Microfluidic chip device for detecting various tumor markers by using fifteen channels in combined manner

Abstract

The invention relates to a microfluidic chip device for detecting various tumor markers (TMs) by using fifteen channels in a combined manner, and belongs to the field of analysis and testing. When polydimethylsiloxane (PDMS) is used to manufacture a substrate of a universal microfluidic chip used for combined detection of more than ten tumor markers, a plurality of difficult problems need to be overcome. Key points of the scheme are as follows: PDMS with an original ecological surface is used as a substrate material, and the interiors of pipelines of the chip are filled with doped micron-sizedtitanium dioxide particles; the titanium dioxide particles have super-hydrophilic surfaces, and the super-hydrophilic surfaces can be formed by inducing of preliminary visible light irradiation; aggregation of super-hydrophilic gap channels among the particles of the titanium dioxide particle filler replaces, compensates and exceeds original strong-hydrophobic pipelines, and therefore constant and super-hydrophilic modification effects are achieved; and a difference of interfacial tension is formed between head and tail ends of the chip by inducing of micro-power supersonic waves, and therefore sample liquid flows are guided to flow towards a tail-end direction of the chip along the pipelines of the chip.

Description

technical field [0001] The invention relates to a microfluidic chip device for joint detection of multiple tumor markers with fifteen channels, belonging to the field of analysis and testing. Background technique [0002] Tumor markers (tumor markers, TM) refer to a class of substances produced by tumor cells themselves or produced by the body's response to tumor cells during the occurrence and proliferation of tumors, reflecting the existence and growth of tumors, including Proteins, hormones, enzymes (isoenzymes) and oncogene products, etc. Testing the tumor markers in the patient's blood or body fluids can detect tumors early in the tumor screening, observe the curative effect of tumor treatment and judge the prognosis of patients. Currently, the tumor markers commonly used clinically are: (1) alpha-fetoprotein (AFP) is a marker for primary liver cancer, testicular cancer, ovarian cancer and other tumors; (2) carcinoembryonic antigen (CEA) is a marker for digestive syste...

AI Technical Summary

Problems solved by technology

[0008] But it's not that simple

[0009] First, this polydimethylsiloxane material, which is referred to by the acronym PDMS, is itself a strongly hydrophobic material, and channels are built on this material. modification operation, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the channel in the structure occupies most of the inner surface of the liquid flow channel, then the inner surface of the PDMS channel is The strong hydrophobicity is the decisive factor, it will make it very difficult for the polar liquid fine flow similar to the aqueous solution to pass through, and its flow resistance is so large that even the general micropump is difficult to push, of course, if the cover sheet is also If you choose to use the PDMS material, then the problems are basically the same, with minor differences; therefore, in the prior art, it is necessary to modify and modify the inner surface of the channel on the PDMS material; then, this is for the PDMS channel Is the modification operation of the inner surface of the channel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the channel modified by the surface unable to maintain for a long enough time. The maintenance time of the internal surface state is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the surface state of the PDMS channel inner surface after surface modification or surface modification is formed after modification It cannot last for a long time, but it will automatically tend to or change back to the surface state before the surface modification, and return to the original strongly hydrophobic surface state in a short period of time. Then, just imagine, such a slight Can flow control chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible

This problem has also existed for many years, and so far, it has not been properly solved

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