Method for preparing electrochemical sensor used for detecting concentration of galectin-3 in serum

A technology of concentration detection and electrochemistry, applied in the direction of nanotechnology for sensing, electrochemical variables of materials, scientific instruments, etc., can solve problems such as limitations and uneven applications, achieve simple methods, promote development, and facilitate the realization of commercial products the effect of

Active Publication Date: 2018-01-12
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology

However, its application in immunosensor signaling probes is limited due to its heterogeneous morphology

Method used

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  • Method for preparing electrochemical sensor used for detecting concentration of galectin-3 in serum
  • Method for preparing electrochemical sensor used for detecting concentration of galectin-3 in serum
  • Method for preparing electrochemical sensor used for detecting concentration of galectin-3 in serum

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Embodiment 1

[0047] Step 1. Weigh 0.187g FeCl respectively 3 ·6H 2 O and 0.126g of 2-aminoterephthalic acid were added to 15mL of dimethylformamide (DMF) solution, and mixed. The above mixed solution was heated in 120 °C silicone oil for 4 h, and 15 min after the start of heating, 200 μL of glacial acetic acid was added to the mixed solution. After heating, the temperature of the solution was controlled to cool down to room temperature, and the obtained solution was centrifuged at 10,000 r / min for 5 minutes, and washed three times with DMF, absolute ethanol and ultrapure water respectively. The obtained precipitate was dried in vacuum for 24 hours to obtain Fe-MOFs, which were stored at 4°C for future use.

[0048] Step 2. Take 2mL HAuCl 4 4H 2 O (1%) solution was added to the prepared 2mL Fe-MOFs (1mg mL -1 ) solution, after vigorous ultrasonication for 30 minutes, slowly add 4mL NaBH to the above solution dropwise 4 (0.1M) at the same time placed on a magnetic stirrer for stirring ...

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Abstract

The invention relates to a preparation method and application of an electrochemical sensor for detecting a blood concentration level of an insulin sensitivity reduction early diagnosis biomarker, namely galectin-3 (Gal-3), causing type II diabetes, and belongs to the technical field of electrochemical detection. The preparation method is characterized by comprising the following steps: respectively synthesizing an N-GNRs-Fe-MOFs@AuPNs sensor substrate modified nano composite material and an AuPt-MB nano composite signal material, enabling a detection antibody capable of being in specific binding to the Gal-3 to be mixed with the AuPt-MB composite material so as to prepare a biological signal probe; conjugating a Gal-3 specific capture antibody on a substrate platform modified by the N-GNRs-Fe-MOFs@AuPNs composite material so as to conveniently and specifically capture a target Gal-3; finally, performing specific binding on the Gal-3 as well as the detection antibody and the capture antibody, thereby obtaining the electrochemical sensor for detecting the blood concentration level of the Gal-3. The sensor is successfully used for detecting the Gal-3 in serum. The electrochemical sensor disclosed by the invention has the advantages of being high in sensitivity, high in specificity, rapid in detection and convenient. An early diagnostic basis is provided for insulin resistance causing the type II diabetes.

Description

Technical field: [0001] The present invention relates to the preparation method and application of an electrochemical sensor for the detection of the blood concentration level of galectin-3 (Gal-3), a biomarker for early diagnosis of insulin sensitivity reduction induced by type 2 diabetes, especially based on AuPt-MB nanocomposite material as A sandwich-type immune biosensor prepared by a novel signal probe is used for detecting the concentration of galectin-3 (Gal-3) in serum, and belongs to the field of electrochemical detection. Background technique: [0002] Diabetes is rapidly increasing worldwide and poses a serious threat to human health. In particular, type 2 diabetes (T2D), caused by decreased insulin sensitivity, can lead to fatal cardiovascular complications and accounts for 90% of the diabetes prevalence. In a recent study, it was found that galectin-3 (Gal-3) can directly bind to insulin receptors, inhibit downstream insulin receptor (IR) signaling and link in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/48G01N33/543B82Y15/00
Inventor 于超唐志勇陈俊
Owner CHONGQING MEDICAL UNIVERSITY
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