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Chitosan nanometer micro-bubble and preparation method and application thereof

A chitosan nanometer and chitosan technology, which is applied in pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of poor imaging effect, complicated preparation process of nano-microbubbles, and high cost. higher question

Active Publication Date: 2018-01-05
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above-mentioned shortcoming of the prior art, the object of the present invention is to provide a kind of chitosan nano-microbubble and its preparation method and application, for solving the complicated preparation process of the nano-microbubble in the prior art, higher cost, Problems such as poor imaging effect

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  • Chitosan nanometer micro-bubble and preparation method and application thereof
  • Chitosan nanometer micro-bubble and preparation method and application thereof
  • Chitosan nanometer micro-bubble and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0052] A preparation method of chitosan nano microbubbles, comprising the steps of:

[0053](1) Prepare 50 mL of acrylic acid aqueous solution with a mass concentration of 0.44%, add chitosan, the chitosan mass (unit g) added is 1% of the volume (unit mL) of the acrylic acid aqueous solution, stir until completely dissolved, and heat under nitrogen protection To 75°C, add 70 mg of potassium persulfate, react for about 1 hour, set the temperature to 60°C, continue the reaction for 80 minutes, then filter the product to remove impurities, then pour it into a dialysis bag, and then place it in acetic acid with pH=4.5- In sodium acetate buffer solution, magnetically stirred for 24 hours, dialyzed to remove impurities, then taken out, added 0.078g of glutaraldehyde, heated to 40°C for reaction, after 2 hours, the reaction was completed, and a nanoparticle suspension was prepared, with a particle size of about 100nm , and has a hollow structure, its shape is observed by transmission...

Embodiment 2

[0056] A preparation method of chitosan nano microbubbles, comprising the steps of:

[0057] (1) Prepare 50 mL of an acrylic acid solution with a mass concentration of 0.22%, add chitosan, the chitosan mass (unit g) added is 0.5% of the volume (unit mL) of the acrylic acid aqueous solution, stir until completely dissolved, and heat under nitrogen protection To 75°C, add 45 mg of potassium persulfate, react for about 1 hour, set the temperature to 60°C, after 100 minutes, filter the product to remove impurities, then pour it into a dialysis bag, and place it in acetic acid-sodium acetate buffer at pH=4.5 In the liquid, take it out after magnetic stirring for 24 hours, add 0.035g of glutaraldehyde, and heat to 40°C for reaction. After 2 hours, the reaction is completed, and the nanoparticle suspension is obtained. The particle size of the nanoparticle is about 100nm and has a hollow structure. form and figure 1 similar.

[0058] (2) Add lactose to the nanoparticle suspension p...

Embodiment 3

[0060] A preparation method of chitosan nano microbubbles, comprising the steps of:

[0061] (1) Prepare 50 mL of acrylic acid solution with a mass concentration of 0.44%, add chitosan, the quality of chitosan added (in g) is 1% of the volume of acrylic acid aqueous solution (in mL), heat to 70°C under nitrogen protection, add 60mg of potassium persulfate, react for about 1 hour, set the temperature to 60°C, filter the product after 80 minutes to remove impurities, then pour it into a dialysis bag, put it in acetic acid-sodium acetate buffer solution with pH=4.5, and stir it magnetically for 24 hours Take it out, add 0.078g of glutaraldehyde, heat to 40°C for reaction, after 2h, the reaction is complete, and a nanoparticle suspension is obtained. The particle size of the nanoparticle is about 100nm and has a hollow structure. figure 1 similar.

[0062] (2) Add mannitol to the nanoparticle suspension prepared in step (1), the quality (unit g) of mannitol is 5% of the volume (u...

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Abstract

The invention provides a chitosan nanometer micro-bubble and a preparation method and application thereof. The method comprises the steps of 1, preparing an acrylic acid aqueous solution, adding chitosan, heating the solution to 60-80 DEG C in the presence of protective gas after dissolution, adding an initiator for a reaction, then reducing the temperature of the solution for a reaction, conducting filtration and dialysis to remove impurities after the reaction is finished, adding a cross-linking agent for a reaction after processing is finished, conducting heating, and obtaining a nano-particle suspension after the reaction; 2, adding a freeze-drying protective agent into the nano-particle suspension, conducting freeze drying, and obtaining the chitosan nanometer micro-bubble. Accordingthe method, the chitosan is dissolved in the acrylic acid solution according to a certain proportion, the initiator is added to make acrylic acid and chitosan subjected to a polymerization reaction toobtain hollow nanoparticles internally containing water, water of the internal hollow structures is removed, and the obtained nanometer micro-bubble after being dissolved again has the synergistic effects on ultrasonic development and high-intensity focused ultrasonic tissue ablation.

Description

technical field [0001] The invention relates to the field of contrast agents, in particular to a chitosan nano-microbubble and its preparation method and application. Background technique [0002] At present, the more mature ultrasound contrast agents are micron-scale contrast agents, with a diameter range of about 2-8 μm. They can pass through the pulmonary capillaries smoothly without causing embolism, but they cannot penetrate outside the blood vessels, which limits their ability to visualize extravascular diseases. Moreover, the blood vessels at the site of inflammation and embolism are also narrow, and sometimes micron-sized ultrasound contrast agents cannot pass through smoothly. With the development of molecular biology and nanotechnology, nanopharmaceuticals are rapidly emerging. The particle size of nano-scale contrast agents is often within 700nm, which can effectively realize extravascular imaging, and has more advantages than micron-scale microbubbles in clinica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K31/704A61K9/16A61K47/36A61K47/32A61P35/00
Inventor 余超群杨增涛
Owner CHONGQING MEDICAL UNIVERSITY
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