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Method for preparation of vasicine and vitexin from adhatoda vasica

A technology for the preparation of orchidine and vitexin, which can solve the problems of poor extraction effect and difficult to meet the extraction requirements, so as to improve the dissolution rate and output, and reduce environmental waste possible, the effect of reducing production costs

Inactive Publication Date: 2018-01-19
长沙爱扬医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the reports of extracting orchidine and vitexin from plants at the same time are very rare, and the existing methods for extracting orchidine and vitexin are used to extract orchidine and vitexin from Duckbill flower. The extraction effect is poor , it is difficult to meet the extraction requirements

Method used

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  • Method for preparation of vasicine and vitexin from adhatoda vasica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Crush the dried duckbill flower stem and leaf raw materials to 10-20 mesh, weigh 100Kg, add 10L of Aspergillus crassasporium culture solution, and carry out aerobic solid fermentation at 28-30°C for 3-5 days. Add 800L of 80% ethanol solution to Duckbill flower after fermentation, ultrasonically extract 3 times at room temperature, 30 minutes each time, filter twice with three layers of gauze, filter once with ceramic membrane, combine the filtrate, concentrate the filtrate under reduced pressure and vacuum dry to remove ethanol , to obtain 6.2kg of dry matter I. The dried product I was leached with chloroform for 4-5 hours, filtered with a ceramic membrane, and the filter residue was vacuum-dried to obtain 1.41 kg of dried product II (flavone); the filtrate was concentrated under reduced pressure and vacuum-dried to remove chloroform, and dried to obtain 3.72 kg of dried product III. Dissolve the dried product II in water, filter with ceramic membrane, purify the filtra...

Embodiment 2

[0037] Crush the dried duckbill flower stem and leaf raw materials to 10-20 mesh, weigh 200Kg, add 15L Aspergillus crassasporium culture solution, and carry out aerobic solid fermentation at 28-30°C for 3-5 days. Add 1800L of 80% ethanol solution to Duckbill flower after fermentation, ultrasonically extract 3 times at room temperature, 30 minutes each time, filter twice with three layers of gauze, filter once with ceramic membrane, combine the filtrate, concentrate the filtrate under reduced pressure and vacuum dry to remove ethanol , to obtain 11.4kg of dry matter I. The dried product I was leached with chloroform for 4-5 hours, filtered with a ceramic membrane, and the filter residue was vacuum-dried to obtain 2.78 kg of dried product II (flavone); the filtrate was concentrated under reduced pressure and vacuum-dried to remove chloroform, and dried to obtain 7.41 kg of dried product III. Dissolve the dried product II in water, filter with ceramic membrane, purify the filtrat...

Embodiment 3

[0040] Crush the dried duckbill flower stem and leaf raw materials to 10-20 mesh, weigh 5Kg, add 0.5L Aspergillus crassasporium culture solution, and carry out aerobic solid fermentation at 28-30°C for 3-5 days. Add 40L of 80% ethanol solution to Duckbill flower after fermentation, ultrasonically extract 3 times at room temperature, 30 minutes each time, filter twice with three layers of gauze, filter once with ceramic membrane, combine the filtrate, concentrate the filtrate under reduced pressure and vacuum dry to remove ethanol , to obtain 295g of dry matter I. The dried product I was leached with chloroform for 4-5 hours, filtered with a ceramic membrane, and the filter residue was vacuum-dried to obtain 70.2 g of dried product II (flavone); the filtrate was concentrated under reduced pressure and vacuum-dried to remove chloroform, and dried to obtain 192 g of dried product III. Dissolve the dried product II in water, filter with ceramic membrane, purify the filtrate with D...

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Abstract

The invention provides a method for synchronous extraction of vasicine and vitexin from adhatoda vasica. The method includes: subjecting dried adhatoda vasica stems and leaves to microbial fermentation, then using ethanol and chloroform solutions for extraction, and carrying out fractionation, filtering, pressure reduced concentration, adsorption column chromatography, ion exchange chromatography,decolorization and crystallization, thus obtaining vitexin and vasicine pure products with purity of more than 95%. The method employs aspergillus for fermentation treatment, effectively reduces theinfluence of starch and fiber in adhatoda vasica on extraction of active substances, and improves the dissolution rate and yield of vasicine and vitexin. Ethanol and chloroform are employed for synchronous extraction of vitexin and vasicine, and a variety of active ingredients can be acquired rapidly.

Description

technical field [0001] The invention belongs to the field of natural product processing, and relates to a method for preparing orthocyanine and vitexin from duckbill flowers. Background technique [0002] Duck-billed flower is also called big resurgence, big rebuff bone, big joint bone, etc. It is an evergreen shrub of the family Acanthaceae Duck-billed flower. It is mainly distributed in southeastern Asia, in Guangdong, Guangxi, Hainan, Macau, Hong Kong, Yunnan and other places in my country. Productive, or cultivated, or wild. The main characteristics of the plant are: large shrub, up to 1-3 meters; branches cylindrical, gray, with lenticels, young branches densely covered with off-white puberulent. Leaves papery, oblong-lanceolate to lanceolate, or ovate or elliptic-ovate, 15-20 cm long, 4.5-7.5 cm wide, apex acuminate, sometimes slightly caudate, base broadly cuneate, Margin entire, subglabrous above, puberulent abaxially; midvein grooved above, lateral veins about 12 o...

Claims

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Application Information

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IPC IPC(8): C07D487/04C07D407/04
Inventor 罗婵
Owner 长沙爱扬医药科技有限公司
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