Method for preparing flocculating agents by using compound strains

A technology of flocculant and compound bacteria, which is applied in the field of water treatment, can solve the problems of low flocculation activity, achieve the effects of promoting sedimentation, reducing the use of ethanol, and stabilizing performance

Active Publication Date: 2018-01-30
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the problem of low flocculation activity in the preparation of exopolysaccharide flocculants by microorganisms at the present stage, the purpose of the present invention is to provide a method for preparing flocculants using a compound bacterial agent. The compound bacterial agent is Halomonas marine strain Halomonassp.GHF1 Psychrobacter sp.GHF2 and Psychrobacter sp.GHF2, the two strains are isolated and purified from the sludge spit out by clam philippines, and the two strains are used in combination to strengthen each other during the fermentation process, and the yield of exopolysaccharide produced High, the flocculation activity of exopolysaccharide is strong, the flocculation ability of the obtained flocculant is strong, and the performance is stable

Method used

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  • Method for preparing flocculating agents by using compound strains
  • Method for preparing flocculating agents by using compound strains
  • Method for preparing flocculating agents by using compound strains

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Embodiment 1

[0041] A method for preparing a flocculant by using a compound bacterial agent, comprising the following steps:

[0042] (1) Inoculate the strains of Halomonas sp.GHF11 and Psychrobactersp.GHF2 on solid medium and culture them for 36 hours. The culture temperature of Halomonas sp.GHF11 is 25°C The culture temperature of Psychrobacter sp.GHF2 was 18°C, and sterile water was added to make GHF11 strain liquid and GHF2 strain liquid respectively, and the volume ratio of sterile water to solid medium was 1.0:1;

[0043] (2) According to the mixing volume ratio of GHF11 strain liquid and GHF2 strain liquid, the two strain liquids were mixed to obtain the mixed strain liquid, and then inoculated into the liquid culture medium, the inoculation concentration of the mixed strain liquid was 1.5mL mixed strain solution / 100mL liquid culture medium, fermented and cultured at 23°C for 3 days, and the shaking table rotated at 100r / min, to make a fermentation broth;

[0044] (3) Add flocculat...

Embodiment 2

[0053] A method for preparing a flocculant by using a compound bacterial agent, comprising the following steps:

[0054] (1) The strains of Halomonas sp.GHF11 and Psychrobacter sp.GHF2 were inoculated on solid medium for 42 hours, and the culture temperature of Halomonas sp.GHF11 was 28°C , the culture temperature of Psychrobacter sp.GHF2 was 20°C, and sterile water was added to make GHF11 strain liquid and GHF2 strain liquid respectively, and the volume ratio of sterile water to solid medium was 1.25:1;

[0055] (2) According to the mixed volume ratio of GHF11 strain liquid and GHF2 strain liquid as 1:1, mix the two strain liquids to obtain the mixed bacterial liquid, and then inoculate it into the liquid culture medium, the inoculation concentration of the mixed bacterial liquid is 2.0mL mixed bacteria solution / 100mL liquid culture medium, fermented and cultured at 25°C for 4 days, and the shaking table rotated at 140r / min, to make a fermentation broth;

[0056] (3) Add flo...

Embodiment 3

[0065] A method for preparing a flocculant by using a compound bacterial agent, comprising the following steps:

[0066] (1) Inoculate the strains of Halomonas sp.GHF11 and Psychrobactersp.GHF2 on solid medium and culture them for 48 hours. The culture temperature of Halomonas sp.GHF11 is 30°C , the culture temperature of Psychrobacter sp.GHF2 was 23°C, and sterile water was added to make GHF11 strain liquid and GHF2 strain liquid respectively, and the volume ratio of sterile water to solid medium was 1.5:1;

[0067] (2) According to the mixed volume ratio of GHF11 strain liquid and GHF2 strain liquid as 2:1, mix the two strain liquids to obtain the mixed bacterial liquid, and then inoculate it into the liquid culture medium, the inoculation concentration of the mixed bacterial liquid is 2.5mL mixed bacteria liquid / 100mL liquid culture medium, fermented and cultured at 27°C for 5 days, and the shaking table rotated at 160r / min, to make a fermentation broth;

[0068] (3) Add t...

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Abstract

The invention relates to the technical field of water treatment, in particular to a method for preparing flocculating agents by using compound strains. The compound strains refer to Halomonas sp. GHF1and Psychrobacter sp. GHF2, the two strains are obtained through separation and purification from mud fluid spitted out by short-necked clam and are respectively subjected to amplification culture tobe prepared into two kinds of strain liquid, the strain liquid is mixed in a liquid medium for co-fermentation culture, flocculation carriers are added in fermented liquid in late fermentation, and exopolysaccharides is enabled to be settled completely by adding of ethyl alcohol. The strains are compounded and fermented and are cooperative with each other during fermentation, the strains are highin activity and yield of the exopolysaccharides, and the flocculation carriers are added in late fermentation; the strains are clumped on the flocculation carriers, settling of the exopolysaccharidesis promoted, use of the ethyl alcohol is reduced, and flocculation capacity of the flocculating agents is high and stable in performance.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to a method for preparing a flocculant by using a compound bacterial agent. Background technique [0002] my country's urbanization has developed rapidly in the past ten years, but due to the lag in investment in sewage treatment facilities, low sewage treatment efficiency, and the discharge of a large amount of industrial wastewater and domestic sewage, the serious water environment has deteriorated, and the content of heavy metals, pigments, and suspended particles in the water body exceeds the standard. The outbreak of algae such as blue-green algae, water resources have now become an important factor restricting the development of social urbanization and industrialization. Improving sewage treatment technology and strengthening urban sewage treatment capacity has become a top priority. Among them, introducing flocculent particles of appropriate particle size into the sew...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C02F1/56C12R1/01
Inventor 穆军崔霞张媛媛杨桥
Owner ZHEJIANG OCEAN UNIV
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