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Separation method of Amomum villosum pollen germ cells

A germ cell and separation method technology is applied in the field of separation of germ cells from Amomum vulgaris pollen, which can solve the problems of shortage of Chinese medicinal materials Amomum vulgaris, influence on product development and resource application of Amomum vulgaris, low economic benefit, etc. Effect

Active Publication Date: 2018-02-09
ZHANG ZHOU HALTH VOCATIONAL COLLEGE
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Problems solved by technology

[0003] Due to the particularity of the flower organ structure of Yangchunsha, that is, the stigma is higher than the anther, the natural pollination rate is very low, only 3% to 8%, the seed setting rate and unit yield are greatly affected, the economic benefit is low, and the planting area decreases year by year. This has led to the shortage of amomum, a traditional Chinese medicinal material, which has affected the development of amomum products and the application of resources, such as the production of Chinese patent medicines such as "Xiangsha Liujun pills" and "Xiangsha Yangwei pills", as well as some amomum health products, food, cosmetics, and seasonings Development of products such as medicines and medicinal liquors

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  • Separation method of Amomum villosum pollen germ cells
  • Separation method of Amomum villosum pollen germ cells

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Embodiment Construction

[0027] Materials and Methods

[0028] The experimental material comes from the planting place of Amomum villosum Lour. in Zudi Village, Chenxiang Town, Changtai County, Zhangzhou City, Fujian Province. It is identified as Amomum villosum Lour., which is a perennial herb of the genus Cardamom in the family Zingiberaceae. Yangchunsha usually blooms from late May to early June. Spikes grow from underground creeping rhizomes and bloom on the surface of the ground. The inflorescence is elliptical, consisting of 5-8 flowers; the corolla is white, the lips are obovate, the midvein is yellow and slightly raised; the pistil is 1, the style is sandwiched between two anthers, and the stigma is higher than the anthers ( Picture 1-1 ).

[0029] Put the freshly cracked Anthera anthers in different concentration gradients of mannitol, plus 5% acetone mixture, the pollen absorbs water and swells, ruptures and releases the pollen cytoplasm, which contains germ cells, to achieve the purpose o...

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Abstract

The invention discloses a separation method of Amomum villosum pollen germ cells. The separation method comprises following steps: Amomum villosum flower anthers right after cracking are immersed in amixed solution of mannitol and acetone, expansion of pollen after water absorption is realized, pollen cytoplasm containing germ cells is released after breaking of pollen, and the germ cells are separated using a microscope. The separation method is capable of realizing separation of Amomum villosum pollen germ cells, and maintaining the activity of the germ cells.

Description

technical field [0001] The invention relates to a method for isolating germ cells of yangchunsha pollen. Background technique [0002] Amomum is a famous and precious traditional Chinese medicine that is short of resources in our country. It is used for both medicine and food, and its ripe fruit is used as medicine or edible. Spicy in taste and warm in nature, it has the functions of dispelling dampness and appetizing, warming spleen and stopping diarrhea, regulating qi and preventing miscarriage. The sources of Chinese herbal medicine Amomum villosum contained in "Chinese Pharmacopoeia" (2015 edition) are Amomum villosum Lour., Amomum villosum var. xanthioides T.L.Wu et Senjen or Amomum longiligulare T.L.Wu of dry ripe fruit. Amomum amomum is the source plant with the best quality, and it is also the mainstream species of Amomum japonicus in Fujian Province. The sixth volume of "Fujian Flora" records: Amomum, also known as Yangchun Amomum, Changtai Amomum, the fruit is m...

Claims

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Application Information

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IPC IPC(8): C12N5/04
CPCC12N5/04
Inventor 林美珍
Owner ZHANG ZHOU HALTH VOCATIONAL COLLEGE