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An aptamer-regulated activity-absorption spectrum of nano-silica for the determination of hg 2+ Methods

A nano-silica and absorption spectrum technology, applied in the field of analytical chemistry, to achieve the effect of simple method, high sensitivity and stable system

Inactive Publication Date: 2020-03-31
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As a new nanomaterial, nano-silica is more and more widely used. The application of aptamers to regulate the catalysis of nano-silica non-metallic nano-enzymes and the application of surface plasmon resonance absorption spectroscopy to the quantitative determination of Hg 2+ Analytical methods have not yet been reported

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  • An aptamer-regulated activity-absorption spectrum of nano-silica for the determination of hg <sup>2+</sup> Methods

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Embodiment

[0021] A method of regulating the activity-absorption spectrum of nano-silica to measure Hg by aptamer 2+ method, including the following steps:

[0022] (1) Prepare a known concentration of Hg 2+ Standard solution system: Add 10 μL, 50 μL, 100 μL, 150 μL, 200 μL of 100 nmol / L Hg into 7 graduated test tubes 2+ Standard solution, then add 100 μL of 66ng / mL mercury aptamer with the sequence 5'-TTTCTTCTTTTCTCCCCCTTGTTTGTTGTTT-3' and 12 μL of 100 μg / mL nano-silica to each graduated test tube, mix well, and let stand for 10 minutes; Then add 150 μL of 0.5 moL / L glucose, 150 μL of 0.01 moL / L HCl and 100 μL of 84 μmoL / L HAuCl to each test tube in sequence 4 , mix well, dilute to 1.5 mL with twice distilled water, react in a water bath at 75°C for 20 minutes, take out the test tube, cool with ice water to terminate the reaction, and dilute to 2.0 mL with twice distilled water;

[0023] (2) Prepare the blank control solution system: use the method of step (1) without adding Hg 2+ S...

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Abstract

The invention discloses a Hg2+ measuring method through using an aptamer-regulated nanometer silica activity and absorption spectrum. The method is characterized by comprising: (1) preparing a Hg2+ standard solution system with a known concentration, and measuring the absorbance value A of the system; (2) preparing a blank contrast solution system, and measuring the absorbance value A<0> of the system; (3) calculating [delta]A=A-A<0>; (4) making a working curve of the relation between [dela]A and the Hg2+ concentration; (5) preparing a to-be-tested sample solution, measuring the absorbance value A<sample>, and calculating [dela]A<sample>= A<sample>-A<0>; and (6) calculating the Hg2+ content of the sample solution according to the working curve obtained in the step (4). Compared with a method in the prior art, the method doesn't require a complex process of building an aptamer nanoprobe, and is simple and fast. Non-metal nano-enzyme is high in sensitivity, so that the system is stable.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for regulating the activity of nano-silica with an aptamer-absorption spectrum for measuring Hg 2+ Methods. Background technique [0002] Aptamer is a short oligonucleotide sequence obtained by in vitro screening, which can bind to a variety of target substances with high specificity, high affinity and high selectivity. When the nucleic acid aptamer specifically binds to the target substance, The configuration of the nucleic acid aptamer itself will change, and its detection signal will change accordingly to realize the detection of the target. Mercury is a highly toxic heavy metal. Mercury can easily accumulate in humans and animals, especially fish, through the food chain. Even at very low concentrations, mercury will cause great harm to humans and the environment. long-term toxic effects. Therefore, it is of great significance to study the determination method o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 李重宁梁爱惠蒋治良
Owner GUANGXI NORMAL UNIV