and heating and curing. The PMMA cover plate 1 and the PMMA base 7 are processed by an engraving machine to support and fasten them. The dimensio...

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Abstract

The invention relates to micro nanometer biological particle sorting equipment based on a micro-fluidic chip. The equipment comprises a PMMA cover plate, a base, a PDMS chip and a micro nanometer hole filtering film, wherein the PMMA cover plate and the base fix and tightly press the PDMS chip and the micro nanometer filtering film through screw bolts; the PDMS chip is made of PDMS materials; completely identical snake-shaped micro-fluidic passages are arranged on the surfaces of a cover piece and a base piece; micro nanometer scale filtering holes are densely distributed in the micro nanometer filtering film; the filtering films with different hole diameters can be selected according to requirements; the separation requirements of biological particles in different sizes can be met. The micro nanometer biological particle sorting equipment can realize the fast and efficient sorting collection on the biological particles in different sizes in a clinic liquid sample; the operation is simple, convenient and fast; the automation degree is high; the cost is low. The technology uses the micro nanometer hole filtering film; a physical interception method is used; blood platelets in the whole blood samples are fast separated; by controlling the upper and lower micro cavity pressure and the flowing speed, the diffusion effect of the nanometer scale particles in the vertical direction can be improved, so that exosomes in the sample can be fast and conveniently separated; wide scientific research and clinic values are realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a rapid sorting device for micro- and nano-scale biological particles based on microfluidic technology. Background technique [0002] Microfluidics is a technology to precisely control and manipulate micro-scale fluids. This technology originated in the 1980s and has been rapidly developed and widely used in rapid diagnosis (POCT) and other aspects. Microfluidic chip, also known as "lab-on-a-chip", is the main platform for the realization of microfluidic technology. Through microprocessing technology, functional components such as micropipes, micropumps, microvalves, microreservoirs, and microdetection elements integrated on a single chip. Microfluidic technology has a unique micro-scale effect and utilizes the characteristics of microfluidic mechanics, and has unique advantages in the sorting of biological particles such as cells. This technology can effectively separ...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12M1/00
CPCC12M23/16C12M33/14C12M47/04
Inventor 姚文亮施建春叶锋
Owner 北京旌准医疗科技有限公司
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