Micro-fluidic chip device for simultaneously detecting various typical female tumor markers

A microfluidic chip and tumor marker technology, which is applied in the field of microfluidic chip devices, can solve the problems of difficulty in passing fine liquid flow, has not been properly solved, and large flow resistance.

Inactive Publication Date: 2018-01-12
李榕生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] But it's not that simple
[0009] First, this polydimethylsiloxane material, which is referred to by the acronym PDMS, is itself a strongly hydrophobic material, and channels are built on this material. modification operation, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the channel in the structure occupies most of the inner surface of the liquid flow channel, then the inner surface of the PDMS channel is The strong hydrophobicity is the decisive factor, it will make it very difficult for the polar liquid fine flow similar to the aqueous solution to pass through, and its flow resistance is so large that even the general micropump is difficult to push, of course, if the cover sheet is also If you choose to use the PDMS material, then the problems are basically the same, with minor differences; therefore, in the prior art, it is necessary to modify and modify the inner surface of the channel on the PDMS material; then, this is for the PDMS channel Is the modification operation of the inner surface of the channel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the channel modified by the surface unable to maintain for a long enough time. The maintenance time of the internal surface state is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the surface state of the PDMS channel inner surface after surface modification or surface modification is formed after modification It cannot last for a long time, but it will automatically tend to or change back to the surface state before the surface modification, and return to the original strongly hydrophobic surface state in a short period of time. Then, just imagine, such a slight Can flow control chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible
This problem has also existed for many years, and so far, it has not been properly solved

Method used

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  • Micro-fluidic chip device for simultaneously detecting various typical female tumor markers

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Embodiment Construction

[0076] exist figure 1In the example shown in this case, the main point of this example is that the structure of the device includes a multi-channel microfluidic chip, and the structure of the microfluidic chip includes a substrate 5 and a cover sheet 6 that are attached to each other and installed together. , the substrate 5 and the cover sheet 6 are both plates or sheets, the surface of the substrate 5 facing the cover sheet 6 contains a channel structure formed by a molding process or an etching process, the substrate 5 also contains a window structure connected to the channel structure and pierced through the substrate 5 through a molding process, an etching process or a simple punching process. The substrate 5 and the cover sheet 6 that are mounted together A microfluidic chip containing a pipeline structure and a liquid pool structure connected thereto is constructed. The structure of the pipeline is located at the interface area where the substrate 5 and the cover sheet ...

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Abstract

The invention relates to a micro-fluidic chip device for simultaneously detecting various typical female tumor markers (TMs), and belongs to the field of analysis and testing. When polydimethylsiloxane (PDMS) is used to manufacture a substrate of a micro-fluidic chip used for combined detection of eight typical tumor markers which are suitable for women, a plurality of difficult points exist. Keypoints of the technical scheme are as follows: PDMS with an original ecological surface is used as a substrate material, and the interiors of pipelines of the chip are filled with doped micron-sized titanium dioxide particles; the titanium dioxide particles have super-hydrophilic surfaces, and the super-hydrophilic surfaces can be formed by inducing of preliminary visible light irradiation; aggregation of super-hydrophilic gap channels among the particles of the titanium dioxide particle filler replaces, compensates and exceeds original strong-hydrophobic pipelines, and therefore constant andsuper-hydrophilic modification effects are achieved; and a difference of interfacial tension is formed between head and tail ends of the chip by inducing of micro-power supersonic waves, and thereforesample liquid flows are guided to flow towards a tail-end direction of the chip along the pipelines of the chip.

Description

technical field [0001] The invention relates to a microfluidic chip device for simultaneously detecting multiple typical tumor markers of women, belonging to the field of analysis and testing. Background technique [0002] Tumor markers (tumor markers, TM) refer to a class of substances produced by tumor cells themselves or produced by the body's response to tumor cells during the occurrence and proliferation of tumors, reflecting the existence and growth of tumors, including Proteins, hormones, enzymes (isoenzymes) and oncogene products, etc. Testing the tumor markers in the patient's blood or body fluids can detect tumors early in the tumor screening, observe the curative effect of tumor treatment and judge the prognosis of patients. Currently, the tumor markers commonly used clinically are: (1) alpha-fetoprotein (AFP) is a marker for primary liver cancer, testicular cancer, ovarian cancer and other tumors; (2) carcinoembryonic antigen (CEA) is a marker for digestive syst...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N33/574
Inventor 李榕生
Owner 李榕生
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