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Extraction method of basil seed crude polysaccharides

An extraction method and technology of crude polysaccharides, which are applied in the field of extracting crude polysaccharides from orchids, can solve the problems of difficult extraction and analysis of plant polysaccharides, complex polysaccharide structures, etc., and achieve the effects of retaining biological activity, short extraction time, and improving purity

Inactive Publication Date: 2018-01-26
丘少荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polysaccharides have complex structures and various types, and they are often combined with lipids and proteins to form polysaccharide complexes, which brings many difficulties to the extraction and analysis of plant polysaccharides.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for extracting the crude polysaccharide of Lanxiangzi, including the following steps:

[0032] (1) Wash the Lanxiangzi and dry it, add small molecule water according to the ratio of 1:1 to the material-to-liquid ratio, beat the slurry in a homogenizer, put the slurry in an ultrasonic water bath, and in a water bath at 150W and 70℃ for 1h , Then add an appropriate amount of small molecule water for ultrasonic extraction, and the total amount of small molecule water added twice to the material-to-liquid ratio of Lanxiangzi is 1:50;

[0033] (2) Centrifuge at 4000r / min for 6min to separate the supernatant, put the precipitate to room temperature and add small molecule water to the material-liquid ratio of 1:50, then add 2% composite enzyme solution, adjust the pH with potassium dihydrogen phosphate To 5, carry out enzymatic hydrolysis at 40°C for 50 minutes, where the complex enzyme solution is pectinase: papain: xylanase = 5:1:2, then heat to 80°C for sterilization, c...

Embodiment 2

[0036] A method for extracting the crude polysaccharide of Lanxiangzi, including the following steps:

[0037] (1) Wash the Lanxiangzi and dry it, add small molecular water according to the material-to-liquid ratio of 1:1.5, beat the slurry in a homogenizer, put the slurry in an ultrasonic water bath, and place it in a water bath at 200W and 75℃ for 1.5 h. Then add an appropriate amount of small molecule water for ultrasonic extraction. The total amount of the two added small molecule water and the material-to-liquid ratio of Lanxiangzi is 1:52;

[0038] (2) Centrifuge at 4300r / min for 9min to separate the supernatant, put the precipitate at room temperature, add small molecule water to the material-liquid ratio of 1:52, then add 2.5% composite enzyme solution, adjust the pH with potassium dihydrogen phosphate To 6, carry out enzymatic hydrolysis at 42°C for 52 minutes, where the compound enzyme solution is pectinase: papain: xylanase = 5:1:2, then heat to 85°C for sterilization, c...

Embodiment 3

[0041] A method for extracting the crude polysaccharide of Lanxiangzi, including the following steps:

[0042] (1) Wash the Lanxiangzi and dry it, add small-molecule water according to the ratio of 1:2 to the material-to-liquid ratio, beat the slurry in a homogenizer, put the slurry in an ultrasonic water bath, and bath in a water bath at 250W and 80℃ for 2h , And then add an appropriate amount of small molecule water for ultrasonic extraction. The total amount of small molecule water added twice and the material-to-liquid ratio of Lanxiangzi is 1:55;

[0043] (2) Centrifuge at 4500r / min for 12 minutes to separate the supernatant. Put the precipitate to room temperature and add small molecule water to a material-to-liquid ratio of 1:55, then add 3% composite enzyme solution, adjust the pH with potassium dihydrogen phosphate To 6, carry out enzymatic hydrolysis at 45°C for 55 minutes, where the compound enzyme solution is pectinase: papain: xylanase = 5:1:2, then heat to 90°C for st...

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PUM

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Abstract

The invention relates to the field of plant extraction, in particular to an extraction method of basil seed crude polysaccharides. According to the extraction method, basil seeds are beaten into pulp,by means of combination of high-temperature ultrasonic extraction and enzyme extraction, plant cell walls can be damaged, release of polysaccharides in cells is facilitated, and dissolution rate of the polysaccharides is increased. By means of combination of high-temperature ultrasonic extraction and enzyme extraction, the plant cell walls can be damaged, release of the polysaccharides in the cells is facilitated, and the dissolution rate of the polysaccharides is increased.

Description

Technical field [0001] The invention relates to the field of plant extraction, in particular to a method for extracting crude polysaccharides from Lanxiangzi. Background technique [0002] Lanxiangzi, also known as basil, is the fruit of basil, a plant of the Lamiaceae. The dried nutlets are ovoid in shape, about 2 mm long, with traces of the stalk at the base; the surface is gray-brown to black, with a slight luster, and fine dots can be seen under an enlarged lens. The texture is hard, the cross section is triangular, the cotyledons are thick, milky white, and rich in oil. It is weak in breath, light in taste, and smooth in the mouth; after soaking in water and swelling, there is a layer of white mucus on the surface. The white thing wrapped around the seeds is a substance called "mucopolysaccharide". The fruit mucilage contains 9.2% pentose, 70.3% hexose, 7.3% uronic acid, 25.4% free lipid, and 10.3% combined lipid. The mucopolysaccharide is hydrolyzed to produce D-galactu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 丘少荣
Owner 丘少荣
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