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A method for establishing an intestinal sterile experimental model of red palm weevil larvae

A red palm weevil and experimental model technology, applied in the field of biology, can solve problems such as hindering the research on the molecular physiology mechanism of insects and their intestinal flora, and cannot completely remove the intestinal flora of insects, etc., to promote the research of molecular physiology mechanism Effect

Inactive Publication Date: 2020-06-09
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited efficacy of antibiotics and the influence of environmental factors in the insect gut, the method of antibiotic feeding cannot completely remove the intestinal flora of insects, which to some extent hinders the synergistic interaction between insects and their intestinal flora The study of the molecular physiological mechanism of

Method used

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  • A method for establishing an intestinal sterile experimental model of red palm weevil larvae
  • A method for establishing an intestinal sterile experimental model of red palm weevil larvae

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Experimental program
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Effect test

Embodiment 1

[0034] A method for creating an intestinal sterile red palm weevil larvae experimental model, comprising the following steps:

[0035] 1) Use the red palm weevil population in the laboratory to collect fresh eggs produced by red palm weevil females within 24 hours;

[0036] 2) Soak the collected fresh eggs in 11% sodium hypochlorite solution for 3 minutes to remove the bacteria on the surface of the eggs;

[0037] 3) Rinse the eggs soaked in sodium hypochlorite solution three times with 75% ethanol to further remove bacteria that may remain on the surface of the eggs;

[0038] 4) Rinse the eggs rinsed with ethanol with sterile ultrapure water, wash off the ethanol solution that may remain on the surface of the eggs, and then transfer the rinsed eggs to a sterile artificial feed for the red palm weevil In a clean and sterilized glass petri dish (diameter 9cm);

[0039] 5) Place an incubator in a biological safety cabinet, place the above-mentioned eggs that have been transfer...

Embodiment 2

[0041] A method for creating an intestinal sterile red palm weevil larvae experimental model, comprising the following steps:

[0042] 1) Use the red palm weevil population in the laboratory to collect fresh eggs produced by red palm weevil females within 24 hours;

[0043] 2) Soak the collected fresh eggs in 11% sodium hypochlorite solution for 3 minutes to remove the bacteria on the surface of the eggs;

[0044] 3) Rinse the eggs soaked in sodium hypochlorite solution three times with 75% ethanol to further remove bacteria that may remain on the surface of the eggs;

[0045] 4) Rinse the eggs rinsed with ethanol with sterile ultrapure water, wash off the ethanol solution that may remain on the surface of the eggs, and then transfer the rinsed eggs to a sterile artificial feed for the red palm weevil In a clean and sterilized glass petri dish (diameter 9cm);

[0046] 5) Place an incubator in a biological safety cabinet, place the above-mentioned eggs that have been transfer...

Embodiment 3

[0048] A method for creating an intestinal sterile red palm weevil larvae experimental model, comprising the following steps:

[0049] 1) Use the red palm weevil population in the laboratory to collect fresh eggs produced by red palm weevil females within 24 hours;

[0050] 2) Soak the collected fresh eggs in a 10% sodium hypochlorite solution for 4 minutes to remove bacteria on the surface of the eggs;

[0051] 3) Rinse the eggs soaked in sodium hypochlorite solution twice with 75% ethanol to further remove bacteria that may remain on the surface of the eggs;

[0052] 4) Rinse the eggs rinsed with ethanol with sterile ultrapure water, wash off the ethanol solution that may remain on the surface of the eggs, and then transfer the rinsed eggs to a sterile artificial feed for the red palm weevil In a clean and sterilized glass petri dish (diameter 9cm);

[0053] 5) Place an incubator in a biological safety cabinet, place the above-mentioned eggs that have been transferred to the...

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Abstract

The invention discloses an establishing method for an intestinal-aseptic rhynchophorus-ferrugineus larva experimental model. The establishing method includes the steps that important invasive pest rhynchophorus ferrugineus on palm plants in our country serves as an object, collected rhynchophorus-ferrugineus egg granules are soaked with a sodium hypochlorite solution, and then are subjected to a series of technology treatment such as elution 2 times to 3 times with ethyl alcohol and sterile water in sequence to remove bacteria on the surfaces of rhynchophorus-ferrugineus eggs, the obtained surface-aseptic eggs after treatment are transferred to aseptic artificial feed, and then are put into a biological safety cabinet to be cultivated and hatched into larvae, and therefore the intestinal-aseptic rhynchophorus-ferrugineus experimental model is obtained. On this basis, obtained intestinal bacteria after separation are added into the aseptic artificial feed, specific intestinal bacteria are newly introduced into the intestinal-aseptic rhynchophorus-ferrugineus experimental model, and the experimental model with the specific intestinal bacteria is obtained. In this way, as the intestinal-aseptic rhynchophorus-ferrugineus larva experimental model is established, molecular physiology mechanism research of synergistic interactions between the rhynchophorus ferrugineus and the intestinal flora of the rhynchophorus ferrugineus is fundamentally and greatly promoted.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for creating an intestinal sterile experimental model of the red palm weevil larvae. Background technique [0002] The red palm weevil is a major invasive pest on palm plants in my country. There is a rich bacterial community in the intestinal tract of this pest, and evidence has shown that these intestinal bacteria play a role in the physiological processes such as nutrient metabolism of the red palm weevil. plays an important role in. In order to explore the molecular physiological mechanism of the synergistic interaction between the red palm weevil and its intestinal flora, the most commonly used method is to eliminate the intestinal flora by feeding antibiotics. However, due to the limited efficacy of antibiotics and the influence of environmental factors in the insect gut, the method of antibiotic feeding cannot completely remove the intestinal flora of insects,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 石章红侯有明阿布拉·穆罕默德黄斌
Owner FUJIAN AGRI & FORESTRY UNIV