A kind of preparation method of biological pesticide preparation for preventing and controlling red fire ants

A biopesticide and red fire ant technology, applied in botany equipment and methods, biocide, animal repellent, etc., can solve the problems of slow speed, affecting the insecticidal activity of Beauveria bassiana, and low spore germination rate, etc. And convenient transportation, increase insecticidal effect, and improve the effect of germination environment

Active Publication Date: 2020-08-04
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when Beauveria bassiana is used in the field, due to the limitation of temperature, humidity, ultraviolet light and other environmental conditions, the germination rate of spores is low, which affects the insecticidal activity of Beauveria bassiana. Therefore, the use of pathogenic microorganisms Beauveria bassiana alone The rate of control of RIFA by B. anisopliae is relatively slow

Method used

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  • A kind of preparation method of biological pesticide preparation for preventing and controlling red fire ants
  • A kind of preparation method of biological pesticide preparation for preventing and controlling red fire ants
  • A kind of preparation method of biological pesticide preparation for preventing and controlling red fire ants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Indoor virulence determination experiment of red imported fire ant worker ants with Beauveria bassiana and sodium chloride

[0026] In the laboratory toxicity test, weigh 5 g of Beauveria bassiana powder and dissolve it in 1L of water, mix well, and obtain a Beauveria bassiana solution. The Beauveria bassiana powder used contains 20 billion Beauveria bassiana spores / g. Measure 9 parts of 100ml of Beauveria bassiana solution from 1L of Beauveria bassiana solution, and then add NaCl to them respectively, so that the concentration of NaCl in 9 parts of Beauveria bassiana solution is: 0mmol / L, 25mmol / L, 75mmol / L, 100mmol / L, 125mmol / L, 150mmol / L, 200mmol / L, 250mmol / L, 300mmol / L, sequentially obtain Beauveria bassiana·sodium chloride aqueous dispersion solution, in which the spore concentration of Beauveria bassiana is 1× 10 8 Pcs / mL -1 .

[0027] The virulence of Beauveria bassiana solution, Beauveria bassiana·sodium chloride aqueous dispersion solution, sodium chlo...

Embodiment 2

[0030] Example 2: Indoor Toxicity Test of Beauveria bassiana·Sodium Chloride Water Dispersant on Red Imported Fire Ant Worker Ant

[0031] Such as image 3 with Figure 4 As shown, the red imported fire ant control drug preparation mixer of this embodiment includes a liquid container 8, a solid container 4, a rubber gasket 3, and a cover 2. The solid container 4 is used for the upper end and the lower end. Aluminum foil composite film sealed container 5, the solid container is provided with a snap ring 9 on the edge of the opening, a rubber gasket 6 is placed on the bottle mouth 7 of the liquid container, and the solid container is inserted through the rubber gasket 6 In the mouth 7 of the liquid container, the snap ring 9 is pressed on the rubber gasket 6, the rubber gasket 3 is pressed on the snap ring 9, and the cover 2 connects the rubber gasket 3, the snap ring 9, and the rubber gasket. The gasket 6 is sleeved and fixed on the bottle mouth 7 of the liquid container, and a f...

Embodiment 3

[0041] In the laboratory toxicity determination, weigh 1 g of Beauveria bassiana powder and 7.5 g of sodium chloride, dissolve in 1L of water, mix well, and obtain a Beauveria bassiana solution. The Beauveria bassiana powder used contains 20 billion Beauveria bassiana spores / g.

[0042] The indoor toxicity test was carried out according to the method of Example 1, and the results showed that the Beauveria bassiana solution of this example has a better killing effect on red fire ants.

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Abstract

The invention discloses a preparation method of a biological pesticide preparation for preventing and controlling red fire ants a. preparing a medicine for preventing and controlling red fire ants containing pathogenic microorganisms, and the medicine for preventing and controlling red fire ants containing pathogenic microorganisms contains Beauveria bassiana and chlorinated Sodium, the number of Beauveria bassiana spores and the gram ratio of sodium chloride are: (1~8)×20 billion: 4.0~7.5g; b, put the medicament of preventing and treating red fire ants containing pathogenic microorganisms in a solid container In the liquid container, there is a corresponding amount of solution in the liquid container, and then the solid container is inserted into the liquid container, the snap ring is stuck on the bottle mouth of the liquid container, and then the rubber gasket is pressed against the snap ring. On the ring, the cover covers the rubber gasket and snap ring and fixes it on the bottle mouth of the liquid holder. The biopesticide preparation for preventing and controlling red fire ants of the present invention has long storage time, convenient use and transportation, saves time and effort, and can be used immediately after preparation. After use, the germination environment of the drug (spore) is improved, the insecticidal effect is increased, and finally the The purpose of controlling red fire ants in the field.

Description

Technical field: [0001] The invention belongs to the field of red fire ant prevention and control, and specifically relates to a preparation method of a biological pesticide preparation for preventing and controlling red fire ants. Background technique: [0002] Beauveria bassiana: Beauveria bassiana is a kind of entomogenous fungus of ascomycete. Beauveria bassiana is non-toxic, tasteless, non-environmental pollution, and has continuous infectivity to pests. Once infected, pests can continuously infect and spread. No pesticide residues, even if the product is harvested immediately after applying Beauveria bassiana, it will not cause any pesticide residues; no resistance, Beauveria bassiana is infected and killed by contact with the pest's body wall under natural conditions, and the pests will not produce any resistance to it. It is more and more effective after years of use. Regrowth, Beauveria bassiana new biological pesticide contains live fungi and spores. After being applied...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/30A01N59/08A01P7/04
CPCA01N63/30A01N25/14A01N25/22A01N25/04A01N59/08
Inventor 李军韩诗畴叶静文押玉柯吕欣
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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