Method for modifying starch according to enzymatic degradation process

A technology of enzymatic degradation and starch, applied in the field of starch modification, can solve problems such as difficult esterification, and achieve the effect of changing adsorption properties, good stability, and improving degree of substitution

Inactive Publication Date: 2018-01-16
SHANDONG SHENZHOU XIANGYU TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since porous starch is formed by amylase hydrolyzing the amorphous region of starch, the esterification reaction also mainly occurs in the amorphous region of starch, which makes porous starch more difficult to esterify than ordinary starch

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In an embodiment of the present invention, a method for enzymatically degrading modified starch comprises the following steps:

[0023] 1) Mix the starch with 4 times the mass of water, add epichlorohydrin and phosphate buffer solution, then heat and stir at 40°C for 0.5h, then add sodium trimetaphosphate with a mass of 3% of the starch, and mix well , react at 40°C for 0.5h, filter with suction, wash, and dry at 55°C to obtain mixture A; the mass ratio of starch, epichlorohydrin and phosphate buffer solution is 5:1:2.

[0024] 2) Disperse the mixture A in a certain amount of deionized water, then add α-amylase and glucoamylase, react at 35°C for 10h under stirring conditions, sonicate for 0.5h, deenzyme, filter, wash, and dry at 55°C. Pulverize to prepare mixture B; the mass ratio of α-amylase and glucoamylase is 1:5. The addition amount of α-amylase and glucoamylase is 1% of the mass of mixture A. Ultrasonic power is 500W.

[0025] 3) Disperse the mixture B in a ce...

Embodiment 2

[0027] In an embodiment of the present invention, a method for enzymatically degrading modified starch comprises the following steps:

[0028] 1) Mix the starch with 10 times the mass of water, add epichlorohydrin and phosphate buffer solution, then heat and stir at 65°C for 4 hours, then add sodium trimetaphosphate with 30% of the mass of starch, and mix well, After reacting at 60°C for 3 hours, filter with suction, wash, and dry at 70°C to obtain mixture A; the mass ratio of starch, epichlorohydrin and phosphate buffer solution is 20:1:10.

[0029] 2) Disperse the mixture A in a certain amount of deionized water, then add α-amylase and glucoamylase, react at 65°C for 30h under stirring conditions, sonicate for 2h, deenzyme, filter, wash, dry at 70°C, and pulverize , to prepare mixture B; the mass ratio of α-amylase and glucoamylase is 1:10. The addition amount of α-amylase and glucoamylase is 15% of the quality of mixture A. Ultrasonic power is 500W.

[0030] 3) Disperse ...

Embodiment 3

[0032] In an embodiment of the present invention, a method for enzymatically degrading modified starch comprises the following steps:

[0033] 1) Mix the starch with 7 times the mass of water, add epichlorohydrin and phosphate buffer solution, then heat and stir at 50°C for 2 hours, then add sodium trimetaphosphate with 10% of the mass of starch, and mix well, After reacting at 50°C for 2h, filter with suction, wash, and dry at 60°C to obtain mixture A; the mass ratio of starch, epichlorohydrin and phosphate buffer solution is 12:1:6.

[0034] 2) Disperse the mixture A in a certain amount of deionized water, then add α-amylase and glucoamylase, react at 45°C for 20h under stirring conditions, sonicate for 1h, deenzyme, filter, wash, dry at 60°C, and pulverize , to prepare mixture B; the mass ratio of α-amylase and glucoamylase is 1:8. The addition amount of α-amylase and glucoamylase is 5% of the mass of mixture A. Ultrasonic power is 300W.

[0035] 3) Disperse the mixture ...

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PUM

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Abstract

The invention discloses a method for modifying starch according to an enzymatic degradation process. The method comprises the following steps: mixing the starch with water, adding epoxy chloropropaneand a phosphate buffer solution, then performing heating stirring at temperature of 40 to 65 DEG C for treatment for 0.5 to 4 hours, then adding sodium trimetaphosphate, performing uniform mixing, generating reaction at temperature of 40 to 60 DEG C for 0.5 to 3 hours, and performing filtration, washing and drying, thereby obtaining a mixture A; dispersing the mixture A into deionized water, adding alpha-amylase and saccharifying enzyme, performing a reaction at 35 to 65 DEG C under a stirring condition for 10 to 30 hours, performing ultrasonic treatment for 0.5 to 2 hours, and performing enzyme removal, filtration, washing, drying and smashing, thereby obtaining a mixture B; dispersing the mixture B into the deionized water, adding acetic acid for stirring treatment reaction for 5 to 15 hours, neutralizing the obtained solution with a sodium hydroxide solution, and performing filtration, washing and drying, thereby obtaining the starch. The method can effectively increase the substitution degree, keep honeycomb holes of porous starch intact and change the adsorption quality of the porous starch, and is high in product stability; the process is simple, and is easy to operate.

Description

technical field [0001] The invention relates to the technical field of starch modification, in particular to a method for modifying starch by an enzyme degradation process. Background technique [0002] Starch is the aggregation of glucose molecules, which is the most common storage form of carbohydrates in cells. Starch is a nutrient stored in plants, stored in seeds and tubers, and the content of starch in all kinds of plants is relatively high. Starch can be regarded as a high polymer of glucose. In addition to food, starch is used industrially to make dextrin, maltose, glucose, alcohol, etc. It is also used to prepare printing paste, sizing of textiles, gluing of paper, and pressing of pharmaceutical tablets. It can be extracted from starchy substances such as corn, sweet potatoes, wild acorns and kudzu root. [0003] Starch is a raw material whose molecular structure can be degraded and has thermoplastic properties. However, due to its poor water solubility, its chem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04
Inventor 任思凯李超周忠伟袁美宏任思龙
Owner SHANDONG SHENZHOU XIANGYU TECH GRP
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