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Composition, medium and method for hyaluronic acid fermentation and application thereof

A technology of hyaluronic acid and medium, applied in the field of microbial fermentation, can solve the problems of high cost and insignificant effect of hyaluronic acid production, and achieve the effect of increasing HA production

Active Publication Date: 2021-05-04
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The problem of the prior art solved by the present invention is: the existing culture medium or fermentation method for producing hyaluronic acid by fermentation has no significant effect on increasing the production of hyaluronic acid, and the cost is relatively high

Method used

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  • Composition, medium and method for hyaluronic acid fermentation and application thereof
  • Composition, medium and method for hyaluronic acid fermentation and application thereof
  • Composition, medium and method for hyaluronic acid fermentation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0061] (1) Strain information

[0062] The Streptococcus equi subspecies zooepidemicus (also known as Streptococcus equisubsq.zooepidemicus) used in the present invention is commercially available. The address is in the campus of Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, China, postal code: 430072.

[0063] (2) Production method

[0064] (1) Slant culture: place the inoculated slant in a constant temperature incubator at 37° C. for 16 hours, and then perform shake flask inoculation.

[0065] (2) Seed culture: Inoculate the cultivated slant seeds into a 500mL Erlenmeyer flask containing 50mL seed medium for cultivation, the shaker speed is 200r / min, the temperature is 37°C, the cultivation time is 15h, and the pH is around 5.0-5.5 for inoculation.

[0066] (3) Fermentation culture: the seed culture medium is inserted into the fully automatic fermenter BioFlo115 type 3L reactor by the inoculum size of 10% (v / v), and the fermentation med...

Embodiment 2

[0072] The difference from Example 1 is that N-acetylglucosamine, aspartic acid and uridine diphosphate are added on the basis of the basic formula of the fermentation medium in Example 1, see Table 2 for the formula of Example 2. Wherein the concentration of N-acetylglucosamine in the fermentation medium is 1g / L, the concentration of aspartic acid is 3g / L, and the concentration of uridine diphosphate is 0.5g / L.

[0073] Wherein, the production method is as follows:

[0074] (1) Slant culture: place the inoculated slant in a constant temperature incubator at 37° C. for 16 hours, and then perform shake flask inoculation.

[0075] (2) Seed culture: Inoculate the cultivated slant seeds into a 500mL Erlenmeyer flask containing 50mL of seed medium for cultivation, the shaker speed is 300r / min, the temperature is 37°C, the cultivation time is 10h, and the pH is around 5.0-5.5 for inoculation.

[0076] (3) Fermentation culture: insert the seed culture medium into the fully automatic...

Embodiment 3

[0078] The difference from Example 1 is that N-acetylglucosamine, aspartic acid, uracil and uridine triphosphate are added on the basis of the basic formula of the fermentation medium in Example 1, see Table 2 Example 3 formula. The concentration of N-acetylglucosamine in the fermentation medium is 0.8g / L, the concentration of aspartic acid is 1g / L, the concentration of uracil is 1g / L, and the concentration of uridine triphosphate is 1g / L .

[0079] Wherein, the production method is as follows:

[0080] (1) Slant culture: place the inoculated slant in a constant temperature incubator at 37° C. for 16 hours, and then perform shake flask inoculation.

[0081] (2) Seed culture: inoculate the cultivated slant seeds into a 500mL Erlenmeyer flask containing 50mL seed medium for cultivation, the shaker speed is 100r / min, the temperature is 37°C, the cultivation time is 18h, and the pH is around 5.0-5.5 for inoculation.

[0082] (3) Fermentation culture: the seed medium is inserted...

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Abstract

The invention relates to the field of microbial fermentation, in particular to a hyaluronic acid fermentation composition, culture medium, method and application thereof. The composition includes N-acetylglucosamine and aspartic acid, and also contains one or more of uracil and its derivatives. The medium is supplemented with N-acetylglucosamine and aspartic acid, and also includes one or more of uracil and its derivatives. The present invention can effectively alleviate the problem of weak HA synthesis caused by the lack of competitive precursor substances in the process of HA synthesis by supplementing the precursor substances of HA synthesis in the culture medium. And the research results show that by adding the above precursor substances, the yield of HA can be increased by 10%-40%. The problem of low yield of hyaluronic acid in the fermentation process is solved. Moreover, the cost is controlled within a reasonable range, creating direct production benefits for production enterprises.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a hyaluronic acid fermentation composition, culture medium, method and application thereof. Background technique [0002] Hyaluronic acid, namely hyaluronic acid, referred to as HA, is an acidic mucopolysaccharide. The molecular structure is composed of two monosaccharides, N-acetylglucosamine and glucuronic acid, which are repeatedly and alternately connected. It is widely used in the food industry, cosmetics industry, and medicine industry. related fields, etc. [0003] At present, hyaluronic acid is mainly produced by microbial fermentation in industry, and the production strain is Streptococcus zooepidemicus. Streptococcus zooepidemicus is a nutritionally demanding lactis streptococcus, which is an auxotrophic bacterium for certain amino acids, including lysine, glutamic acid, arginine, cysteine, etc. Basic nutrients are also not adequately supplied. Since S. zooepide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26
CPCC12P19/26
Inventor 丁永志俞学锋李知洪李啸姚鹃伍业旭
Owner ANGELYEAST CO LTD