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Microalgae culture method

A technology of microalgae culture and culture solution, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to effectively increase the oil content of microalgae, reduce the greenhouse effect, save access costs, The effect of increasing content and yield

Inactive Publication Date: 2018-01-16
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the present invention proposes a method for cultivating microalgae, aiming at solving the problem that the prior art cannot effectively improve the oil content of microalgae

Method used

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Embodiment 1

[0049] Both the control group 1 and the breeding group 1 inoculated a self-isolated algae species in the logarithmic growth phase in a 5cm optical path tube reactor, the light was 150μmol / ㎡ / s, 24 hours a day light, and the temperature was room temperature , the initial culture solution is the improved BG11 culture solution suitable for microalgae growth induction, and the formula of the improved BG11 culture solution is shown in Attached Table 1.

[0050] Breeding group one is to add 1g / L NaHCO to the initial culture medium 3 , then the bicarbonate ion concentration in the culture medium is about 12mmol / L. Introduce algae species into the prepared culture medium and start the cultivation of algae cells, and pass 2% CO into the culture medium 2 Mix air to adjust the pH of the culture medium to between 7 and 8. In the process of algae cell cultivation, monitor the ion concentration of bicarbonate in the culture medium, and adjust the CO in the mixed air according to the concen...

Embodiment 2

[0055] Control group 2, culture group 2 and culture group 3 all inoculated a strain of self-isolated algae in the logarithmic growth phase in a 5cm optical path tube reactor, the light was 150μmol / ㎡ / s, 8h: 16h light-dark cycle , the temperature is room temperature, and the initial culture solution is the improved BG11 culture solution suitable for the growth induction of the algae. The formula of the improved BG11 culture solution is shown in Attached Table 1.

[0056] Both culture group 2 and culture group 3 added 1.26g / L NaHCO to the initial culture medium 3 , then the bicarbonate ion concentration in the culture solution is about 15mmol / L. Breeding Group 2 and Breeding Group 3 both add algae species to the prepared culture medium and start culturing algae cells. Breeding Group 2 adds acetic acid to the culture medium to adjust the pH value of the culture medium to between 7-8 ; The culture group three added hydrochloric acid to the culture medium to adjust the pH value of ...

Embodiment 3

[0061] Control group 3, culture group 4 and culture group 5 all inoculated a strain of self-isolated algae in the logarithmic growth phase in a 0.2㎡ small track pool, with an optical path of 10cm and an illumination of 700μmol / ㎡ / s for 16h: 8h light and dark cycle, the temperature is room temperature, the initial culture medium is the improved BG11 medium suitable for microalgae growth induction, and the formula of the improved BG11 culture medium is shown in Attached Table 1.

[0062] Breeding group four is to add 0.84g / L NaHCO to the initial culture medium 3 , then the bicarbonate ion concentration in the culture medium is about 10mmol / L. Breeding group five is to add 1.68g / L NaHCO in the initial culture medium 3 , then the bicarbonate ion concentration in the culture solution is about 20mmol / L. Insert the algae species into the prepared culture medium and start the culture of the algae cells. Both the culture group 4 and the culture group 5 are injected with 10% CO2 into t...

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Abstract

The invention provides a microalgae culture method. The method comprises steps as follows: preparation: a culture solution for microalgae growth is prepared, wherein the culture solution contains bicarbonate radical ions; culture: the culture solution is inoculated with algae, the pH value of the culture solution is adjusted to a preset pH value, the concentration of the bicarbonate radical ions in the culture solution is adjusted to reach first preset concentration of 10-30 mmol / L, and algae cell culture is performed; oil production: when the algae cell yield is reduced, the concentration ofthe bicarbonate radical ions in the culture solution is adjusted to reach second preset concentration of 0-20 mmol / L, and oil production is performed; harvesting: when the oil yield is reduced, oily algae cells are harvested. Carbon source substrates required by growth of the algae cells can be guaranteed, so that the algae cells are increased quickly, the yield of microalgae biomass is increased,the algae cells can accumulate more oil under the action of sufficient carbon source substrates, the content and yield of microalgae oil are increased, and carbon dioxide in the atmosphere is fully utilized.

Description

technical field [0001] The invention relates to the technical field of microalgae, in particular to a method for cultivating microalgae. Background technique [0002] Microalgae are a type of photoautotrophic organisms that grow in water and can absorb CO under the drive of light energy. 2 , Convert light energy into chemical substances stored in cells, and release oxygen at the same time. The cell metabolism of microalgae produces products such as polysaccharides, proteins, oils, and pigments. These metabolites have good development prospects in the fields of food, medicine, and liquid fuels. [0003] With the rapid development of the global economy, the demand for energy is increasing day by day, making the main energy source, such as traditional fossil fuels such as oil and coal, decreasing day by day. Moreover, the combustion of traditional fossil fuels releases a large amount of CO 2 , leading to global warming and seawater acidification, resulting in huge environmen...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 尹顺吉蔡禹希赵露华白雪梅吴洪
Owner ENN SCI & TECH DEV
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