Quantitative detection method based on full-disc imaging system of Blu-ray disc drive

Abstract

The invention provides a quantitative detection method based on a blue light optical disc drive full compact disc imaging system. According to the quantitative detection method, a biological detectionarray is prepared on a standard blue light compact disc; the compact disc is read by using a standard blue light optical disc drive, and a full compact disc image file is formed by dimension conversion, feature alignment, mean scaling and coordinate system transformation of the full compact disc imaging system of the standard blue light optical disc drive performed on the acquired light intensitysignals; and the generated image file is analyzed so that the quantitative detection result of the target biomolecules can be obtained. The quantitative detection method has the advantages of being multi-flux, high in sensitivity and feasible in onsite rapid detection so as to be suitable for the important field of food safety, water quality detection and medical diagnosis.

Description

technical field [0001] The invention relates to a biomolecular imaging analysis and quantitative detection method, in particular to a quantitative detection method of a standard Blu-ray disc imaging system based on LabVIEW, MATLAB and C++ hybrid programming. Background technique [0002] At the end of 1970, optical disc technology came out. Because the surface of the optical disc is very smooth and has good optical properties, the treated optical disc surface can specifically bind protein and DNA probe molecules, and then a detection array structure can be prepared on the optical disc surface. When the optical drive system laser beam When the optical disc is scanned over the optical disc detection array, the path of the laser beam is disturbed, and the intensity of the beam measured by the photodiode is correspondingly reduced. Wherein, the amplitude change of the optical signal received by the photodetector in the optical head can be used for quantitative analysis. [0003...

AI Technical Summary

Problems solved by technology

[0006] Different forms of modification of optical drive make it a new platform technology for molecular recognition and detection. However, due to the particularity of the modification, these modifications will affect the ability of the optical drive to read the optical disc or even destroy the optical drive, and some require the production of special optical discs , these have greatly reduced the advantages of molecular recognition and detection technology based on CD / CD-ROM

Images