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A kind of composite stabilizer and application thereof for immunoglobulin m assay kit

A compound stabilizer and immunoglobulin technology, which is applied in biological testing, measuring devices, instruments, etc., can solve the problems of test strip stability, kit stability, and non-kit stability, etc., to ensure Accuracy, extended shelf life, low cost results

Active Publication Date: 2020-03-24
ZHONGSHAN CHUANGYI BIOCHEM ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN200810112740.9 and patent document CN201110394354.5 provide a method for making IgM antibody detection test strips, but do not add any stabilizers, and do not explain the stability of the test strips; patent document CN200810105741.0 provides a A chemiluminescence kit for detecting herpes simplex virus type I IgM antibody and its preparation method, also without adding any stabilizer, and did not explain the stability of the kit; patent CN200910265470.X provides a hepatitis C virus IgG, The IgM antibody joint detection kit and its preparation method also did not add any stabilizers, and did not explain the stability of the kit.

Method used

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  • A kind of composite stabilizer and application thereof for immunoglobulin m assay kit
  • A kind of composite stabilizer and application thereof for immunoglobulin m assay kit
  • A kind of composite stabilizer and application thereof for immunoglobulin m assay kit

Examples

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Effect test

Embodiment 1

[0031] A compound stabilizer for immunoglobulin M test kit, the content of the compound stabilizer components is: 10g / L bovine serum albumin, 10g / L propylene glycol, 1g / L Triton X-100, 15g / L Lactose, 2g / L liquid biological preservative Proclin300.

[0032] The preparation method of above-mentioned composite stabilizer, comprises the following steps:

[0033] (1) Add the weighed bovine serum albumin and propylene glycol successively in the dissolving bucket, add an appropriate amount of water and stir the lactose to dissolve it completely;

[0034] (2) Then add Triton X-100 to fully emulsify the above raw materials;

[0035] (3) After the emulsification is complete, add Proclin300 and sufficient water, stir to dissolve completely, seal the reaction barrel with a lid, and filter to obtain the product.

Embodiment 2

[0037] A compound stabilizer for immunoglobulin M test kit, the content of the compound stabilizer component is: 3g / L bovine serum albumin, 4g / L propylene glycol, 1g / L Triton X-100, 1.5g / L L lactose, 0.15g / L liquid biological preservative Proclin300.

[0038] The preparation method of above-mentioned composite stabilizer, comprises the following steps:

[0039] (1) Add the weighed bovine serum albumin and propylene glycol successively in the dissolving bucket, add an appropriate amount of water and stir the lactose to dissolve it completely;

[0040] (2) Then add Triton X-100 to fully emulsify the above raw materials;

[0041] (3) After the emulsification is complete, add Proclin300 and sufficient water, stir to dissolve completely, seal the reaction barrel with a lid, and filter to obtain the product.

Embodiment 3

[0043] A compound stabilizer for immunoglobulin M test kit, the content of the compound stabilizer components is: 1g / L bovine serum albumin, 5g / L propylene glycol, 1g / L Triton X-100, 2g / L Lactose, 0.1g / L liquid biological preservative Proclin300.

[0044] The preparation method of above-mentioned composite stabilizer, comprises the following steps:

[0045] (1) Add the weighed bovine serum albumin and propylene glycol successively in the dissolving bucket, add an appropriate amount of water and stir the lactose to dissolve it completely;

[0046] (2) Then add Triton X-100 to fully emulsify the above raw materials;

[0047](3) After the emulsification is complete, add Proclin300 and sufficient water, stir to dissolve completely, seal the reaction barrel with a lid, and filter to obtain the product.

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PUM

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Abstract

The invention discloses a complex stabilizer for immunoglobulin M measuring reagent kits. The complex stabilizer comprises, by weight, 1-5% of blocking agents, 1-2.5% of lactose, 1-2% of surfactants,1-1.5% of emulsifying agents, 0.1-0.2% of liquid biological preservatives and the balance purified water. The complex stabilizer can be applied to the immunoglobulin M measuring reagent kits, and theusage amount of the complex stabilizer accounts for 3-5% of the volume fraction of reagents in the reagent kits. The complex stabilizer has the advantages that the complex stabilizer is easy to prepare and convenient to operate, and the accuracy of detection results of the reagent kits can be guaranteed; the complex stabilizer can be stably applied to the immunoglobulin M measuring reagent kits, and the storage time of the immunoglobulin M measuring reagent kits can be effectively prolonged.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis, and in particular relates to a composite stabilizer used in an immunoglobulin M (IgM) assay kit, a preparation method and an application thereof. Background technique [0002] Immunoglobulin refers to animal protein with antibody activity, which mainly exists in plasma, and is also found in other body fluids, tissues and some secretions. It can be divided into five categories, namely, immunoglobulin G, immunoglobulin A, immunoglobulin M, immunoglobulin D and immunoglobulin A. Among them, immunoglobulin M (immunoglobulin, IgM) is a type of globulin with antibody activity or antibody-like structure synthesized and secreted by B lymphocytes after antigen induction, differentiation into plasma cells, and is the main substance mediating humoral immune response . IgM is the largest molecular weight among the five classes of immunoglobulins, with a molecular weight of 9×10 5 Dalton, so it is also ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6854
Inventor 何林辉崔海林曾晓君
Owner ZHONGSHAN CHUANGYI BIOCHEM ENG