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Preparation method for chitohexaose hydrochloride to octasaccharide chrome complex

A technology of chromium complex and chitohexaose is applied in the field of preparation of chitosan oligosaccharide complex, can solve the problems of low degree of polymerization, high price, unfavorable industrialized production, etc. Effect

Inactive Publication Date: 2018-01-19
贾晗 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The chitosan oligosaccharide obtained by degrading chitosan in the prior art has a wide molecular weight range, and the degree of polymerization (Degree of Polymerization, DP) is usually higher than 20, and the specific enzyme for degrading chitosan in the prior art-- -The price of chitosanase is relatively expensive, which is not conducive to industrial production. Therefore, the functional application of chitosan oligosaccharides in the fields of food, medicine, and cosmetics is restricted. At present, it is urgent to obtain chitin degradation methods for chitosan oligosaccharides. Chitohexaose to octaose chromium complexes with low degree and good binding effect on the binding target of glucose tolerance factor

Method used

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  • Preparation method for chitohexaose hydrochloride to octasaccharide chrome complex
  • Preparation method for chitohexaose hydrochloride to octasaccharide chrome complex
  • Preparation method for chitohexaose hydrochloride to octasaccharide chrome complex

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Effect test

Embodiment 1

[0042] A preparation method of chitosexaose to octaose chromium complex comprises the following steps:

[0043] Step 1: Chitosan with a deacetylation degree of more than 90% is formulated with 1% acetic acid solution into 5% chitosan acetic acid solution,

[0044] Step 2 Under the conditions of pH 5.5 and temperature 55°C, add a compound enzyme preparation consisting of cellulase and papain at a concentration of 0.05% with a mass ratio of 1:1, and perform enzymatic hydrolysis for 5 hours.

[0045] Step 3. Heat up to 70°C and keep the enzyme inactivated for 45 minutes. After cooling to room temperature, filter for the first time: filter with qualitative filter paper, adjust the pH of the filtrate to 7.5, and heat to 50°C to obtain the primary filtrate. Separation and purification: the molecular weight cut-off MWCO is 3KDa The ultrafiltration membrane was separated and purified at a circulating temperature of 50°C to obtain the permeate, and the third filtration: the permeate wa...

Embodiment 2

[0051] A preparation method of chitosexaose to octaose chromium complex comprises the following steps:

[0052] Step 1: Chitosan with a deacetylation degree of more than 70% is formulated with 1% acetic acid solution into 5% chitosan acetic acid solution,

[0053] Step 2 Under the conditions of pH 5.5 and temperature 55°C, add a compound enzyme preparation composed of cellulase and papain at a concentration of 0.05% in a mass ratio of 1:1, and perform enzymatic hydrolysis for 6 hours.

[0054] Step 3: heat up to 80°C for 30 minutes to inactivate the enzyme, cool to room temperature and then filter for the first time: filter with qualitative filter paper, adjust the pH of the filtrate to 8, heat to 50°C to obtain the primary filtrate, separate and purify: take the molecular weight cut-off as 1.5KDa The ultrafiltration membrane was separated and purified at a cycle temperature of 50°C to obtain the permeate, and the third filtration: the permeate was a negatively charged nanofil...

Embodiment 3

[0059] A preparation method of chitosexaose to octaose chromium complex, is characterized in that, comprises the following steps:

[0060] Step 1: the chitosan with a deacetylation degree of more than 55% is prepared with a concentration of 2% acetic acid solution into a 5% chitosan acetic acid solution;

[0061] Step 2. Under the conditions of pH 5.5 and temperature 55°C, add a compound enzyme preparation of cellulase, papain and lipase with a concentration of 0.05% in a mass ratio of 5:4:1 to carry out enzymolysis reaction. Solution reaction time 7h;

[0062] Step 3: Raise the temperature to inactivate the enzyme reaction, cool to room temperature, filter, separate and purify the enzymolysis liquid, filter once, filter with qualitative filter paper, adjust the pH of the filtrate to 8, heat to 50°C to obtain the primary filtrate, separate and purify the primary filtrate The filtrate was separated and purified with an ultrafiltration membrane with a molecular weight cut-off o...

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Abstract

The invention relates to a preparation method for chitohexaose hydrochloride to octasaccharide chrome complex. The method comprises the following steps: 1) preparing the chitosan at degree of deacetylation above 55-90% and acetum into chitosan acetum; 2) adopting a cellulase and papain compound enzyme preparation for performing enzymatic hydrolysis; 3) raising temperature and inactivating enzyme reaction, cooling to room temperature, filtering, separating and purifying enzymatic hydrolysate; 4) adding a CrCL3 solution for complexing reaction with zymolyte; and 5) performing vacuum concentration and precipitating, thereby generating the chitohexaose hydrochloride to octasaccharide chrome complex. According to the preparation method provided by the invention, a complex enzyme preparation isadopted for performing enzymolysis on macromolecular chitosan, the molecular weight is controlled at 1300Da, the product reacts with Cr<3+> so as to form the chrome complex, the binding activity of glucose tolerance factor is promoted, the decomposing cost is lowered and the product safety is increased.

Description

technical field [0001] The invention relates to a preparation method of a chitosan oligosaccharide complex, in particular to a preparation method of a chitosan hexaose to octasaccharide chromium complex. Background technique [0002] Diabetes (diabetes) is an endocrine and metabolic disease related to abnormal insulin production and action and characterized by hyperglycemia, mainly including type 1 and type 2 diabetes. According to the latest statistics, the number of diabetic patients in my country has reached 114 million, accounting for one-third of the total number of diabetic patients in the world, becoming the world's largest diabetes country. According to the survey report, type II diabetes is the main type of diabetes population in my country, accounting for more than 90% of the total number of diabetic patients. Type II diabetes, which is mainly based on the relative lack of insulin and insulin resistance, will eventually lead to hyperglycemia, and long-term hypergl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 贾晗邢燕青
Owner 贾晗
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